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Acknowledgements
We thank Ms. Kumiyo Kato for her assistance in this study. This work was
supported by JSPS KAKENHI grant Nos. JP20H02914 (Koji.K.),
JP21K19085 (R.N.), JP20K06528 (Keisuke.K), and JP17H06434 and
JP22H04916 (J.-R.S.), JST-Mirai Program Grant Number JPMJMI20G5
(K.Y.), Takeda Science Foundation (Koji.K.), and the Cyclic Innovation for
Clinical Empowerment (CiCLE) from the Japan Agency for Medical
Research and Development, AMED (T.H., Keisuke.K., K.Y.).
Author contributions
R.N. conceived the project; R.N., N.T., and S.S. prepared the PSI-IsiA
supercomplex and analyzed its biochemical characterization; S.E. performed transcription analysis of IsiAs; Y.U., M.F., and S.A. performed TRF
measurements of the PSI-IsiA supercomplex and their data analysis; T.S.
and N.D. identified subunits in the PSI-IsiA supercomplex; T.H. collected
cryo-EM images; Koji.K. processed the cryo-EM data and reconstructed
the final cryo-EM map; Koji.K. built the structural model and refined the
final model; Y.N. analyzed structural data; Keisuke.K. commented on the
structural data; K.Y. and J.-R.S. supervised this project; R.N. and S.A.
wrote the draft manuscript; and R.N., S.A., and J.-R.S. wrote the final
manuscript, and all of the authors joined the discussion of the results.
Competing interests
The authors declare no competing interests.
Additional information
Supplementary information The online version contains
supplementary material available at
https://doi.org/10.1038/s41467-023-36504-1.
Correspondence and requests for materials should be addressed to Ryo
Nagao, Seiji Akimoto, Koji Yonekura or Jian-Ren Shen.
Peer review information Nature Communications thanks Christoph
Gerle, Petar Lambrev and the other, anonymous, reviewer(s) for their
contribution to the peer review of this work. Peer reviewer reports are
available.
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