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Dopamine receptor Dop1R2 stabilizes appetitive olfactory memory through the Raf/MAPK pathway

Sun Huan 東北大学

2020.09.25

概要

In Drosophila, dopamine signaling to the mushroom body intrinsic neurons, Kenyon cells (KCs), is critical to stabilize olfactory memory. Nevertheless, little is known about the downstream intracellular molecular signaling underlying memory stabilization. Here I address this question in the context of sugar-rewarded olfactory long-term memory (LTM). I show that associative conditioning increases the phosphorylation of mitogen-activated protein kinase (MAPK) in KCs, which process relies on the presence of the dopamine receptor Dop1R2. Consistently, the attenuation of Dop1R2, Raf (a kinase) or MAPK expression in KCs selectively impairs LTM but not short-term memory. Moreover, I show that the LTM deficit caused by the knockdown of Dop1R2 can be rescued by expressing active Raf in KCs. Thus, I conclude that the Dop1R2/Raf/MAPK pathway is a pivotal downstream effector of dopamine signaling for stabilizing appetitive olfactory memory.

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