Aravin AA, Hannon GJ, Brennecke J. 2007. The Piwi-piRNA pathway provides an adaptive defense in the transposon arm race. Science 318: 761-764.
Brennecke J, Aravin AA, Stark A, Dus M, Kellis M, Sachidanandam R, Hannon GJ. 2007. Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. Cell 128: 1089-1103.
Carmell MA, Girard A, Kant HJG, Bourc'his D, Bestor TH, Rooij DG, Hannon GJ. 2007. MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline. Dev Cell 12: 503-514.
Conti E, Uy M, Leighton L, Blobel G, Kuriyan J. 1998. Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor Karyopherin α. Cell 94: 193-204.
Cox DN, Chao A, Baker J, Chang L, Qiao D, Lin H. 1998. A novel class of evolutionarily conserved genes defined by piwi are essential for stem cell self-renewal. Genes Dev 12: 3715-3727.
Czech B, Preall JB, McGinn J, Hannon GJ. 2013. A transcriptome-wide RNAi screen in the Drosophila ovary reveals factors of the germline piRNA pathway. Mol Cell 50: 749-761.
Dönertas D, Sienski G, Brennecke J. 2013. Drosophila Gtsf1 is an essential component of the Piwi-mediated transcriptional silencing complex. Genes Dev 27: 1693-1705.
Elkayam E, Kuhn C-D, Tocilj A, Haase AD, Greene EM, Hannon GJ, Joshua-Tor L. 2012. The structure of human Argonaute-2 in complex with miR-20a. Cell 150: 100-110.
Eulalio A, Huntzinger E, Izaurralde E. 2008. GW182 Interaction with Argonaute Is Essential for miRNA-Mediated Translational Repression and mRNA Decay. Nat Struct Mol Biol 15: 346−353.
Fontes MR, Teh T, Toth G, John A, Pavo I, Jans DA, Kobe B. 2003. Role of flanking sequences and phosphorylation in the recognition of the simian-virus-40 large T-antigen nuclear localization sequences by importin-alpha. Biochem J 375: 339-349.
Ghildiyal M, Zamore PD. 2009. Small silencing RNAs: an expanding universe. Nat Rev Genetics 10: 94-108.
Girard A, Sachidanandam R, Hannon GJ and Carmell MA. 2006. A germline-specific class of small RNAs binds mammalian Piwi proteins. Nature 442: 199-202.
Goldfarb DS, Corbett AH, Mason DA, Harreman MT, Adam SA. 2004. Importina: a multipurpose nuclear-transport receptor. Trends Cell Biol 14: 505-514.
Görlich D, Prehn S, Laskey RA and Hartmann E. 1994. Isolation of a protein that is essential for the first step of nuclear protein import. Cell 79: 767-778.
Görlich D. Transport into and out of the cell nucleus. 1998. EMBO J 17: 2721-2727.
Grimson A, Srivastava M, Fahey B, Woodcroft BJ, Chiang HR, King N, Degnan BM, Rokhsar DS, Bartel DP. 2008. Early origins and evolution of microRNAs and PIWI-interacting RNAs in animals. Nature 455:1193-1197.
Gunawardane LS, Saito K, Nishida KM, Miyoshi K, Kawamura Y, Nagami T, Siomi H, Siomi MC. 2007. A slicer-mediated mechanism for repeat-associated siRNA 5' end formation in Drosophila. Science 315:1587-1590.
Handler D, Meixner K, Pizka M, Lauss K, Schmied C, Gruber FS, Brennecke J. 2013. The genetic makeup of the Drosophila piRNA pathway. Mol Cell 50: 762-777.
Ishizu H, Siomi H, Siomi MC. 2012. Biology of PIWI-interacting RNAs: new insights into biogenesis and function inside and outside of germlines. Genes Dev 26: 2361-2373.
Iwasaki S, Kawamata T, Tomari Y. 2009. Drosophila Argonaute1 and Argonaute2 Employ Distinct Mechanisms for Translational Repression. Mol Cell 34: 58−67.
Iwasaki YW, Siomi MC, Siomi H. 2015. PIWI-interacting RNA: Its biogenesis and functions. Annu Rev Biochem 84: 405-433.
Iwasaki YW, Murano K, Ishizu H, Shibuya A, Iyoda Y, Siomi MC, Siomi H, Saito K. 2016. Piwi modulates chromatin accessibility by regulating multiple factors including histone H1 to repress transposons. Mol Cell 63: 408-419.
Juliano C, Wang J, Lin H. 2011. Uniting germline and stem cells: the function of Piwi proteins and the piRNA pathway in diverse organisms. Annu Rev Genet 45: 447-469.
Kalderon D, Richardson WD, Markham AF, Smith AE. 1984. Sequence requirements for nuclear location of simian virus 40 large-T antigen. Nature 311: 33-38.
Kalmykova AI, Nurminsky DI, Ryzhov DV, Shevelyov YY. 2005. Regulated chromatin domain comprising cluster of co-expressed genes in Drosophila melanogaster. Nucleic Acids Res 33: 1435-1444.
