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Acknowledgements
I would like to appreciate my supervisor, Emeritus Professor Min Yao, who trained in
my bachelor's, master's, and doctoral programs even after her retirement. She respected
my initiative and provided me with various opportunities, allowing me to enjoy science
and acquire expertise in structural biology. She also taught me presentation skills for
various domestic and international conferences and scientific writing skills. I will work
harder in Germany from April 2023 and come back to Japan to repay your kindness.
I would also like to express my gratitude to my supervisor, Professor Toyoyuki Ose.
His useful advice on experimental techniques and discussions at lab meetings have greatly
advanced my research. He taught me not only the theory of crystallography, but also the
importance of applying the best approaches depending on research questions.
I wish to thank Professor Yoshikazu Tanaka (at Tohoku University) for a lot of support
including reviewing our papers. He also gave me an opportunity to enter the Ambitions
Leader’s Program (ALP), which widen my comprehensive perspective, including
QM/MM simulations and MD simulations. I am grateful to my collaborator, Associate
Professor Masaki Horitani (at Saga University) for EPR experiments. When I had trouble
with the results, he always responded sincerely to my questions.
For the establishment of the foundation of the TtuA project, I would like to express my
respect and gratitude to my predecessors, Dr. Minghao Chen and Mr. Shun Narai. I have
also learned countless things from them in terms of experimental techniques, analysis
methods, and even their research attitude. I would also like to thank Associate Professor
Koji Kato (at Okayama University) and Specially Appointed Associate Professor Jian Yu
(at Osaka University) for teaching me how to use various software for X-ray
crystallography. I also thank the beamline staff at the Photon Factory and SPring-8 for
their support. This work was supported by the Japan Society for the Promotion of Science
(JSPS), ALP, and the Nitobe School at Hokkaido University.
Thanks to the support of all members of my laboratory and friends, I was able to
continue my research and successfully complete my doctoral program. Finally, I would
like to thank my family, especially my parents, with all my heart for their continuous
dedication and support throughout the past 27 years.
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