リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

大学・研究所にある論文を検索できる 「Characterization of Dclk1 isoforms in the developing mouse brain」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

コピーが完了しました

URLをコピーしました

論文の公開元へ論文の公開元へ
書き出し

Characterization of Dclk1 isoforms in the developing mouse brain

ベルゴリオ, エミリア 東京大学 DOI:10.15083/0002006711

2023.03.24

概要

論文審査の結果の要旨
氏名

ベルゴリオ

エミリア

本論文は主に3部(序章、結果、考察)からなる。序章では、本研究の主たる研究対
象であるDCLK1(Doublecortin-like kinase 1)遺伝子のゲノム構造およびスプライシング
様式について解説し、さらにDCLK1遺伝子の変異と精神神経疾患との関連、各DCLK1ア
イソフォームに関するこれまでの知見が記載されている。
結果の章では、まずマウス大脳皮質の発生過程に着目し、スプライシングにより
DCLK1遺伝子から生み出される4つの異なるアイソフォームであるDCLK1-L、DCL、
CPG16、CARPの発現量がどのよう変化するのかについて検討を行った。具体的には、次
世代シークエンサーにより無作為に解読されたマウス大脳皮質の遺伝子情報を利用し
て、各DCLK1アイソフォームをコードする遺伝子配列に特徴的なギャップシークエンス
のリード数を算出することにより、各アイソフォームの発現レベルを定量的に解析し
た。その結果、DCLK1-L遺伝子は胎生期から発現しその後も安定して発現し続けるこ
と、DCLは胎生期に強い発現が認められるが出生直前に急激に発現が低下すること、逆
にCPG16は出生後に急激な発現上昇が見られることを示した。更に、特異的抗体を用い
たウェスタンブロッティングにより、この各アイソフォームの転写産物の発現量変動
は、各転写産物がコードするタンパク質の発現量変動と概して一致することを示した。
次に、in situハイブリダイゼーション法を用いて、マウス大脳皮質における各DCLK1
アイソフォームの発現分布について解析を行った。具体的には、各DCLK1アイソフォー
ムをコードするmRNAに対して特異的に結合するプローブを設計することが不可能なの
で、2つのアイソフォームに対して結合しうるプローブを4つ設計し、その組み合わせ
から、個々のアイソフォームの発現分布を類推することを試みた。その結果、出生後の
マウス大脳皮質では、DCLK1-Lが比較的下層部に局在するのに対してCPG16は上層部に
分布することを見出した。更に興味深いことに、海馬領域では各アイソフォームの分布
が大きく異なり、DCLK1-Lは海馬全体に発現が見られるのに対して、DCLは歯状回、
CPG16はCA1-3領域に局在していることを発見した。
次に、胎児期のマウス大脳皮質ニューロンに各アイソフォームを過剰発現させること
により、in vivoレベルの機能推定を行った。その結果、DCLK1-Lアイソフォームの過剰
発現は新生ニューロンの大脳皮質層移動に異常をもたらすが、他の3つのアイソフォー
ムの過剰発現では大きな異常は見られないことを発見した。更に、キナーゼ活性を失う
遺伝子変異を導入したDCLK1-Lの過剰発現では層移動に大きな影響が見られないことか
ら、DCLK1-L過剰発現の効果はキナーゼ活性に依存することを示した。最後に、
DCLK1-Lアイソフォームを培養下の大脳皮質ニューロンに過剰発現させたところ、樹状

突起の形態形成に異常をもたらし、更に、その形態異常の誘導能はキナーゼ活性に依存
することを示した。以上の結果から、DCLK1-Lの過剰発現は、何らかの標的分子をリン
酸化することにより樹状突起形成に異常をもたらし、in vivoレベルでは神経細胞の移動
に異常をもたらす可能性が示唆された。
考察の章では、本研究において新たに見出されたDCLK1アイソフォームの発現動態と
大脳皮質内局在のデータセットに基づいて、各アイソフォーム独自の機能、および病態
との関連について議論している。
これらの論文の各章で示された研究成果は、これまで散発的に行われていたDCLK1ア
イソフォームの発現・機能研究を、同一のプラットフォームにおいて同時に行い、はじ
めて統合的なデータセットを示した論文である。したがって、論文提出者の研究成果は
博士(理学)の学位を受けるにふさわしいと判定した。
以上の理由から、博士(理学)の学位を授与できると認める。

この論文で使われている画像

参考文献

Bauman, Tyler M, Emily A Ricke, Sally A Drew, Wei Huang, and William A Ricke (2016).

