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歯周炎検出のための唾液中歯肉縁下プラーク特異的細菌の臨床的有用性

馬, 佳楽 MA, JIALE マ, カラク 九州大学

2023.03.20

概要

九州大学学術情報リポジトリ
Kyushu University Institutional Repository

Clinical utility of subgingival plaque-specific
bacteria in salivary microbiota for detecting
periodontitis
馬, 佳楽

https://hdl.handle.net/2324/6787532
出版情報：九州大学, 2022, 博士（歯学）, 課程博士
バージョン：
権利関係：© 2021 Ma et al. This is an open access article distributed under the terms of the
Creative Commons Attribution License.

（様式３）

氏

名

：馬

佳楽

論 文 名

：Clinical utility of subgingival plaque-specific bacteria in salivary microbiota
for detecting periodontitis
（歯周炎検出のための唾液中歯肉縁下プラーク特異的細菌の臨床的
有用性）

区

：甲

分

論

文

内

容

の

要

旨

Saliva contains diverse bacteria shed from various oral sites, including subgingival plaque. It is
reasonable to focus on the total occupancy of subgingival plaque-specific bacteria (SUBP
bacteria), which live in subgingival environments, in the saliva for detecting periodontitis using
salivary testing. This study aimed to validate the clinical utility of SUBP bacteria in the
salivary microbiota for the detection of periodontitis. We examined stimulated saliva samples
collected from 125 subjects who visited three dental clinics. The relative abundances of
previously identified 11 SUBP bacteria were determined using 16S ribosomal RNA gene
sequencing and a reference-based approach. The prediction performance was evaluated using a
receiver operating characteristic (ROC) curve. The SUBP bacteria accounted for 0-15.4% of the
salivary microbiota, and the percentage distinguished periodontitis patients with at least 15
sites with probing depth ≥4 mm with a sensitivity of 0.90 (95% confidence interval [CI],
0.81-0.98) and specificity of 0.70 (95% CI, 0.60-0.80) (area under the ROC curve [AUC], 0.87).
Among 2,047 combinations of 11 SUBP bacteria, combinations including Streptococcus
constellatus, Porphyromonas gingivalis, and Fusobacterium nucleatum subsp. vincentii
demonstrated significantly higher AUC values in their detection. These results suggest that
examining SUBP bacteria in saliva may be useful for detecting periodontitis patients in mass
screening.

(Fig 2) AUC values using the total relative
(Fig 1) ROC curves for prediction of periodontal status

abundance of 2,047 combinations of the SUBP
bacteria

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