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https://doi.org/10.1038/s41467-023-36408-0
Acknowledgements
Additional information
We thank Chieko Matsuda, Masumi Sanada, Hiroki Hagizawa, and Yuya
Nishijima for their assistance and helpful discussion. We thank the iPS
Cell Research Fund for its research support. This study was supported by
JSPS KAKENHI Grant No. 18H02923 (to N.T.) and WPI Premium Research
Institute for Human Metaverse Medicine (PRIMe) (to N.T.) from the Japan
Society for the Promotion of Science. This study was also supported by
the Center for Clinical Application Research on Specific Disease/
Organ (type B) Grant No. 21bm0304004h0009 (to N.T.); Research
Project for Practical Applications of Regenerative Medicine Grant No.
21bk0104079h0003 (to N.T.); Practical Research Project for Rare/
Intractable Diseases (step 1) Grant No. 21ek0109452h0002 (to N.T.);
Core Center for iPS Cell Research Grant No. 20bm0104001h0008 (to
N.T.); and the Acceleration Program for Intractable Diseases Research
utilizing disease-specific iPS cells Grant No. 20bm0804006h0004
(to N.T.) from the Japan Agency for Medical Research and Development (AMED).
Supplementary information The online version contains
supplementary material available at
https://doi.org/10.1038/s41467-023-36408-0.
Author contributions
K.A., S.M., and N.T. designed experiments. K.O. prepared cyiPSCs. A.Y.
created the cyiPS-cart. K.A. transplanted cyiPS-Carts into monkeys. K.A.
performed CT and histological analyses. K.A., M.M., S.K., and N.T. performed scRNA-seq analysis. N.H. and Y.T. performed experiments
regarding Sik3. K.A. and N.T. wrote the manuscript.
Competing interests
N.T. is an inventor and Kyoto University is a holder of the patent on
“An efficient chondrocyte induction method” (PCT/JP2014/079117).
This patent is licensed to Asahi KASEI corporation. Y.T. is an
employee of Asahi KASEI. The remaining authors declare no
competing interests.
Nature Communications | (2023)14:804
Correspondence and requests for materials should be addressed to
Noriyuki Tsumaki.
Peer review information Nature Communications thanks Denis
Evseenko and the other, anonymous, reviewer(s) for their contribution to
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