Peripartum changes in serum activities of three major alkaline phosphatase isoenzymes in Holstein dairy cows

概要

A commercial agarose gel electrophoresis (AGE)
kit can discriminate among three major alkaline phosphatase (ALP) isoenzymes in bovine sera, namely,
a hepatic ALP isoenzyme derived from hepatic tissue
(ALP2), a bone ALP isoenzyme derived from osteoblasts (ALP3), and an intestinal ALP isoenzyme derived
from intestinal tissue (ALP5) (Chiba et al. 2020).
We recently reported that the AGE method using both
protease- and protease and neuraminidase-treated sera
could be used to measure serum activities of these isoenzymes in lactating Holstein cows (Chiba et al. 2020).
Dairy cows often suffer from metabolic diseases such
as ketosis and hypocalcemia after parturition; these
may result from hepatic dysfunction due to mobilized
body fat and inhibited bone metabolism (Goff and Horst
1997). Therefore, this study investigated changes
in circulating ALP isoenzyme activities in cows around
parturition. ...

この論文で使われている画像