“Quantitation of Protein Expression and Co-localization Using Multiplexed Immunohistochemical Staining and Multispectral Imaging.” eng. In: J Vis Exp 110. issn: 1940087X (Electronic); 1940-087X (Linking). doi: 10.3791/53837.

Berke, J D, R F Paletzki, G J Aronson, S E Hyman, and C R Gerfen (1998). “A complex

program of striatal gene expression induced by dopaminergic stimulation.” eng. In: J

Neurosci 18.14, pp. 5301–5310. issn: 0270-6474 (Print); 1529-2401 (Electronic); 02706474 (Linking). doi: 10.1523/JNEUROSCI. 18-14-05301.1998.

Bielas, Stephanie L, Finley F Serneo, Magdalena Chechlacz, Thomas J Deerinck, Guy

A Perkins, Patrick B Allen, Mark H Ellisman, and Joseph G Gleeson (2007). “Spinophilin

facilitates dephosphorylation of doublecortin by PP1 to mediate microtubule bundling

at the axonal wrist.” eng. In: Cell 129.3, pp. 579–591. issn: 0092-8674 (Print); 00928674 (Linking). doi: 10.1016/ j.cell.2007.03.023.

Binley, Kate E, Wai S Ng, James R Tribble, Bing Song, and James E Morgan (2014).

“Sholl analysis: a quantitative comparison of semi-automated methods.” eng. In: J

Neurosci Methods 225, pp. 65–70. issn: 1872-678X (Elec- tronic); 0165-0270 (Linking).

doi: 10.1016/j.jneumeth.2014.01.017.

Boekhoorn, Karin, Angela Sarabdjitsingh, Hendrik Kommerie, Karin de Punder, Theo

Schouten, Paul J. Lucassen, and Erno Vreugdenhil (2008). “Doublecortin (DCX) and

doublecortin-like (DCL) are differentially expressed in the early but not late stages of

murine neocortical development.” In: Journal of Comparative Neurology 507.4, pp.

1639–1652. doi: https://doi.org/ 10.1002/cne.21646. eprint:

https://onlinelibrary.wiley.com/doi/ pdf/10.1002/cne.21646. url:

https://onlinelibrary.wiley.com/ doi/abs/10.1002/cne.21646.

Braun, Martin, Robert Kirsten, Niels J Rupp, Holger Moch, Falko Fend, Nicolas

Wernert, Glen Kristiansen, and Sven Perner (2013). “Quantification of protein

expression in cells and cellular subcompartments on immunohistochemical sections

using a computer supported image analysis system.” eng. In: Histol Histopathol 28.5,

pp. 605–610. issn: 1699-5848 (Electronic); 0213- 3911 (Linking). doi: 10.14670/HH28.605.

Burgess, H A and O Reiner (2000). “Doublecortin-like kinase is associated with

microtubules in neuronal growth cones.” eng. In: Mol Cell Neurosci 16.5, pp. 529–541.

issn: 1044-7431 (Print); 1044-7431 (Linking). doi: 10.1006/ mcne.2000.0891.

77

Burgess, Harold A. and Orly Reiner (2002). “Alternative Splice Variants of Doublecortinlike Kinase Are Differentially Expressed and Have Different Kinase Activities.” In:

Journal of Biological Chemistry.

Chhatriya, Bishnupriya, Moumita Mukherjee, Sukanta Ray, Barsha Saha, Som- datta

Lahiri, Sandip Halder, Indranil Ghosh, Sujan Khamrui, Kshaunish Das, Samsiddhi

Bhattacharjee, Saroj Kant Mohapatra, and Srikanta Goswami (2020). “Transcriptome

analysis identifies putative multi-gene signature distinguishing benign and malignant

pancreatic head mass.” In: Journal of Translational Medicine 18.1, p. 420. doi:

10.1186/s12967-020-02597-1. url: https://doi.org/10.1186/s12967-020-02597-1.

