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Figures

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Fig. 1 Acanthamoeba castellanii medusavirus J1 (ACMV-J1) replication and its particle feature

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[12]. (a) ACMV-J1 replication in amoeba cell after infection. (b) A cryo-EM image of ACMV-J1.

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Scale 200 nm. (c) A 3D reconstruction of ACMV-J1 virion. Scale 50 nm.

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Fig 2 Maximum-likelihood phylogenetic tree of Nucleocytoviricota. The tree was based on a

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concatenated amino acid sequence alignment of seven marker genes constructed using MAFFT

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(v.7.471) and trimAl (v.1.4.1) and was built using IQ-TREE 2 (v.2.1.3) [15–17]. The model was

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LG+F+R8 selected by the built-in Modelfinder of IQ-TREE 2 [18]. The branch supports were

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computed by 1000 ultrafast bootstrap and SH-aLRT [19]. The tree was visualized by iTOL,

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the round labels on branches represent high confidence supports with Ultrafast bootstrap ≥

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95%, SH-aLRT ≥ 80%. Position of proposed family “Mamonoviridae” is reported in red

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background and marked with stars.

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Fig. 3 Boxplots for (a) tip distance, (b) ANI, (c) TETRA, and (d) normalized OGs sharing level.

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The horizontal black line represents the value between clandestinovirus and Acanthamoeba

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castellanii medusavirus J1 (ACMV-J1), a member of proposed species “Medusavirus

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medusae”.

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