Kawamura Y, Saito K, Kin T, Ono Y, Asai K, Sunohara T, Okada TN, Siomi MC, Siomi H. 2008. Drosophila endogenous small RNAs bind to Argonaute 2 in somatic cells. Nature 453: 793-797.
Khurana JS, Theurkauf W. 2010. piRNAs, transposon silencing, and Drosophila germline development. J Cell Biol 191: 905-913.
Klenov MS, Sokolova OA, Yakushev EY, Stolyarenko AD, Mikhaleva EA,
Lavrov SA, Gvozdev VA. 2011. Separation of stem cell maintenance and transposon silencing functions of Piwi protein. Proc Natl Acad Sci USA 108: 18760-18765.
Kobe B. 1999. Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin α. Nat Struct Mol Biol 6: 388-397.
Kussel P, Frasch M. 1995. Pendulin, a Drosophila protein with cell cycle-dependent nuclear localization, is required for normal cell proliferation. J Cell Biol 129: 1491-1507.
Le Thomas A, Rogers AK, Webster A, Marinov GK, Liao SE, Perkins EM, Hur JK, Aravin AA, Tóth KF. 2013. Piwi induces piRNA-guided transcriptional silencing and establishment of a repressive chromatin state. Genes Dev 27: 390-399.
Li C, Vagin VV, Lee S, Xu J, Ma S, Xi H, Seitz H, Horwich MD, Syrzycka M, Honda BM, Kittler EL, Zapp ML, Klattenhoff C, Schulz N, Theukauf WE, Weng Z, Zamore PD. 2009. Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in flies. Cell 137: 509-521.
Lippai M, Tilian L, Baros I, Mihaly J, Erdelyi M, Belecz I, Mathe E, Posfai J, Nagy A, Udvardy A, et al. 2000. The Ketel gene encodes a Drosophila homologue of importin-β. Genetics. 156: 1889-1900.
Livak KJ. 1990. Detailed structure of the Drosophila melanogaster stellate genes and their transcripts. Genetics 124: 303-316.
Malone CD, Hannon GJ. 2009. Small RNAs as guardians of the genome. Cell 136: 656-668.
Malone CD, Brennecke J, Dus M, Stark A, McCombie WR, Sachidanandam R, Hannon GJ. 2009. Specialized piRNA Pathways Act in Germline and Somatic Tissues of the Drosophila Ovary. Cell 137:522−535.
Mason DM, Fleming RJ, Goldfarb DS. 2002. Drosophila melanogaster importin α1 and α3 can replace importin α2 during spermatogenesis but not oogenesis. Genetics 161: 157-170.
Mason DM, Mathe E, Fleming RJ, Goldfarb DS. 2003. The Drosophila melanogaster importin α3 locus encodes an essential gene required for the development of both larval and adult tissues. Genetics 165: 1943-1958.
Mason DM, Stage DE, Goldfarb DS. 2009. Evolution of the metazoan-specific importin α gene family. J Mol Evol 68: 351-365.
Matsumoto N, Sato K, Nishimasu H, Namba Y, Miyakubi K, Dohmae N, Ishitani R, Siomi H, Siomi MC, Nureki O. 2015. Crystal structure and activity of the endoribonuclease domain of the piRNA pathway factor Maelstrom. Cell Rep 11: 366-375.
Matsumoto N, Nishimasu H, Sakakibara K, Nishida KM, Hirano T, Ishitani R, Siomi H, Siomi MC, Nureki O. 2016. Crystal structure of silkworm PIWI-clade Argonaute Siwi bound to piRNA. Cell 167: 484-497.
Miyoshi K, Tsukumo H, Nagami T, Siomi H, Siomi MC. 2005. Slicer function of Drosophila Argonautes and its involvement in RISC formation. Genes Dev 19: 2837-2848.
Muerdter F, Guzzardo PF, Gillis J, Luo Y, Yu Y, Chen C, Fekete R, Hannon GJ. 2013. A genome-wide RNAi screen draws a genetic framework for transposon control and primary piRNA biogenesis in Drosophila. Mol Cell 50: 736-748.
Murota Y, Ishizu H, Nakagawa S, Iwasaki YW, Shibata S, Kamatani MK, Saito K, Okano H, Siomi H, Siomi MC. 2014. Yb integrates piRNA intermediates and processing factors into perinuclear bodies to enhance piRISC assembly. Cell Rep 8: 103-113.
Nagao A, Sato K, Nishida KM, Siomi H, Siomi MC. 2010. Gender-specific hierarchy in nuage localization of PIWI-interacting RNA factors in Drosophila. Front Genet 2: 1-9.
Nishida KM, Saito K, Mori T, Kawamura Y, Nagami-Okada T, Inagaki S, Siomi H, Siomi MC. 2007. Gene silencing mechanisms mediated by Aubergine-piRNA complexes in Drosophila male gonad. RNA 13:1911-1922.
Nishida KM, Iwasaki YW, Murota Y, Nagao A, Mannen T, Kato Y, Siomi H, Siomi MC. 2015. Respective functions of two distinct Siwi complexes assembled during PIWI-interacting RNA biogenesis in Bombyx germ cells. Cell Rep 10: 193-203.
