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Requirement for the collagen receptor Endo180 in collagen gel contraction mediated by corneal fibroblasts

西田 功一 近畿大学

2022.02.28

概要

The interaction of keratocytes with extracellular matrix components plays an important role in the maintenance of corneal transparency and shape as well as in the healing of corneal wounds. In particular, the interaction of these cells with collagen and cell-mediated collagen contraction contribute to wound closure. Endo180 is a receptor for collagen that mediates its cellular internalization. We have now examined the role of Endo180 in collagen contraction mediated by corneal fibroblasts （activated keratocytes）. Antibodies to Endo180 inhibited the contractile activity of mouse corneal fibroblasts embedded in a three-dimensional collagen gel and cultured in the presence of serum, with this effect being both concentration and time dependent and essentially complete at an antibody concentration of 0.2 µg/ml. Whereas corneal fibroblasts cultured in a collagen gel manifested a flattened morphology with prominent stress fibers under control conditions, they showed a spindlelike shape with few stress fibers in the presence of antibodies to Endo180. Antibodies to Endo180 had no effect on the expression of α–smooth muscle actin or the extent of collagen degradation in collagen gel cultures of corneal fibroblasts. Immunohistofluorescence analysis did not detect the expression of Endo180 in the unwounded mouse cornea. However, Endo180 expression was detected in keratocytes migrating into the wound area at 3 days after a corneal incisional injury. Together, our results suggest that Endo180 is required for the contraction of collagen matrix mediated by corneal fibroblasts and that its expression in these cells may contribute to the healing of corneal stromal wounds.

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参考文献

［ １ ］Nishida, T., Saika, S., Morishige, N., 2017. Cornea and sclera: anatomy and physiology, in: Mannis, M.J., Holland, E.J （Eds.）, Cornea, 4th ed. Elsevier, New York, pp. 1–22.

［ ２ ］Chen, S., Mienaltowski, M.J., Birk, D.E., 2015. Regulation of corneal stroma extracellular matrix assembly. Exp. Eye Res. 133, 69–80.

［ ３ ］Hassell, J.R., Birk, D.E., 2010. The molecular basis of corneal transparency. Exp. Eye Res. 91, 326–335.

［ ４ ］Bell, E., Ivarsson, B., Merrill, C., 1979. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc. Natl. Acad. Sci. USA 76, 1274–1278.

［ ５ ］Grinnell, F., 2003. Fibroblast biology in three-dimensional collagen matrices. Trends Cell Biol. 13, 264–269.

［ ６ ］Nishida, T., Ueda, A., Fukuda, M., Mishima, H., Yasumoto, K., Otori, T., 1988. Interactions of extracellular collagen and corneal fibroblasts: morphologic and biochemical changes of rabbit corneal cells cultured in a collagen matrix. In Vitro Cell. Dev. Biol. 24, 1009–1014.

［ ７ ］Assouline, M., Chew, S.J., Thompson, H.W., Beuerman, R., 1992. Effect of growth factors on collagen lattice contraction by human keratocytes. Invest. Ophthalmol. Vis. Sci. 33, 1742–1755.

［ ８ ］Kurosaka, H., Kurosaka, D., Kato, K., Mashima, Y., Tanaka, Y., 1998. Transforming growth factorbeta 1 promotes contraction of collagen gel by bovine corneal fibroblasts through differentiation of myofibroblasts. Invest. Ophthalmol. Vis. Sci. 39, 699–704.

［ ９ ］Schiro, J.A., Chan, B.M., Roswit, W.T., Kassner, P.D., Pentland, A.P., Hemler, M.E., Eisen, A.Z., Kupper, T.S., 1991. Integrin alpha 2 beta 1 （VLA-2） mediates reorganization and contraction of collagen matrices by human cells. Cell 67, 403–410.

［１０］Davis, G.E., Pintar Allen, K.A., Salazar, R., Maxwell, S.A., 2001. Matrix metalloproteinase-1 and -9 activation by plasmin regulates a novel endothelial cell-mediated mechanism of collagen gel contraction and capillary tube regression in three-dimensional collagen matrices. J. Cell Sci. 114, 917–930.

［１１］Margulis, A., Nocka, K.H., Wood, N.L., Wolf, S.F., Goldman, S.J., Kasaian, M.T., 2009. MMP dependence of fibroblast contraction and collagen production induced by human mast cell activation in a threedimensional collagen lattice. Am. J. Physiol. Lung Cell. Mol. Physiol. 296, L236–L247.

