リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

大学・研究所にある論文を検索できる 「Osteogenic cocktail induces calcifications in human breast cancer cell line via placental alkaline phosphatase expression.」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。
リケラボ

コピーが完了しました

URLをコピーしました

論文の公開元へ論文の公開元へ
書き出し

Osteogenic cocktail induces calcifications in human breast cancer cell line via placental alkaline phosphatase expression.

伏見, 淳 東京慈恵会医科大学 DOI:info:doi/10.1038/s41598-020-69622-7

2021.10.22

概要

Breast cancer is frequently characterized by calcifications in mammography. The mechanism for calcifications in breast cancer is not completely known. Understanding this mechanism will improve diagnostic accuracy. Herein, we demonstrated that calcifications occur and that alkaline phosphatase enzyme activity increases in MDA-MB-231 cells cultured using an osteogenic cocktail-containing medium. Microarray transcript analysis showed that the PI3K-Akt signaling pathway was significantly involved, with recruitment of placental alkaline phosphatase. Calcifications and alkaline phosphatase enzyme activity were suppressed by silencing placental alkaline phosphatase using a small interfering RNA. Inhibition of the PI3K-Akt signaling pathway suppressed phospho-c-Jun and placental alkaline phosphatase and resulted in absence of calcifications. These findings reveal that breast cancer cells acquire alkaline phosphatase enzyme activity via placental alkaline phosphatase expression and suggest that breast calcification formation is closely associated with the PI3K-Akt signaling pathway.

論文の公開元へ

関連論文

関連論文をもっと見る

参考文献

1. Independent UKPoBCS. The benefits and harms of breast cancer screening: an independent review. Lancet (London, England) 380, 1778–1786 (2012).

2. Gold, R. H., Bassett, L. W. & Widoff, B. E. Highlights from the history of mammography. Radiographics 10, 1111–1131 (1990).

3. Perry, N. et al. European guidelines for quality assurance in breast cancer screening and diagnosis. Fourth edition–summary document. Ann. Oncol. 19, 614–622 (2008).

4. Committee to Revise Mammography Guidelines e. The guidelines for mammography. 3rd ed. IGAKU-SHOIN Ltd, (2010).

5. Cox, R. F. & Morgan, M. P. Microcalcifications in breast cancer: Lessons from physiological mineralization. Bone 53, 437–450 (2013).

6. Giachelli, C. M. Vascular calcification mechanisms. J. Am. Soc. Nephrol. 15, 2959–2964 (2004).

7. Cox, R. F. et al. Microcalcifications in breast cancer: novel insights into the molecular mechanism and functional consequence of mammary mineralisation. Br. J. Cancer 106, 525–537 (2012).

8. Shu, R., McMullen, R., Baumann, M. J. & McCabe, L. R. Hydroxyapatite accelerates differentiation and suppresses growth of MC3T3-E1 osteoblasts. J. Biomed. Mater. Res. A 67, 1196–1204 (2003).

9. Scimeca, M. et al. Microcalcifications in breast cancer: an active phenomenon mediated by epithelial cells with mesenchymal characteristics. BMC Cancer 14, 286 (2014).

10. Sharma, U., Pal, D. & Prasad, R. Alkaline phosphatase: an overview. Indian J. Clin. Biochem. 29, 269–278 (2014).

11. Zanconato, F. et al. Genome-wide association between YAP/TAZ/TEAD and AP-1 at enhancers drives oncogenic growth. Nat. Cell Biol. 17, 1218–1227 (2015).

12. Langenbach, F. & Handschel, J. Effects of dexamethasone, ascorbic acid and beta-glycerophosphate on the osteogenic differentia- tion of stem cells in vitro. Stem Cell Res. Therapy 4, 117 (2013).

13. Park, J. B. The effects of dexamethasone, ascorbic acid, and beta-glycerophosphate on osteoblastic differentiation by regulating estrogen receptor and osteopontin expression. J. Surg. Res. 173, 99–104 (2012).

14. Lammers, L. et al. Impact of DAG stimulation on mineral synthesis, mineral structure and osteogenic differentiation of human cord blood stem cells. Stem Cell Res. 8, 193–205 (2012).