Nishida KM, Sakakibara K, Iwasaki YW, Yamada H, Murakami R, Kawamura T, Komada T, Siomi H, Siomi MC. 2018. Hierarchical roles of mitochondrial Papi and Zucchini in Bombyx germline piRNA biogenesis. Nature 555: 260-264.
Ohtani H, Iwasaki YW, Shibuya A, Siomi H, Siomi MC, Saito, K. 2013. DmGTSF1 is necessary for Piwi–piRISC-mediated transcriptional transposon silencing in the Drosophila ovary. Genes Dev 27: 1656-1661.
Olivieri D, Sykora MM, Sachidanandam R, Mechtler K, Brennecke J. 2010. An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila. EMBO J 29: 3301-3317.
Phadnis N, Hsieh E, Malik HS. 2011. Birth, death, and replacement of karyopherins in Drosophila. Mol Biol Evol 5: 1429-1440.
Pumroy RA, Cingolani G. 2015. Diversification of importin-α isoforms in cellular trafficking and disease states. Biochem J 466: 13-28. Ratan R, Mason DA, Sinnot B, Goldfarb DS, Fleming RJ. 2008. Drosophila importin α1 performs paralog-specific functions essential for gametogenesis. Genetics 178: 839-350.
Riggleman B, Wieschaus E, Schedl P. 1989. Molecular analysis of the armadillo locus: uniformly distributed transcripts and a protein with novel internal repeats are associated with a Drosophila segment polarity gene. Genes Dev 3: 96-113.
Robbins J, Dilworth SM, Laskey RA, Dingwall, C. 1991. Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence. Cell 64:615-623.
Saito K, Nishida KM, Mori T, Kawamura Y, Miyoshi K, Nagami T, Siomi H, Siomi MC. 2006. Specific association of Piwi with rasiRNAs derived from retrotransposon and heterochromatic regions in the Drosophila genome. Genes Dev 20: 2214-2222.
Saito K, Inagaki S, Mituyama T, Kawamura Y, Ono Y, Sakota E, Kotani H, Asai K, Siomi H, Siomi MC. 2009. A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila. Nature 461: 1296-1301.
Saito K, Ishizu H, Komai M, Kotani H, Kawamura Y, Nishida KM, Siomi H, Siomi MC. 2010. Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. Genes Dev 24: 2493-2498.
Sato K, Iwasaki YW, Shibuya A, Carninci P, Tsuchizawa Y, Ishizu H, Siomi MC, Siomi H. 2015. Krimper enforces an antisense bias on piRNA pools by binding AGO3 in the Drosophila germline. Mol Cell 59: 553-563.
Schmidt A, Palumbo G, Bozzetti MP, Tritto P, Pimpinelli S, Schäfer U. 1999. Genetic and molecular characterization of sting, a gene involved in crystal formation and meiotic drive in the male germ line of Drosophila melanogaster. Genetics 151: 749-760.
Sienski G, Dönertas D, Brennecke J. 2012. Transcriptional silencing of transposons by Piwi and Maelstrom and its impact on chromatin state and gene expression. Cell 151: 964-980.
Sienski G, Batki J, Senti KA, Dönertas D, Tirian L, Meixner K, Brennecke J. 2015. Silencio/CG9754 connects the Piwi-piRNA complex to the cellular heterochromatin machinery. Genes Dev 29: 2258-2271.
Siomi MC, Sato K, Pezic C, Aravin AA. 2011. PIWI-interacting small RNAs: the vanguard of genome defence. Nat Rev Mol Cell Biol 12: 246-258.
Stewart M. 2007. Molecular mechanism of the nuclear protein import cycle. Nat Rev Mol Cell Biol 8: 195-208.
Sumiyoshi T, Sato K, Yamamoto H, Iwasaki YW, Siomi H, Siomi MC. 2016. Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells. Genes Dev 30: 1617-1622.
Török I, Strand D, Schmitt R, Tick G, Török T, Kiss I, Mechler B. 1995. The overgrown hematopoietic organs-31 tumor suppressor gene of Drosophila encodes an Importin-like protein accumulating in the nucleus at the onset of mitosis. J Cell Biol 129: 1473-1489.
Vagin VV, Sigova A, Li C, Seitz H, Gvozdev V, Zamore PD. 2006. A Distinct Small RNA Pathway Silences Selfish Genetic Elements in the Germline. Science 313: 320−324.
Watanabe T, Totoki Y, Toyoda A, Kaneda M, Kuramochi-Miyagawa S, Obata Y, Chiba H, Kohara Y, Kono T, Nakano T, et al. 2008. Endogenous siRNAs from Naturally Formed dsRNAs Regulate Transcripts in Mouse Oocytes. Nature 453: 539−543.
Yamashiro H, Siomi MC. 2018. Piwi-interacting RNA in Drosophila: Biogenesis, Transposon Regulation, and Beyond. Chem. Rev. 118: 4404-4421.
Yu Y, Gu J, Jin Y, Luo Y, Preall JB, Ma J, Czech B, Hannon GJ. 2015. Panoramix enforces piRNA-dependent cotranscriptional silencing. Science 350: 339-342.