Effects of High-Frequency Near-Infrared Diode Laser Irradiation on IL-1β-Induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes

概要

࿨ชસชགྷ༁

Effects of High-Frequency Near-Infrared Diode Laser Irradiation
on IL-1β-Induced Expression of Inflammatory Cytokines and
Matrix Metalloproteinases in Human Primary Chondrocytes
ʤϐφॵେക཈ೊࠐࡋ๖Ͷ͕͜ΖԎ঳੓γ΢φΩ΢ϱ͕Γ;ϜφϨ
ρέηϟνϫϕϫτΠʖκ͹ IL-1β ༢಍੓൅‫ݳ‬ͶଲͤΖ߶बഀۛ੼
֐ൔ಍ରϪʖδʖলࣻ͹Ӫ‫ڻ‬ʥ

Ph.D. Applicant Shuzo Sakata

Graduate School of Biomedical and Health Sciences
Hiroshima University

Supervisor: Kotaro Tanimoto
2020

Contents
ᶙ.

Introduction

ᶚ.

Materials and Methods
2-1. Cell Culture
2-2. Chondrocyte human recombinant IL-1β stimulation
2-3. Laser irradiation
2-4. Quantitative real-time polymerase chain reaction analysis
2-5. Western blot analysis
2-6. Enzyme-linked immunosorbent assay (ELISA)
2-7. Immunocytochemistry
2-8. Imaging cytometry analysis
2-9. Electrophoretic mobility shift assay (EMSA)
2-10. NF-κB p65 transcription factor assay
2-11. Statistical analysis

ᶛ.

Results
3-1. Effects of high-frequency near-infrared diode laser
irradiation on gene expression of inflammatory cytokines
in NHAC-Kn cells
3-2. Effects of high-frequency near-infrared diode laser

irradiation on gene expression of MMPs in NHAC-Kn
cells
3-3. Effects of IL-1β or high-frequency near-infrared diode
laser irradiation on inflammatory cytokines protein
expression in NHAC-Kn cells
3-4. Effects of high-frequency near-infrared diode laser
irradiation on protein expression of matrix
metalloproteinases in NHAC-Kn cells
3-5. Effects of high-frequency near-infrared diode laser
irradiation on secretion of matrix metalloproteinases in
NHAC-Kn cells
3-6. Effect of IL-1β or high-frequency near-infrared diode laser
irradiation on NF-κB phosphorylation
3-7. Effect of IL-1β or high-frequency near-infrared diode laser
irradiation on nuclear NF-κB translocation
3-8. Effect of IL-1β or high-frequency near-infrared diode laser
irradiation on NF-κB transcriptional activity
ᶜ.

Discussion

ᶝ.

Figures and Tables

ᶞ.

References