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になり，その半数は分娩後 3-5 週目に子宮内膜炎に移行
すると示している．つまり，分娩後 3 週目までは子宮内
に細菌が存在することは一般的であり，それ以降に多くの
ウシでは細菌が排除され，そうでないウシは子宮内膜炎に
罹患するといえる．したがって，Gly 群の方が Cnt 群よ
り子宮内からの細菌の排出が早く，その後，子宮内膜炎へ
罹患するウシが少なかった可能性がある．しかし子宮修復
の評価には，本試験で実施したメトリチェック以外にも直
腸検査（LeBlanc ら 2002）や超音波画像診断装置（Scully
ら 2013；Kawashima ら 2018）を用いた子宮角や子宮
頸部の直径評価，細胞診（Barlund ら 2008）および血
中プロスタグランジン F2αの代謝物である 13，14- ジヒ
ドロ -15- ケトプロスタグランジン F2α濃度のモニタリン
グ（Mishra と Prakash 2005；Kawashima ら 2018）
などの様々な方法がある．そのため，乾乳期のグリセリン
給与による子宮修復への効果については，これらの評価方
法を取り入れた更なる調査が必要といえる．
以上より，乾乳牛に対する少量のグリセリンを含むペ
レットの分娩予定 3 週前から分娩までの給与は，給与期
間中の血中 BHBA 濃度の減少や分娩に向けた RFS 低下
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The effect of the glycerol pellet supply on
dairy cows during the close-up dry period
Chiho KAWASHIMA1, Moeri KONDO1, Rui HASEGAWA1, Masaharu AZEGAMI1, Juri OI1, Toshiki
SHIBUYA1, Yuka SUGIMOTO1 and Norio YAMAGISHI2
Field Center of Animal Science and Agriculture, Obihiro University of Agriculture and Veterinary
Medicine，Obihiro, Hokkaido 080-8555, Japan
Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine，
Obihiro, Hokkaido 080-8555, Japan
Corresponding : Chiho KAWASHIMA (fax : ＋81 (0) 155-49-5653, e-mail : kawasima@obihiro.ac.jp)
This study examined the effects of the glycerol pellet supply during the close-up dry period in dairy
cows. The peripartum metabolic status, postpartum disease, milk yield, and recovery of reproductive
function were examined in 41 dry Holstein cows. Daily doses of 200 g of glycerol pellets containing 40 %
glycerol (Gly group, n＝20) or 200 g of placebo pellets without glycerol (Cnt group, n＝21) were orally
administered 21 days before the expected calving date through parturition. The rumen fill score (RFS)
before calving did not change in the Gly group, but the RFS decreased gradually as the calving day
approached (P＜0.05) in the Cnt group. In the Gly group, the β-hydroxybutyrate acid (BHBA) blood
concentration during 2 weeks prepartum was lower (P＜0.05), and the body condition score (BCS)
during 2 weeks prepartum (P＜0.05) and 4 weeks postpartum (P＝0.06) was higher. In addition, blood
non-esterified fatty acid concentration during 4 weeks postpartum was tended to be higher (P＝0.09)
and blood γ-glutamyl transpeptidase activity at 4 and 4.5 week postpartum was higher (P＜0.05) in Gly
group than in Cnt group. The number of cows that developed ketosis after parturition did not differ
between groups, although the Gly group cows with ketosis tended to have lower blood BHBA
concentrations (P＝0.07). There were no differences in the milk yield and recovery of reproductive
function after parturition between groups. Administering 40 % glycerol pellets to dairy cows during the
close-up dry period may help to maintain feed intake, improve hepatic lipidosis, proper maintaining of
BCS, and alleviate ketosis symptoms.
Nihon Chikusan Gakkaiho 92 (2), 159-168, 2021
Key words : dry cow, glycerol pellet, ketosis, β-Hydroxybutyrate acid
日畜会報 92（2）：159-168, 2021
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