Cooper, Jonathan A (2014). “Molecules and mechanisms that regulate multi- polar

migration in the intermediate zone.” eng. In: Front Cell Neurosci 8, p. 386. issn: 16625102 (Print); 1662-5102 (Electronic); 1662-5102 (Link-ing).

doi:10.3389/fncel.2014.00386.

Cregger, Melissa, Aaron J Berger, and David L Rimm (2006). “Immunohistochemistry

and quantitative analysis of protein expression.” eng. In: Arch Pathol Lab Med 130.7,

pp. 1026–1030. issn: 1543-2165 (Electronic); 0003- 9985 (Linking). doi: 10.1043/15432165(2006)130{$[$}1026:IAQAOP{$] $}2.0.CO;2.

Crowe, Alexandra R and Wei Yue (2019). “Semi-quantitative Determination of Protein

Expression using Immunohistochemistry Staining and Analysis: An Integrated

Protocol.” eng. In: Bio Protoc 9.24. issn: 2331-8325 (Print); 2331-8325 (Electronic);

2331-8325 (Linking). doi: 10.21769/BioProtoc. 3465.

Danielson, Nathan B, Patrick Kaifosh, Jeffrey D Zaremba, Matthew Lovett- Barron,

Joseph Tsai, Christine A Denny, Elizabeth M Balough, Alexander R Goldberg, Liam J

Drew, Ren e

́ Hen, Attila Losonczy, and Mazen A Kheirbek (2016). “Distinct Contribution

of Adult-Born Hippocampal Granule Cells to Context Encoding.” eng. In: Neuron 90.1,

pp. 101–112. issn: 1097-4199 (Electronic); 0896-6273 (Print); 0896-6273 (Linking). doi:

10.1016/j. neuron.2016.02.019.

Deuel TA, Liu JS, Corbo JC, Yoo SY, Rorke-Adams LB, Walsh CA. Genetic interactions

between doublecortin and doublecortin-like kinase in neuronal migration and axon

outgrowth. Neuron. 2006 Jan 5;49(1):41-53. doi: 10.1016/j.neuron.2005.10.038. PMID:

16387638.

Engels, Bart M, Theo G Schouten, Joost van Dullemen, Ilse Gosens, and Erno

Vreugdenhil (2004). “Functional differences between two DCLK splice vari- ants.” eng.

In: Brain Res Mol Brain Res 120.2, pp. 103–114. issn: 0169-328X (Print); 0169-328X

(Linking). doi: 10.1016/j.molbrainres.2003.10.006.

Erno Vreugdenhil Nicole Datson, Bart Engels Jeannette de Jong Silvana van

Koningsbruggen Marcel Schaaf E. Ronald de Kloet (1999). “Kainate-elicited seizures

78

induce mRNA encoding a CaMK-related peptide: A putative modulator of kinase

activity in rat hippocampus.” In:

Ferreira, Tiago A, Arne V Blackman, Julia Oyrer, Sriram Jayabal, Andrew J Chung,

Alanna J Watt, P Jesper Sj o

̈ str o

̈ m, and Donald J van Meyel (2014). “Neuronal

morphometry directly from bitmap images.” eng. In: Nat Methods 11.10, pp. 982–984.

issn: 1548-7105 (Electronic); 1548-7091 (Print); 1548- 7091 (Linking). doi:

10.1038/nmeth.3125.

Francis, F, A Koulakoff, D Boucher, P Chafey, B Schaar, M C Vinet, G Friocourt, N

McDonnell, O Reiner, A Kahn, S K McConnell, Y Berwald-Netter, P Denoulet, and J

Chelly (1999). “Doublecortin is a developmentally regulated, microtubule-associated

protein expressed in migrating and differentiating neurons.” eng. In: Neuron 23.2, pp.

247–256. issn: 0896-6273 (Print); 0896- 6273 (Linking). doi: 10.1016/s08966273(00)80777-1.

Garrido-Martin, Diego, Emilio Palumbo, Roderic Guig ́o, and Alessandra Breschi (Aug.

