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Plant-plant communication via volatile organic compounds in beech

Hagiwara, Tomika 京都大学 DOI:10.14989/doctor.k24657

2023.03.23

概要

Plant defense strategies against herbivores and pathogens
Plants cannot move like animals because they are rooted in one place. For that
reason, they must be sensitive to their surroundings and they protect themselves from
natural enemies. When plants are attacked by herbivores and pathogens, they lose their
leaves, which are essential for photosynthesis are damaged. Therefore, plants take
defensive strategies to protect their leaves. Plant defense strategies can be divided into
two categories: (1) constitutive defense, in which plants always defend themselves, and
(2) induced defense, in which plants defend themselves in response to feeding damage
(Agrawal & Karban 2000). Within each category, they can be further classified into direct
and indirect defense. Constitutive direct defense includes physical defenses such as
trichomes and spines (Agrawal & Spiller 2004) and chemical defenses such as secondary
metabolic products (e.g. tannins, phenols, and alkaloids), which are detested by
herbivores (Baldwin 1989; Steppuhn & Baldwin 2007). In constitutive indirect defense,
plants support natural enemies of their herbivore by providing alternative food or shelter
(Quek et al. 2004). In induced direct defense, constitutive direct defense traits are
enhanced by feeding damage. ...

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127

Appendix

Appendix

1.Operophtera brumata

2.Callitera lunulata

ナミスジフユナミシャク

アカヒゲドクガ

3.Erannis golda

4.Syntypistis punctatella

チャバネフユエダシャク

ブナアオシャチホコ

5.Gall midge

6. Gall midge

Diptera: Cecidomyiidae

Diptera: Cecidomyiidae

ブナハマルタマバエ

128

Appendix

7.Calliteara pseudabietis

8.Actias aliena

リンゴドクガ

オオミズアオ

9.Sphrageidus similis

10.Pathogen damage

モンシロドクガ

11.

Species no. 1 to 5 were found in Kawatabi, Species no. 6 to 10 were found in

Tomakomai, species no.1, 3, 8 and 11 were found in Ashiu, no.8 was found in

Hiruzen.

129

Appendix

Samples of leaves damage in Ashiu.

Sample of clipped leaves.

130

Acknowledgements

Acknowledgements

First of all, I would like to thank my supervisor, associate professor Masae Ishihara,

for providing me with many opportunities to challenge the latest and interesting themes,

as shown in this dissertation. Next, I would like to thank professor Naoko Tokuchi for

providing me with many advices for my research. I would like to thank professor Yuji Isagi

and professor Kaoru Kitajima for providing me with valuable suggestions.

I also appreciate professor Kaori Shiojiri for providing me with valuable advice and for

supporting the studies conducted in the Tomakomai Experimental Forest, Hokkaido

University and the Kawatabi Field Center, Tohoku University, professor Junji

Takabayashi for providing me with a lot of advice and encouraging me to do interesting

research, professor Tsutom Hiura for supporting my survey in Tomakomai Experimental

Forest and providing me with many suggestions, professor Naoki Agetsuma for

supporting my survey regardless of the COVID 19 in the Tomakomai Experimental

Forest, professor Yoshihisa Suyama for helping DNA analysis and the survey in the

Kawatabi Field Center, assistant professor Ayumi Matsuo for helping DNA analysis, Dr.

Daiki Takahashi for helping with my fieldwork in the Kawatabi Field Center.

I also appreciate professor Ryunosuke Tateno and associate professor Hisashi

Hasegawa for providing me with many advices and suggestions, and assistant professor

Michimasa Yamasaki for helping statistical analysis, assistant professor Shunsuke

Matsuoka and Dr. Masanori Ohnishi and Dr. Masataka Nakayama for helping my survey.

I am deeply grateful to professor Richard Karban who helped with the grammatical

editing of the manuscript and my survey in the Ashiu Forest Research Stations, Kyoto

131

Acknowledgements

University. I thank Dr. Rika Ozawa for providing valuable suggestions for detecting VOCs,

and also thank assistant professor Makoto Kashima for helping my study with RNA

analysis and encouraging me to do interesting themes. I also thank professor Junji Sano,

Syogo Fukutomi and Asami Yoneda, for their support during field research in Hiruzen. I

appreciate Junji Hosomi, Megumu Konno, Hiroaki Fujii and all staff of the Ashiu Forest

Research Stations, the Kamigamo Experimental Station and the Kitashirakawa

Experimental Station Field Science and Education Center, Kyoto University for their

support during fieldwork. I also appreciate Dr. Soyoka Makino, Dr. May Thet Su Kyaw

Tint and Akane Kawasaki and the laboratory members for many comments and

suggestions. Finally, I appreciate my family for being remarkably supportive.

This research was financially supported by JSPS KAKENHI Grant Number JP

21J15074, 18H03952, 22H05722, by the Sumitomo Foundation Fiscal 2019 Grant Basic

Science research projects, Grant/Award Number: 190876, and by the Research Institute

for Food and Agriculture of Ryukoku University Grant Number FA1906.

132

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