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大学・研究所にある論文を検索できる 「Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

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Metabolic engineering for 4-aminophenylalanine production from lignocellulosic biomass by recombinant Escherichia coli

Kawaguchi, Hideo Masuo, Shunsuke Wakai, Keiko Takaya, Naoki Hasunuma, Tomohisa Kaneko, Tatsuo Okada, Satoshi Sazuka, Takashi Ogino, Chiaki Kondo, Akihiko 神戸大学

2023.05.27

概要

To synthesize a high-performance biopolyimide bioplastic from lignocellulosic feedstock, Escherichia coli was metabolically engineered to produce 4-amino-L-phenylalanine (4APhe) as a diamine monomer. A high-biomass sorghum cultivar was used as the model lignocellulosic feedstock, and the enzymatic hydrolysate was used as a substrate for 4APhe production in fed-batch culture. When using the ldh mutant strain, HKE6027, 4APhe production from glucose was increased by over three-fold compared to the parent strain. This increase was due to the disruption of biosynthetic pathways that produce either acetate or lactate as by-products. Comparative metabolomic analysis revealed increased flux in both the pentose phosphate and shikimate pathways in HKE6027, resulting in the highest yields. However, 5.7 g L⁻¹ of 4APhe was produced from enzymatic hydrolysis by HKE6027, which was 24% lower than that obtained from glucose. The concentrations of 14 potential fermentation inhibitors present in the enzymatic hydrolysate were determined, and their inhibitory effects on both cell growth and 4APhe production were examined. The addition of groups of potential inhibitors present in the enzymatic hydrolysate of sorghum bagasse showed that benzaldehyde- and cinnamic acid derivatives inhibited 4APhe fermentation at low concentrations (half-maximum inhibitor concentration IC50 = 16 mg L⁻¹), whereas furfural and 5-hydroxymethylfurfural, which are well-known fermentation inhibitors, inhibited 4APhe fermentation at relatively high concentrations (IC50 = 640 mg L⁻¹). These results provide insight into the design of metabolic pathways tailored for the utilization of lignocellulosic biomass to produce aromatic compounds through the shikimate pathway.

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参考文献

22

1 H. Kawaguchi, K. Takada, T. Elkasaby, R. Pangestu,

M. Toyoshima, P. Kahar, C. Ogino, T. Kaneko and

A. Kondo, Bioresour. Technol., 2022, 344, 126165.

2 S. A. Park, H. Jeon, H. Kim, S. H. Shin, S. Choy, D. S. Hwang,

J. M. Koo, J. Jegal, S. Y. Hwang, J. Park and D. X. Oh, Nat.

Commun., 2019, 10, 2601.

3 R. Y. Getor, N. Mishra and A. Ramudhin, Resour., Conserv.

Recycl., 2020, 163, 105094.

4 B. Gupta, N. Revagade and J. Hilborn, Prog. Polym. Sci., 2007,

32, 455–482.

5 H. Kawaguchi, T. Hasunuma, C. Ogino and A. Kondo, Curr.

Opin. Biotechnol., 2016, 42, 30–39.

6 J. He, N. Magarvey, M. Piraee and L. C. Vining, Microbiology,

2001, 147, 2817–2829.

7 V. Blanc, P. Gil, N. BamasJacques, S. Lorenzon, M. Zagorec,

J. Schleuniger, D. Bisch, F. Blanche, L. Debussche,

J. Crouzet and D. Thibaut, Mol. Microbiol., 1997, 23, 191–202.

8 T. Kaneko, M. A. Ali, I. Captain, P. Perlin and T. J. Deming,

Polym. Chem., 2018, 9, 3466–3472.

9 T. Hirayama, A. Kumar, K. Takada and T. Kaneko, ACS

Omega, 2020, 5, 2187–2195.

10 S. K. Bhatia, S. S. Jagtap, A. A. Bedekar, R. K. Bhatia,

A. K. Patel, D. Pant, J. Rajesh Banu, C. V. Rao, Y. G. Kim

and Y. H. Yang, Bioresour. Technol., 2020, 300, 122724.

11 Y. Suo, W. Li, L. Wan, L. Luo, S. Liu, S. Qin and J. Wang, Appl.

Microbiol. Biotechnol., 2023, 107, 327–339.

12 H. Kawaguchi, H. Teramura, K. Uematsu, K. Y. Hara,

T. Hasunuma, K. Hirano, T. Sazuka, H. Kitano, Y. Tsuge,

P. Kahar, S. Niimi-Nakamura, K. I. Oinuma, N. Takaya,

S. Kasuga, C. Ogino and A. Kondo, Bioresour. Technol.,

2015, 182, 169–178.

13 S. Masuo, S. M. Zhou, T. Kaneko and N. Takaya, Sci. Rep.,

2016, 6, 25764.

14 S. Tateyama, S. Masuo, P. Suvannasara, Y. Oka, A. Miyazato,

K. Yasaki, T. Teerawatananond, N. Muangsin, S. M. Zhou,

23

© 2023 The Author(s). Published by the Royal Society of Chemistry

24

25

26

27

28

29

30

31

32

33

34

35

36

Y. Kawasaki, L. B. Zhu, Z. M. Zhou, N. Takaya and

T. Kaneko, Macromolecules, 2016, 49, 3336–3342.

H. Minakawa, S. Masuo, T. Kaneko and N. Takaya, Process

Biochem., 2019, 77, 100–105.