2018). “ggsashimi: Sashimi plot revised for browser- and annotation- independent

splicing visualization.” In: PLOS Computational Biology 14.8, pp. 1–6. doi:

10.1371/journal.pcbi.1006360. url: https://doi. org/10.1371/journal.pcbi.1006360.

Gassmann, Max, Beat Grenacher, Bianca Rohde, and Johannes Vogel (2009).

“Quantifying Western blots: Pitfalls of densitometry.” In: ELECTROPHORE- SIS 30.11,

pp. 1845–1855. doi: https://doi.org/10.1002/elps. 200800720. eprint:

https://onlinelibrary.wiley.com/doi/pdf/10.002/elps.200800720. url:

https://onlinelibrary.wiley.com/ doi/abs/10.1002/elps.200800720.

Gleeson, J G, K M Allen, J W Fox, E D Lamperti, S Berkovic, I Scheffer, E C Cooper, W

B Dobyns, S R Minnerath, M E Ross, and C A Walsh (1998). “Doublecortin, a brainspecific gene mutated in human X-linked lissencephaly and double cortex syndrome,

encodes a putative signaling pro- tein.” eng. In: Cell 92.1, pp. 63–72. issn: 0092-8674

(Print); 0092-8674 (Linking). doi: 10.1016/s0092-8674(00)80899-5.

Gleeson, J G, P T Lin, L A Flanagan, and C A Walsh (1999). “Doublecortin is a

microtubule-associated protein and is expressed widely by migrating neurons.” eng. In:

Neuron 23.2, pp. 257–271. issn: 0896-6273 (Print); 0896- 6273 (Linking). doi:

10.1016/s0896-6273(00)80778-3.

Gottesman, Irving I and Todd D Gould (2003). “The endophenotype concept in

psychiatry: etymology and strategic intentions.” eng. In: Am J Psychiatry 160.4, pp.

636–645. issn: 0002-953X (Print); 0002-953X (Linking). doi: 10.1176/appi.ajp.160.4.636.

Håvik, Bjarte, Franziska A. Degenhardt, Stefan Johansson, Carla P. D. Fernandes,

Anke Hinney, Andre Scherag, Helle Lybæk, Srdjan Djurovic, Andrea Christoforou, Kari

M. Ersland, Sudheer Giddaluru, Michael C. O’Donovan, Michael J. Owen, Nick

79

Craddock, Thomas W. Mu h

̈ leisen, Manuel Mattheisen, Benno G. Schimmelmann,

Tobias Renner, Andreas Warnke, Beate Herpertz- Dahlmann, Judith Sinzig, Ozgur

Albayrak, Marcella Rietschel, Markus M. Nothen, Clive R. Bramham, Thomas Werge,

Johannes Hebebrand, Jan Haavik, Ole A. Andreassen, Sven Cichon, Vidar M. Steen,

and Stephanie Le Hellard (Apr. 2012). “DCLK1 Variants Are Associated across

Schizophrenia and At- tention Deficit/Hyperactivity Disorder.” In: PLOS ONE 7.4, pp.

1–12. doi:10.1371/journal.pone.0035424.

url:https://doi.org/10.1371/journal.pone.0035424.

Hevroni, Dana, Amir Rattner, Marsha Bundman, Doron Lederfein, Awni Gabarah,

Miriam Mangelus, Michael A. Silverman, Hilla Kedar, Cathy Naor, Masayo Kornuc,

Tamar Hanoch, Rony Seger, Lars E. Theill, Elly Nedivi, Gal Richter- Levin, and Yoav

Citri (1998). “Hippocampal plasticity involves extensive gene induction and multiple

cellular mechanisms.” In: Journal of Molecular Neuroscience 10.2, pp. 75–98. doi:

10.1007/BF02737120. url: https: //doi.org/10.1007/BF02737120.

Kaiser, Odett, Pooyan Aliuos, Kirsten Wissel, Thomas Lenarz, Darja Werner, Gu n

̈ ter

Reuter, Andrej Kral, and Athanasia Warnecke (2013). “Dissociated neurons and glial

cells derived from rat inferior colliculi after digestion with papain.” eng. In: PLoS One

8.12, e80490. issn: 1932-6203 (Electronic); 1932-6203 (Linking). doi:

10.1371/journal.pone.0080490.

