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大学・研究所にある論文を検索できる 「Purification of human iPSC-derived cells at large scale using microRNA switch and magnetic-activated cell sorting」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。
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Purification of human iPSC-derived cells at large scale using microRNA switch and magnetic-activated cell sorting

Tsujisaka, Yuta Hatani, Takeshi Okubo, Chikako Ito, Ryo Kimura, Azuma Narita, Megumi Chonabayashi, Kazuhisa Funakoshi, Shunsuke Lucena-Cacace, Antonio Toyoda, Taro Osafune, Kenji Kimura, Takeshi Saito, Hirohide Yoshida, Yoshinori 京都大学 DOI:10.1016/j.stemcr.2022.05.003

2022.07.12

概要

For regenerative cell therapies using pluripotent stem cell (PSC)-derived cells, large quantities of purified cells are required. Magnetic-activated cell sorting (MACS) is a powerful approach to collect target antigen-positive cells; however, it remains a challenge to purify various cell types efficiently at large scale without using antibodies specific to the desired cell type. Here we develop a technology that combines microRNA (miRNA)-responsive mRNA switch (miR-switch) with MACS (miR-switch-MACS) to purify large amounts of PSC-derived cells rapidly and effectively. We designed miR-switches that detect specific miRNAs expressed in target cells and controlled the translation of a CD4-coding transgene as a selection marker for MACS. For the large-scale purification of induced PSC-derived cardiomyocytes (iPSC-CMs), we transferred miR-208a-CD4 switch-MACS and obtained purified iPSC-CMs efficiently. Moreover, miR-375-CD4 switch-MACS highly purified pancreatic insulin-producing cells and their progenitors expressing Chromogranin A. Overall, the miR-switch-MACS method can efficiently purify target PSC-derived cells for cell replacement therapy.

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