［１２］Phillips, J.A., Bonassar, L.J., 2005. Matrix metalloproteinase activity synergizes with alpha2beta1 integrins to enhance collagen remodeling. Exp. Cell Res. 310, 79–87.

［１３］Pins, G.D., Collins-Pavao, M.E., Van De Water, L., Yarmush, M.L., Morgan, J.R., 2000. Plasmin triggers rapid contraction and degradation of fibroblast-populated collagen lattices. J. Invest. Dermatol. 114, 647–653.

［１４］Scott, K.A., Wood, E.J., Karran, E.H., 1998. A matrix metalloproteinase inhibitor which prevents fibroblast-mediated collagen lattice contraction. FEBS Lett. 441, 137–140.

［１５］East, L., Isacke, C.M., 2002. The mannose receptor family. Biochim. Biophys. Acta 1572, 364–386.

［１６］Isacke, C.M., van der Geer, P., Hunter, T., Trowbridge, I.S., 1990. p180, a novel recycling transmembrane glycoprotein with restricted cell type expression. Mol. Cell Biol. 10, 2606–2618.

［１７］Sheikh, H., Yarwood, H., Ashworth, A., Isacke, C.M., 2000. Endo180, an endocytic recycling glycoprotein related to the macrophage mannose receptor is expressed on fibroblasts, endothelial cells and macrophages and functions as a lectin receptor. J. Cell Sci. 113, 1021–1032.

［１８］Behrendt, N., Jensen, O.N., Engelholm, L.H., Mortz, E., Mann, M., Dano, K., 2000. A urokinase receptor-associated protein with specific collagen binding properties. J. Biol. Chem. 275, 1993–2002.

［１９］Engelholm, L.H., Ingvarsen, S., Jurgensen, H.J., Hillig, T., Madsen, D.H., Nielsen, B.S., Behrendt, N., 2009. The collagen receptor uPARAP/Endo180. Front. Biosci. 14, 2103–2114.

［２０］Engelholm, L.H., List, K., Netzel-Arnett, S., Cukierman, E., Mitola, D.J., Aaronson, H., Kjoller, L., Larsen, J.K., Yamada, K.M., Strickland, D.K., Holmbeck, K., Dano, K., Birkedal-Hansen, H., Behrendt, N., Bugge, T.H., 2003. uPARAP/Endo180 is essential for cellular uptake of collagen and promotes fibroblast collagen adhesion. J. Cell Biol. 160, 1009–1015.

［２１］Thomas, E.K., Nakamura, M., Wienke, D., Isacke, C.M., Pozzi, A., Liang, P., 2005. Endo180 binds to the C-terminal region of type I collagen. J. Biol. Chem. 280, 22596–22605.

［２２］Engelholm, L.H., Nielsen, B.S., Dano, K., Behrendt, N., 2001. The urokinase receptor associated protein （uPARAP/endo180）: a novel internalization receptor connected to the plasminogen activation system. Trends Cardiovasc. Med. 11, 7–13.

［２３］Santoro, S.A., Zutter, M.M., 1995. The alpha 2 beta 1 integrin: a collagen receptor on platelets and other cells. Thromb. Haemost. 74, 813–821.

［２４］Zeltz, C., Gullberg, D., 2016. The integrin-collagen connection—a glue for tissue repair? J. Cell Sci. 129, 1284.

［２５］Klein, C.E., Dressel, D., Steinmayer, T., Mauch, C., Eckes, B., Krieg, T., Bankert, R.B., Weber, L., 1991. Integrin alpha 2 beta 1 is upregulated in fibroblasts and highly aggressive melanoma cells in threedimensional collagen lattices and mediates the reorganization of collagen I fibrils. J. Cell Biol. 115, 1427–1436.

［２６］Tian, B., Lessan, K., Kahm, J., Kleidon, J., Henke, C., 2002. Beta 1 integrin regulates fibroblast viability during collagen matrix contraction through a phosphatidylinositol 3-kinase/Akt/protein kinase B signaling pathway. J. Biol. Chem. 277, 24667–24675.

［２７］Sugioka, K., Kodama, A., Yoshida, K., Okada, K., Mishima, H., Aomatsu, K., Matsuo, O., Shimomura, Y., 2014. The roles of urokinase-type plasminogen activator in leukocyte infiltration and inflammatory responses in mice corneas treated with lipopolysaccharide. Invest. Ophthalmol. Vis. Sci. 55, 5338–5350.