Katz, Yarden, Eric T Wang, Edoardo M Airoldi, and Christopher B Burge (2010).

“Analysis and design of RNA sequencing experiments for identifying isoform

regulation.” In: Nature Methods 7.12, pp. 1009–1015. doi: 10.1038/nmeth.1528. url:

https://doi.org/10.1038/nmeth.1528.

Kim, Daehwan, Joseph M. Paggi, Chanhee Park, Christopher Bennett, and Steven L.

Salzberg (2019). “Graph-based genome alignment and genotyp- ing with HISAT2 and

HISAT-genotype.” In: Nature Biotechnology 37.8, pp. 907–915. doi: 10.1038/s41587019-0201-4. url: https://doi. org/10.1038/s41587-019-0201-4.

Koizumi, Hiroyuki, Teruyuki Tanaka, and Joseph G Gleeson (2006). “Doublecortin- like

kinase functions with doublecortin to mediate fiber tract decussation and neuronal

migration.” eng. In: Neuron 49.1, pp. 55–66. issn: 0896-6273 (Print); 0896-6273

(Linking). doi: 10.1016/j.neuron.2005.10.040.

Koizumi, Hiroyuki, Hiromi Fujioka, Kazuya Togashi, James Thompson, John R. Yates

III, Joseph G. Gleeson, and Kazuo Emoto (2017). “DCLK1 phosphorylates the

microtubule-associated protein MAP7D1 to promote axon elongation in cortical

neurons.” In: Developmental Neurobiology 77.4, pp. 493– 510. doi:

https://doi.org/10.1002/dneu.22428. eprint: https:

//onlinelibrary.wiley.com/doi/pdf/10.1002/dneu.22428. url:

https://onlinelibrary.wiley.com/doi/abs/10.1002/dneu.22428.

80

Kroon, Tim, Eline van Hugte, Lola van Linge, Huibert D. Mansvelder, and Rhiannon M.

Meredith (2019). “Early postnatal development of pyramidal neurons across layers of

the mouse medial prefrontal cortex.” In: Scientific Reports 9.1, p. 5037. doi:

10.1038/s41598-019-41661-9. url: https: //doi.org/10.1038/s41598-019-41661-9.

Le Hellard, Stéphanie, Bjarte Håvik, Thomas Espeseth, Harald Breilid, Roger Løvlie,

Michelle Luciano, Alan J Gow, Sarah E Harris, John M Starr, Karin Wibrand, Astri J

Lundervold, David J Porteous, Clive R Bramham, Ian J Deary, Ivar Reinvang, and Vidar

M Steen (2009). “Variants in doublecortin- and calmodulin kinase like 1, a gene upregulated by BDNF, are associ- ated with memory and general cognitive abilities.” eng.

In: PLoS One 4.10, e7534. issn: 1932-6203 (Electronic); 1932-6203 (Linking). doi:

10.1371/ journal.pone.0007534.

Li, Heng (Sept. 2011). “A statistical framework for SNP calling, mutation discovery,

association mapping and population genetical parameter estimation from sequencing

data.” In: Bioinformatics 27.21, pp. 2987–2993. issn: 1367-4803. doi:

10.1093/bioinformatics/btr509. eprint: https: //academic.oup.com/bioinformatics/articlepdf/27/21/2987/ 577342/btr509.pdf. url: https://doi.org/10.1093/bioinformatics/ btr509.

Li Y, Shen M, Stockton ME, Zhao X. Hippocampal deficits in neurodevelopmental

disorders. Neurobiol Learn Mem. 2019 Nov;165:106945. doi:

10.1016/j.nlm.2018.10.001. Epub 2018 Oct 12. PMID: 30321651; PMCID:

PMC6461531.

