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英文抄録

Koji culture of actinomycetes for long-term storage

Shohei Ebe1,

Masatoshi Sakazaki1,2,

Tatsuya Ohike1,

Masahiro Okanami1,2

and

Takashi Ano1,2

Actinomycetes, Gram-positive bacteria, produce a variety of secondary metabolites, including antibiotics. Many

beneficial compounds in our life have been discovered from actinomycetes so far and many researchers attempt to

isolate new natural compounds. However, it has been known that an antibiotic-producing ability of the actinomycetes

is reduced by repeated passages onto fresh growth medium. Thus, it is necessary to establish an alternative method

for a long-term storage of the actinomycetes with high antibiotic-producing ability. We focused on a koji-making

process which is performed by solid state fermentation using steamed rice with Aspergillus oryzae (koji culture) and

tried to make the cultured rice medium with actinomycetes to maintain the antibiotic-producing ability during the

storage period. In this study, we performed the rice culture of actinomycetes using low-grade rice and evaluated the

usefulness of the culture method for maintaining the antibiotic-producing ability. The rice culture medium was

prepared by autoclave of a beaker containing water-soaked rice. Each of twelve actinomycete strains with an

antibiotic-producing ability was inoculated and cultured in the rice culture medium. As the result of cultivation, it

was observed that all actinomycete strains could grow on the surface of rice grains in the beakers. After three months

of cultivation, a piece of the cultured rice media of actinomycetes was retrieved from the beaker and was used for an

antifungal activity test on an agar plate. All actinomycetes formed colonies on agar plates and showed antifungal

activities against a plant pathogenic fungus. These results suggest that the method of koji culture of actinomycetes is

effective in maintaining the antibiotic-producing ability.

Key words : Actinomycete, solid state fermentation, antibiotic-producing ability, koji culture

Received 30 July 2020, Accepted 22 October 2020.

1. Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan

2. Major in Biotechnological Science, Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493

Japan.

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