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大学・研究所にある論文を検索できる 「Genotyping of a gene cluster for production of colibactin and in vitro genotoxicity analysis of Escherichia coli strains obtained from the Japan Collection of Microorganisms」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。
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Genotyping of a gene cluster for production of colibactin and in vitro genotoxicity analysis of Escherichia coli strains obtained from the Japan Collection of Microorganisms

Kawanishi Masanobu Shimohara Chiaki Oda Yoshimitsu Hisatomi Yuuta Tsunematsu Yuta Sato Michio Hirayama Yuichiro Miyoshi Noriyuki Iwashita Yuji Yoshikawa Yuko Sugimura Haruhiko Mutoh Michihiro Ishikawa Hideki Wakabayashi Keiji Yagi Takashi Watanabe Kenji 大阪府立大学

2020.05.12

概要

Introduction: Colibactin is a small genotoxic molecule produced by enteric bacteria, including certain Escherichia coli (E. coli) strains harbored in the human large intestine. This polyketide-peptide genotoxin is considered to contribute to the development of colorectal cancer. The colibactin-producing (clb+) microorganisms possess a 54- kilobase genomic island (clb gene cluster). In the present study, to assess the distribution of the clb gene cluster, genotyping analysis was carried out among E. coli strains randomly chosen from the Japan Collection of Microorganisms, RIKEN BRC, Japan.

Findings: The analysis revealed that two of six strains possessed a clb gene cluster. These clb+ strains JCM5263 and JCM5491 induced genotoxicity in in vitro micronucleus (MN) tests using rodent CHO AA8 cells. Since the induction level of MN by JCM5263 was high, a bacterial umu test was carried out with a cell extract of the strain, revealing that the extract had SOS-inducing potency in the umu tester bacterium.

Conclusion: These results support the observations that the clb gene cluster is widely distributed in nature and clb+ E. coli having genotoxic potencies is not rare among microorganisms.

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参考文献

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