Lin, Peter T., Joseph G. Gleeson, Joseph C. Corbo, Lisa Flanagan, and Christo- pher

A. Walsh (2000). “DCAMKL1 Encodes a Protein Kinase with Homol- ogy to

Doublecortin that Regulates Microtubule Polymerization.” In: Jour- nal of Neuroscience

20.24, pp. 9152–9161. issn: 0270-6474. doi: 10.1523/ JNEUROSCI.20-24-09152.2000.

eprint: https://www.jneurosci.org/ content/20/24/9152.full.pdf. url:

https://www.jneurosci.org/ content/20/24/9152.

Lipka, Joanna, Lukas C Kapitein, Jacek Jaworski, and Casper C Hoogenraad (2016).

“Microtubule-binding protein doublecortin-like kinase 1 (DCLK1) guides kinesin-3mediated cargo transport to dendrites.” eng. In: EMBO J 35.3, pp. 302–318. issn:

1460-2075 (Electronic); 0261-4189 (Print); 0261- 4189 (Linking). doi:

10.15252/embj.201592929.

Longair, Mark H., Dean A. Baker, and J. Douglas Armstrong (July 2011). “Simple

Neurite Tracer: open source software for reconstruction, visualization and analysis of

neuronal processes.” In: Bioinformatics 27.17, pp. 2453– 2454. issn: 1367-4803. doi:

10.1093/bioinformatics/btr390. eprint: https : / / academic . oup . com / bioinformatics

/ article - pdf / 27 / 17/2453/598305/btr390.pdf. url: https://doi.org/10.1093/

bioinformatics/btr390.

81

Longo, Patti A, Jennifer M Kavran, Min-Sung Kim, and Daniel J Leahy (2013).

“Transient mammalian cell transfection with polyethylenimine (PEI).” eng. In: Methods

Enzymol 529, pp. 227–240. issn: 1557-7988 (Electronic); 0076- 6879 (Print); 0076-6879

(Linking). doi: 10.1016/B978-0-12-418687- 3.00018-5.

Lopez-Bigas, Nuria, Benjamin Audit, Christos Ouzounis, Genıs Parra, and Roderic

Guigo (2005). “Are splicing mutations the most frequent cause of hereditary disease?”

eng. In: FEBS Lett 579.9, pp. 1900–1903. issn: 0014-5793 Print); 0014-5793 (Linking).

doi: 10.1016/j.febslet.2005.02.047.

Matsuo, Tomohiko, Tatsuya Hattori, Akari Asaba, Naokazu Inoue, Nobuhiro Kanomata,

Takefumi Kikusui, Reiko Kobayakawa, and Ko Kobayakawa (2015). “Genetic dissection

of pheromone processing reveals main olfactory system- mediated social behaviors in

mice.” eng. In: Proc Natl Acad Sci U S A 112.3, E311–20. issn: 1091-6490 (Electronic);

0027-8424 (Print); 0027- 8424 (Linking). doi: 10.1073/pnas.1416723112.

Nadarajah, B., Brunstrom, J. E., Grutzendler, J., Wong, R. O., Pearlman, A. L. 2001.

Two modes of radial migration in early development of the cerebral cortex. Nat.

Neurosci. 4, 143–150.

Nagamine, Tadashi, Sachiko Shimomura, Noriyuki Sueyoshi, and Isamu Kameshita

(Jan. 2011). “Influence of Ser/Pro-rich domain and kinase domain of double cortin-like

protein kinase on microtubule-binding activity.” In: The Journal of Biochemistry 149.5,

pp. 619–627. issn: 0021-924X. doi: 10.1093/jb/ mvr013. eprint:

https://academic.oup.com/jb/article-pdf/149/ 5/619/6443118/mvr013.pdf. url:

https://doi.org/10.1093/jb/ mvr013.

Noctor, S. C., Martínez-Cerdeño, V., Ivic, L., Kriegstein, A. R. 2004. Cortical neurons

arise in symmetric and asymmetric division zones and migrate through specific phases.

Nat. Neurosci. 7, 136–144.

Omori, Y, M Suzuki, K Ozaki, Y Harada, Y Nakamura, E Takahashi, and T Fujiwara

(1998). “Expression and chromosomal localization of KIAA0369, a putative kinase

structurally related to Doublecortin.” eng. In: J Hum Genet 43.3, pp. 169–177. issn:

1434-5161 (Print); 1434-5161 (Linking). doi: 10. 1007/s100380050063.