［２８］Mishima, H., Okamoto, J., Nakamura, M., Wada, Y., Otori, T., 1998. Collagenolytic activity of keratocytes cultured in a collagen matrix. Jpn. J. Ophthalmol. 42, 79–84.

［２９］Garrett, Q., Khaw, P.T., Blalock, T.D., Schultz, G.S., Grotendorst, G.R., Daniels, J.T., 2004. Involvement of CTGF in TGF-beta1-stimulation of myofibroblast differentiation and collagen matrix contraction in the presence of mechanical stress. Invest. Ophthalmol. Vis. Sci. 45, 1109–1116.

［３０］Tanaka, T., 2000. Comparison of stromal remodeling and keratocyte response after corneal incision and photorefractive keratectomy. Jpn. J. Ophthalmol. 44, 579–590.

［３１］Behrendt, N., 2004. The urokinase receptor （uPAR） and the uPAR-associated protein （uPARAP/ Endo180）: membrane proteins engaged in matrix turnover during tissue remodeling. Biol. Chem. 385, 103–136.

［３２］Madsen, D.H., Engelholm, L.H., Ingvarsen, S., Hillig, T., Wagenaar-Miller, R.A., Kjoller, L., Gardsvoll, H., Hoyer-Hansen, G., Holmbeck, K., Bugge, T.H., Behrendt, N., 2007. Extracellular collagenases and the endocytic receptor, urokinase plasminogen activator receptor-associated protein/Endo180, cooperate in fibroblast-mediated collagen degradation. J. Biol. Chem. 282, 27037–27045.

［３３］Carver, W., Molano, I., Reaves, T.A., Borg, T.K., Terracio, L., 1995. Role of the alpha 1 beta 1 integrin complex in collagen gel contraction in vitro by fibroblasts. J. Cell. Physiol. 165, 425–437.

［３４］Cooke, M.E., Sakai, T., Mosher, D.F., 2000. Contraction of collagen matrices mediated by alpha2beta1A and alpha（v）beta3 integrins. J. Cell Sci. 113, 2375–2383.

［３５］Gullberg, D., Tingstrom, A., Thuresson, A.C., Olsson, L., Terracio, L., Borg, T.K., Rubin, K., 1990. Beta 1 integrin-mediated collagen gel contraction is stimulated by PDGF. Exp. Eye Res. 186, 264–272.

［３６］Wu, C., Dedhar, S., 2001. Integrin-linked kinase （ILK） and its interactors: a new paradigm for the coupling of extracellular matrix to actin cytoskeleton and signaling complexes. J. Cell Biol. 155, 505–510.

［３７］Grinnell, F., 1994. Fibroblasts, myofibroblasts, and wound contraction. J. Cell Biol. 124, 401–404.

［３８］Hinz, B., McCulloch, C.A., Coelho, N.M., 2019. Mechanical regulation of myofibroblast phenoconversion and collagen contraction. Exp. Cell Res. 379, 119–128.

［３９］Jester, J.V., Petroll, W.M., Cavanagh, H.D., 1999. Corneal stromal wound healing in refractive surgery: the role of myofibroblasts. Prog. Retin. Eye Res. 18, 311–356.

［４０］Honardoust, H.A., Jiang, G., Koivisto, L., Wienke, D., Isacke, C.M., Larjava, H., Hakkinen, L., 2006. Expression of Endo180 is spatially and temporally regulated during wound healing. Histopathology 49, 634–648.

［４１］Wienke, D., Davies, G.C., Johnson, D.A., Sturge, J., Lambros, M.B., Savage, K., Elsheikh, S.E., Green, A.R., Ellis, I.O., Robertson, D., Reis-Filho, J.S., Isacke, C.M., 2007. The collagen receptor Endo180 （CD280） is expressed on basal-like breast tumor cells and promotes tumor growth in vivo. Cancer Res. 67, 10230–10240.

［４２］Bundesmann, M.M., Wagner, T.E., Chow, Y.H., Altemeier, W.A., Steinbach, T., Schnapp L.M., 2012. Role of urokinase plasminogen activator receptor-associated protein in mouse lung. Am. J. Respir. Cell Mol. Biol. 46, 233–239.

［４３］Jones, C., Ehrlich, H.P., 2011. Fibroblast expression of alpha-smooth muscle actin, alpha2beta1 integrin and alphavbeta3 integrin: influence of surface rigidity. Exp. Mol. Pathol. 91, 394–399.

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