論文の公開元へ

書き出し

Refer/BibIX

RIS

BibTeX

TSV

Isolation of tumor endothelial cells from murine cancer

田口和浩広島大学

2020.02.27

概要

During the development of tumor microenvironment, various types
of cells in the tumor stroma aﬀect tumor growth. Tumor endothelial
cells (TECs), which constitute the lining of the tumor blood vessels, not
only contribute to supply blood to tumors, but have various characteristics as tumor constituent cells. Tumors construct an abnormal
vasculature during tumor angiogenesis. It has been indicated that the
morphology and gene expression of TECs diﬀer from those of normal
endothelial cells (St Croix et al., 2000; Jain, 2003; Lu et al., 2007; Nagy
et al., 2009; Hida et al., 2011; Hida et al., 2013). TECs might also have
aberrant growth, migratory, and diﬀerentiation abilities (Ghosh et al.,
2008; Dunleavey et al., 2014), and they might acquire those characteristics as tumor microenvironment is established (Dudley, 2012;
Ohga et al., 2012). Furthermore, there are some reports indicating that
TECs might be able to acquire resistance to drugs (Bussolati et al., 2003;
Grange et al., 2006; Xiong et al., 2009; Akiyama et al., 2012). ...

論文の公開元へ

この論文で使われている画像

参考文献

Akiyama, K., Ohga, N., Hida, Y., Kawamoto, T., Sadamoto, Y., Ishikawa, S., Maishi, N.,

Akino, T., Kondoh, M., Matsuda, A., Inoue, N., Shindoh, M., Hida, K., 2012. Tumor

endothelial cells acquire drug resistance by MDR1 up-regulation via VEGF signaling

in tumor microenvironment. Am. J. Pathol. 180, 1283–1293.

Amin, D.N., Hida, K., Bielenberg, D.R., Klagsbrun, M., 2006. Tumor endothelial cells

express epidermal growth factor receptor (EGFR) but not ErbB3 and are responsive to

EGF and to EGFR kinase inhibitors. Cancer Res. 66, 2173–2180.

Burrows, F.J., Derbyshire, E.J., Tazzari, P.L., Amlot, P., Gazdar, A.F., King, S.W., Letarte,

M., Vitetta, E.S., Thorpe, P.E., 1995. Up-regulation of endoglin on vascular endothelial cells in human solid tumors: implications for diagnosis and therapy. Clin.

Cancer Res. 1, 1623–1634.

Bussolati, B., Deambrosis, I., Russo, S., Deregibus, M.C., Camussi, G., 2003. Altered angiogenesis and survival in human tumor-derived endothelial cells. FASEB J. 17,

1159–1161.

Carson-Walter, E.B., Watkins, D.N., Nanda, A., Vogelstein, B., Kinzler, K.W., St Croix, B.,

2001. Cell surface tumor endothelial markers are conserved in mice and humans.

Cancer Res. 61, 6649–6655.

Dudley, A.C., 2012. Tumor endothelial cells. Cold Spring Harbor Perspect. Med. 2,

a006536.

Dudley, A.C., Khan, Z.A., Shih, S.C., Kang, S.Y., Zwaans, B.M., Bischoﬀ, J., Klagsbrun, M.,

2008. Calciﬁcation of multipotent prostate tumor endothelium. Cancer Cell 14,

201–211.

Dunleavey, J.M., Xiao, L., Thompson, J., Kim, M.M., Shields, J.M., Shelton, S.E., Irvin,

D.M., Brings, V.E., Ollila, D.W., Brekken, R.A., Dayton, P.A., Melero-Martin, J.M.,

Dudley, A.C., 2014. Vascular channels formed by subpopulations of PECAM1+

melanoma cells. Nat. Commun. 5, 5200.

Ghosh, K., Thodeti, C.K., Dudley, A.C., Mammoto, A., Klagsbrun, M., Ingber, D.E., 2008.

Tumor-derived endothelial cells exhibit aberrant Rho-mediated mechanosensing and

abnormal angiogenesis in vitro. Proc. Nat. Acad. Sci. U. S. A. 105, 11305–11310.

Grange, C., Bussolati, B., Bruno, S., Fonsato, V., Sapino, A., Camussi, G., 2006. Isolation

and characterization of human breast tumor-derived endothelial cells. Oncol. Rep.

15, 381–386.

Hellebrekers, D.M., Melotte, V., Vire, E., Langenkamp, E., Molema, G., Fuks, F., Herman,

J.G., Van Criekinge, W., Griﬃoen, A.W., van Engeland, M., 2007. Identiﬁcation of

epigenetically silenced genes in tumor endothelial cells. Cancer Res. 67, 4138–4148.

112

Journal of Immunological Methods 464 (2019) 105–113

K. Taguchi et al.

a selective immune barrier promoting tolerance in tumors. Nat. Med. 20, 607–615.

Nagy, J.A., Chang, S.H., Dvorak, A.M., Dvorak, H.F., 2009. Why are tumour blood vessels

abnormal and why is it important to know? Br. J. Cancer 100, 865–869.