S. Masuo, Y. Tsuda, T. Namai, H. Minakawa, R. Shigemoto

and N. Takaya, Chembiochem, 2019, 21, 353–359.

J. Sambrook and D. W. Russell, Molecular Cloning:

a Laboratory Manual, Cold Spring Harbor Laboratory Press,

New York, 3rd edn, 2001.

S. Hashimoto, T. Wake, H. Nakamura, M. Minamiyama,

S. Araki-Nakamura, K. Ohmae-Shinohara, E. Koketsu,

S. Okamura, K. Miura, H. Kawaguchi, S. Kasuga and

T. Sazuka, Sci. Rep., 2021, 11, 4532.

K. A. Datsenko and B. L. Wanner, Proc. Natl. Acad. Sci. U. S.

A., 2000, 97, 6640–6645.

H. Kawaguchi, M. Sasaki, A. A. Vertes, M. Inui and

H. Yukawa, Appl. Microbiol. Biotechnol., 2008, 77, 1053–1062.

T. Baba, T. Ara, M. Hasegawa, Y. Takai, Y. Okumura,

M. Baba, K. A. Datsenko, M. Tomita, B. L. Wanner and

H. Mori, Mol. Syst. Biol., 2006, 2, 0008.

J. M. Buescher, S. Moco, U. Sauer and N. Zamboni, Anal.

Chem., 2010, 82, 4403–4412.

H. Kato, Y. Izumi, T. Hasunuma, F. Matsuda and A. Kondo, J.

Biosci. Bioeng., 2012, 113, 665–673.

H. Kawaguchi, Y. Katsuyama, D. Danyao, P. Kahar,

S. Nakamura-Tsuruta, H. Teramura, K. Wakai,

K. Yoshihara, H. Minami, C. Ogino, Y. Ohnishi and

A. Kondo, Appl. Microbiol. Biotechnol., 2017, 101, 5279–5290.

J. R. M. Almeida, T. Modig, A. Petersson, B. Hahn-Hagerdal,

G. Liden and M. F. Gorwa-Grauslund, J. Chem. Technol.

Biotechnol., 2007, 82, 340–349.

G. Padmapriya, V. Dhivya, M. Vishal, Y. A. J. Roshni, Y. Akila

and S. Ramalingam, J. Environ. Biol., 2021, 42, 1239–1248.

T. B. Causey, K. T. Shanmugam, L. P. Yomano and

L. O. Ingram, Proc. Natl. Acad. Sci. U. S. A., 2004, 101,

2235–2240.

T. Kubota, A. Watanabe, M. Suda, T. Kogure, K. Hiraga and

M. Inui, Metab. Eng., 2016, 38, 322–330.

S. Noda, T. Shirai, S. Oyama and A. Kondo, Metab. Eng., 2016,

33, 119–129.

A. Matsuyama, H. Yamamoto and E. Nakano, J. Bacteriol.,

1989, 171, 577–580.

X. Liu, J. Lin, H. Hu, B. Zhou and B. Zhu, World J. Microbiol.

Biotechnol., 2014, 30, 2543–2550.

C. Dev, S. B. Jilani and S. S. Yazdani, Microb. Cell Fact., 2022,

21, 154.

A. L. Luhe, C. Y. Lim, H. Gerken, J. Wu and H. Zhao,

Biotechnol. Appl. Biochem., 2015, 62, 32–36.

T. Mitani, K. Ota, N. Inaba, K. Kishida and H. A. Koyama,

Biol. Pharm. Bull., 2018, 41, 208–212.

H. Kawaguchi, K. Uematsu, C. Ogino, H. Teramura, S. NiimiNakamura, Y. Tsuge, T. Hasunuma, K. Oinuma, N. Takaya

and A. Kondo, Biochem. Eng. J., 2014, 88, 188–194.

A. Feh´

er, C. Feher, M. Rozbach and Z. Barta, Chem. Biochem.

Eng. Q., 2017, 31, 77–87.

RSC Sustainability, 2023, 1, 1043–1054 | 1053

View Article Online

RSC Sustainability

40 X. Wang, L. P. Yomano, J. Y. Lee, S. W. York, H. B. Zheng,

M. T. Mullinnix, K. T. Shanmugam and L. O. Ingram, Proc.

Natl. Acad. Sci. U. S. A., 2013, 110, 4021–4026.

41 J. R. Bowen and T. Kosuge, Plant Physiol., 1979, 64, 382–386.

42 C. G. Friedrich, B. Friedrich and H. G. Schlegel, J. Bacteriol.,

1976, 126, 723–732.

43 K. Takada, T. Noda, T. Kobayashi, T. Harimoto, M. Singh and

T. Kaneko, Polym. J., 2021, 53, 1223–1230.

Open Access Article. Published on 27 May 2023. Downloaded on 4/25/2024 12:45:48 AM.

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

37 K. Takada, H. Shinagawa, Y. Morita, M. S. Grewal, K. Taya,

A. Kumar and T. Kaneko, Chin. J. Polym. Sci., 2020, 38,

1117–1123.

38 D. K. Platt, Engineering and High Performance Plastics Market

Report: A Rapra Market Report, Rapra Technolgy Limit,

Shropshire, UK, 2003.

39 R. P. Babu, K. O'Connor and R. Seeram, Prog. Biomater.,

2013, 2, 8.

Paper

1054 | RSC Sustainability, 2023, 1, 1043–1054

© 2023 The Author(s). Published by the Royal Society of Chemistry

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