Cellular characterization of hiPS CMs cultured on PMEA analogous polymers with different bound water content

1)

Percentage of

displacement

behavior (5 μm~)

(%)

Mean

displacement

with pulsation

(μm)

0.05

0.08

0.21

0.98

0.395

0.287

0.393

0.488

PET

PMEMA

PEEA

PMEA

4.

Conclusions

In this study, hiPS-CMs were cultured on

PMEA analogues and the effect on hiPS-CMs

was investigated. Substrates were prepared by

coating biocompatible materials, PMEA

analogues, on PET. The morphology of

hiPS-CMs

single

cells

was

spherical

morphology and the adhesion area was smaller,

as the high content of binding water in the

scaffold.

The

self-pulsating

hiPS-CMs

aggregates on the fifth day of culture were

then evaluated. The results showed that the

area of self-pulsating cell aggregates tended to

be smaller on substrates with higher amounts

of bound water. Furthermore, the magnitude

of displacement associated with their

self-pulsating

was

larger.

Especially,

hiPS-CMs cultured on PMEA showed

significantly larger contraction behavior than

others. This result suggests that the bound

water content of scaffolds affects cell adhesion

and

weakens

cell-scaffold

interaction,

resulting in stronger intercellular binding of

cell aggregates and better self-contraction. In

previous studies, cell alignment of hiPS-CMs

with nanofiber scaffolds has been successful in

expressing many genes related to intercellular

junctions.23) There was also an example of a

study that succeeded in improving pulsation

force by geometrically aggregating the

hiPS-CMs by using Faraday waves.22) This

study demonstrated that hiPS-CMs exhibit

different cell aggregation behaviors and

different

cellular

characteristics

when

cultured on PMEA analogues with different

amounts of bound water. In the future, it is

expected to establish a scaffold material that

can improve the pulsatility of hiPS-CMs by

controlling the amount of bound water on the

scaffold surface.

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