Ohga, N., Ishikawa, S., Maishi, N., Akiyama, K., Hida, Y., Kawamoto, T., Sadamoto, Y.,

Osawa, T., Yamamoto, K., Kondoh, M., Ohmura, H., Shinohara, N., Nonomura, K.,

Shindoh, M., Hida, K., 2012. Heterogeneity of tumor endothelial cells: comparison

between tumor endothelial cells isolated from high- and low-metastatic tumors. Am.

J. Pathol. 180, 1294–1307.

Onoe, T., Ohdan, H., Tokita, D., Hara, H., Tanaka, Y., Ishiyama, K., Asahara, T., 2005a.

Liver sinusoidal endothelial cells have a capacity for inducing nonresponsiveness of T

cells across major histocompatibility complex barriers. Transpl. Int. 18, 206–214.

Onoe, T., Ohdan, H., Tokita, D., Shishida, M., Tanaka, Y., Hara, H., Zhou, W., Ishiyama,

K., Mitsuta, H., Ide, K., Asahara, T., 2005b. Liver sinusoidal endothelial cells tolerize

T cells across MHC barriers in mice. J. Immunol. 175, 139–146.

St Croix, B., Rago, C., Velculescu, V., Traverso, G., Romans, K.E., Montgomery, E., Lal, A.,

Riggins, G.J., Lengauer, C., Vogelstein, B., Kinzler, K.W., 2000. Genes expressed in

human tumor endothelium. Science 289, 1197–1202.

Thijssen, V.L., Hulsmans, S., Griﬃoen, A.W., 2008. The galectin proﬁle of the endothelium: altered expression and localization in activated and tumor endothelial

cells. Am. J. Pathol. 172, 545–553.

Xiao, L., McCann, J.V., Dudley, A.C., 2015. Isolation and culture expansion of tumorspeciﬁc endothelial cells. J. Vis. Exp. e53072.

Xiong, Y.Q., Sun, H.C., Zhang, W., Zhu, X.D., Zhuang, P.Y., Zhang, J.B., Wang, L., Wu,

W.Z., Qin, L.X., Tang, Z.Y., 2009. Human hepatocellular carcinoma tumor-derived

endothelial cells manifest increased angiogenesis capability and drug resistance

compared with normal endothelial cells. Clin. Cancer Res. 15, 4838–4846.

Hendry, S.A., Farnsworth, R.H., Solomon, B., Achen, M.G., Stacker, S.A., Fox, S.B., 2016.

The role of the tumor vasculature in the host immune response: Implications for

therapeutic strategies targeting the tumor microenvironment. Front. Immunol. 7,

621.

Hida, K., Kawamoto, T., Ohga, N., Akiyama, K., Hida, Y., Shindoh, M., 2011. Altered

angiogenesis in the tumor microenvironment. Pathol. Int. 61, 630–637.

Hida, K., Ohga, N., Akiyama, K., Maishi, N., Hida, Y., 2013. Heterogeneity of tumor endothelial cells. Cancer Sci. 104, 1391–1395.

Jain, R.K., 2003. Molecular regulation of vessel maturation. Nat. Med. 9, 685–693.

Kajimoto, K., Hossen, M.N., Hida, K., Ohga, N., Akita, H., Hyodo, M., Hida, Y., Harashima,

H., 2010. Isolation and culture of microvascular endothelial cells from murine inguinal and epididymal adipose tissues. J. Immunol. Methods 357, 43–50.

Lu, C., Bonome, T., Li, Y., Kamat, A.A., Han, L.Y., Schmandt, R., Coleman, R.L.,

Gershenson, D.M., Jaﬀe, R.B., Birrer, M.J., Sood, A.K., 2007. Gene alterations identiﬁed by expression proﬁling in tumor-associated endothelial cells from invasive

ovarian carcinoma. Cancer Res. 67, 1757–1768.

Marelli-Berg, F.M., Peek, E., Lidington, E.A., Stauss, H.J., Lechler, R.I., 2000. Isolation of

endothelial cells from murine tissue. J. Immunol. Methods 244, 205–215.

Matsuda, K., Ohga, N., Hida, Y., Muraki, C., Tsuchiya, K., Kurosu, T., Akino, T., Shih, S.C.,

Totsuka, Y., Klagsbrun, M., Shindoh, M., Hida, K., 2010. Isolated tumor endothelial

cells maintain speciﬁc character during long-term culture. Biochem. Biophys. Res.

Commun. 394, 947–954.

Mauge, L., Terme, M., Tartour, E., Helley, D., 2014. Control of the adaptive immune

response by tumor vasculature. Front. Oncol. 4, 61.

Motz, G.T., Santoro, S.P., Wang, L.P., Garrabrant, T., Lastra, R.R., Hagemann, I.S., Lal, P.,

Feldman, M.D., Benencia, F., Coukos, G., 2014. Tumor endothelium FasL establishes

113

...

参考文献をもっと見る

分野

大学

学位論文種類・取得年

言語

Isolation of tumor endothelial cells from murine cancer

概要

この論文で使われている画像

関連論文