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SUPPLEMENTARY MATERIAL ONLINE
Supplementary materials and methods
Figure S1. Gene recombination in extrahepatic biliary epithelial cells confirmed using X-gal staining in biliary tract of Pdx1-Cre; Rosa26LSL-LacZ mice
Figure S2. ELF3 is not essential for development and maintenance of extrahepatic biliary tract in mice
Figure S3. Elf3 deletion does not cause tumour formation in gallbladder in context of Trp53 mutation in mice
Figure S4. Genotyping and RT-qPCR analysis for verification of origin of organoids
Figure S5. EGFR signalling regulates mTORC1 activity through PI3K/AKT and AMPK
Figure S6. Dedifferentiation and epithelial–mesenchymal transition (EMT) are induced in KPCE allograft tumour cells in vitro
Figure S7. EGFR/mTORC1 inhibition suppresses cell proliferation and EMT in KPCE organoids
Figure S8. Gene sets related to EGFR, mTOR, and EMT are significantly enriched in human GBC tissues with ELF3 mutations
Table S1. Clinical information of 85 patients with GBC
Table S2. Antibodies for IHC, western blotting, and ChIP assay used in this study
Table S3. Primer sets for RT-qPCR, ChIP, and genotyping analyses used in this study
© 2023 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd
on behalf of The Pathological Society of Great Britain and Ireland. www.pathsoc.org
J Pathol 2023; 261: 28–42
www.thejournalofpathology.com
10969896, 2023, 1, Downloaded from https://pathsocjournals.onlinelibrary.wiley.com/doi/10.1002/path.6144 by Cochrane Japan, Wiley Online Library on [19/09/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License
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