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158

山形大学紀要（農学）第 18 巻 第 4 号

イネにおける米糠タンパク質のプロテオミクス

阿部利徳・滝澤虎三郎・長澤和永・笹沼恒男

山形大学農学部食料生命環境学科

（令和 2 年 9 月 4 日受付・令和 2 年 11 月 10 日受理）

米糠は、その栄養価の高さから、広く利用されること

ンド型の TN-1 の米糠から抽出した、それぞれ混合タン

が期待されるが、その構成タンパク質に関してはほとん

パク質の液相内トリプシン消化により、ペプチド混合物

ど知られていない。本研究では、プロテオミクスの手法

を得て LC-MS/MS 分析を行った。両品種に共通する 83

で含有する米糠タンパク質を同定した。コシヒカリ米糠

種のタンパク質が検出され、それらを 10 のカテゴリーに

タンパク質の二次元電気泳動法により、41 のタンパク質

分類した：タンパク質の種類の多い順に、貯蔵タンパク

スポットが分離でき、そのうち 23 のスポットの N 末端ア

質、炭水化物代謝、タンパク質合成と分解、胚特異的、

ミノ酸配列が決定できた。そのうちの 21 スポットは

ストレス誘導、調節、抗酸化、酸化還元、脂質合成など

Blast 検索によりタンパク質が同定できたが、２スポット

である。これらの同定したタンパク質のうち、抗酸化作

は機能未知であった。他の 18 スポットの N 末端アミノ酸

用のある、パーオキシレドキシンや、酸化還元に関係し

配列データは得られなかった。そのうちの 4 スポットに

たグリオキサラーゼなどは登熟期および収穫後の乾燥な

ついて、トリプシンによるゲル内消化後の LC-MS/MS 分

どによるストレス下で、種子の胚や米粉層細胞などで、

析により同定した。次に、イネ日本型のコシヒカリとイ

細胞を防御する役割を果たしていることが推察される。

キーワード：イネ，プロテオミクス，米糠，胚，アリューロン，アミノ酸配列，LC-MS/MS

350

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