論文の公開元へVascular endothelial repair and the influence of circulating antiplatelet drugs in a carotid coil model
概要
Background : Clinicians may choose to administer antiplatelet medications to patients with cerebral aneurysms following endovascular coiling to prevent thrombus formation and vascular occlusion, if they fear a thrombus will form on the platinum wire where it diverges into the vessel from the aneurysm sac. However, the mechanism by which vascular endothelial cells repair a vessel in the living body in the event of a coil deviation and the effects of antiplatelet drugs on these cells have not been fully elucidated. We aimed to investigate the association between endothelial progenitor cells（EPCs）and endothelium formation at the surface of the platinum coils deployed in the carotid artery of rats, and to determine the effects of different antiplatelet drugs on this process.
Subjects and Methods : We established an experimental model using normal and diabetic rats at 12 months of age. The diabetic rats were assigned to four different diet groups, distinguished by whether they were fed plain rat feed, or the same feed supplemented by one of three antiplatelet drugs（cilostazol, aspirin, or clopidogrel: all 0.1%）for 2 weeks, and the carotid artery was perforated by an embolization coil（“carotid coil model”）. We monitored the process by which vascular endothelial cells formed the new endothelium on the surface of the coil by sampling and evaluating the region at 1, 2, and 4 weeks after placement. This repair process was also compared among three groups treated with different antiplatelet drugs（i.e. aspirin, clopidogrel, and cilostazol）. One-way analysis of variance tests were performed to evaluate the differences in vascular thickness between groups, and p<0.05 was considered statistically significant.
Results : The diabetic rats showed delayed neoendothelialization and marked intimal hyperplasia. Cilostazol and clopidogrel effectively counteracted this delayed endothelial repair process. Flk1 immunostaining revealed greater expression in the diabetic rats administered cilostazol, second only to normal rats, suggesting that this agent acted to recruit EPCs.
Conclusion : Neoendothelialization is delayed when vascular endothelial cells fail to function normally, which consequently leads to the formation of hyperplastic tissue. Cilostazol may remedy this dysfunction by recruiting EPCs to the site of injury.