15. Choi, H. D., Noh, W. C., Park, J. W., Lee, J. M. & Suh, J. Y. Analysis of gene expression during mineralization of cultured human periodontal ligament cells. J. Periodontal Implant Sci. 41, 30–43 (2011).

16. Jaiswal, N., Haynesworth, S. E., Caplan, A. I. & Bruder, S. P. Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J. Cell. Biochem. 64, 295–312 (1997).

17. Hamidouche, Z. et al. FHL2 mediates dexamethasone-induced mesenchymal cell differentiation into osteoblasts by activating Wnt/beta-catenin signaling-dependent Runx2 expression. FASEB J. 22, 3813–3822 (2008).

18. Xiao, G. et al. Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells. J. Bone Miner. Res. 17, 101–110 (2002).

19. Dang, D., Prasad, H. & Rao, R. Secretory pathway Ca(2+)-ATPases promote in vitro microcalcifications in breast cancer cells. Mol. Carcinog. 56, 2474–2485 (2017).

20. Moulin, P. et al. Hypophosphatasia may lead to bone fragility: don’t miss it. Eur. J. Pediatr. 168, 783–788 (2009).

21. Whyte, M. P. Physiological role of alkaline phosphatase explored in hypophosphatasia. Ann. N. Y. Acad. Sci 1192, 190–200 (2010).

22. Owen, T. A. et al. Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J. Cell. Physiol. 143, 420–430 (1990).

23. Chen, J., Shapiro, H. S. & Sodek, J. Development expression of bone sialoprotein mRNA in rat mineralized connective tissues. J. Bone Miner. Res. 7, 987–997 (1992).

24. Sharma, T., Radosevich, J. A., Pachori, G. & Mandal, C. C. A molecular view of pathological microcalcification in breast cancer. J. Mamm. Gland Biol. Neoplasia 21, 25–40 (2016).

25. O’Grady, S. & Morgan, M. P. Deposition of calcium in an in vitro model of human breast tumour calcification reveals functional role for ALP activity, altered expression of osteogenic genes and dysregulation of the TRPM7 ion channel. Sci. Rep. 9, 542 (2019).

26. Wada, H. G., Shindelman, J. E., Ortmeyer, A. E. & Sussman, H. H. Demonstration of placental alkaline phosphatase in human breast cancer. Int. J. Cancer 23, 781–787 (1979).

27. Chang, T. C. et al. Regulation of the expression of alkaline phosphatase in a human breast-cancer cell line. Biochem. J. 303(Pt 1), 199–205 (1994).

28. Lukey, M. J., Greene, K. S., Erickson, J. W., Wilson, K. F. & Cerione, R. A. The oncogenic transcription factor c-Jun regulates glutaminase expression and sensitizes cells to glutaminase-targeted therapy. Nat. Commun. 7, 11321 (2016).

29. Network, T. C. G. A. Comprehensive molecular portraits of human breast tumours. Nature 490, 61–70 (2012).

30. Proudfoot, D. et al. Apoptosis regulates human vascular calcification in vitro: evidence for initiation of vascular calcification by apoptotic bodies. Circ. Res. 87, 1055–1062 (2000).

31. Collett, G. D. et al. Axl/phosphatidylinositol 3-kinase signaling inhibits mineral deposition by vascular smooth muscle cells. Circ. Res. 100, 502–509 (2007).

32. Clarke, M. C. et al. Chronic apoptosis of vascular smooth muscle cells accelerates atherosclerosis and promotes calcification and medial degeneration. Circ. Res. 102, 1529–1538 (2008).

33. Ponnusamy, A. et al. FTI-277 inhibits smooth muscle cell calcification by up-regulating PI3K/Akt signaling and inhibiting apop- tosis. PLoS ONE 13, e0196232 (2018).

34. da Huang, W., Sherman, B. T. & Lempicki, R. A. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat. Protoc. 4, 44–57 (2009).

35. da Huang, W., Sherman, B. T. & Lempicki, R. A. Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists. Nucl. Acids Res. 37, 1–13 (2009).

参考文献をもっと見る