Patel, Onisha, Weiwen Dai, Mareike Mentzel, Michael D W Griffin, Juliette Serindoux,

Yoann Gay, Stefanie Fischer, Shoukat Sterle, Ashleigh Kropp, Christopher J Burns,

Matthias Ernst, Michael Buchert, and Isabelle S Lucet (2016). “Biochemical and

Structural Insights into Doublecortin-like Kinase Domain 1.” eng. In: Structure 24.9, pp.

1550–1561. issn: 1878-4186 (Elec- tronic); 0969-2126 (Linking). doi:

10.1016/j.str.2016.07.008.

Pike, Jeremy A, Iain B Styles, Joshua Z Rappoport, and John K Heath (2017).

“Quantifying receptor trafficking and colocalization with confocal microscopy.” eng. In:

82

Methods 115, pp. 42–54. issn: 1095-9130 (Electronic); 1046-2023 (Linking). doi:

10.1016/j.ymeth.2017.01.005.

Portes, V des, F Francis, J M Pinard, I Desguerre, M L Moutard, I Snoeck, L C Meiners,

F Capron, R Cusmai, S Ricci, J Motte, B Echenne, G Ponsot, O Dulac, J Chelly, and C

Beldjord (1998). “doublecortin is the major gene causing X-linked subcortical laminar

heterotopia (SCLH).” eng. In: Hum Mol Genet 7.7, pp. 1063–1070. issn: 0964-6906

(Print); 0964-6906 (Linking). doi: 10.1093/hmg/7.7.1063.

Rathi, Vinay K., Harlan M. Krumholz, Frederick A. Masoudi, and Joseph S. Ross (Aug.

2020). “Postmarket Clinical Evidence for High-Risk Therapeutic Med- ical Devices

Receiving Food and Drug Administration Premarket Approval in 2010 and 2011.” In:

JAMA Network Open 3.8, e2014496–e2014496. issn: 2574-3805. doi:

10.1001/jamanetworkopen.2020.14496. eprint: https:

//jamanetwork.com/journals/jamanetworkopen/articlepdf/2769911/

rathi\_2020\_ld\_200099\_1602699681.74656.pdf. url: https:

//doi.org/10.1001/jamanetworkopen.2020.14496.

Ristanović, Dušan, Nebojša T. Milšević, and Vesna Štulic (2006). “Application of

modified Sholl analysis to neuronal dendritic arborization of the cat spinal cord.” In:

Journal of Neuroscience Methods 158.2, pp. 212 –218. issn: 0165-0270. doi:

https://doi.org/10.1016/j.jneumeth.2006.05. 030. url:

http://www.sciencedirect.com/science/article/pii/ S0165027006002871.

Robinson, Mark D., Davis J. McCarthy, Gordon J. Smyth (2010). “edgeR: a

Bioconductor package for differential expression analysis of digital gene expression

data.” Bioinformatics, 26(1), 139-140. doi: 10.1093/bioinformatics/btp616.

Saaltink, Dirk-Jan, Erik W van Zwet, and Erno Vreugdenhil (2020). “Doublecortin- Like

Is Implicated in Adult Hippocampal Neurogenesis and in Motivational Aspects to

Escape from an Aversive Environment in Male Mice.” eng. In: eNeuro 7.5. issn: 23732822 (Electronic); 2373-2822 (Linking). doi: 10. 1523/ENEURO.0324-19.2020.

Saaltink, Dirk-Jan, Bjarte H a

̊ vik, Carla S Verissimo, P J Lucassen, and Erno

Vreugdenhil (2012). “Doublecortin and doublecortin-like are expressed in overlapping

and non-overlapping neuronal cell population: implications for neurogenesis.” eng. In: J

Comp Neurol 520.13, pp. 2805–2823. issn: 1096- 9861 (Electronic); 0021-9967

(Linking). doi: 10.1002/cne.23144.

Schaar, Bruce T, Kazuhisa Kinoshita, and Susan K McConnell (2004). “Doublecortin

microtubule affinity is regulated by a balance of kinase and phos- phatase activity at

the leading edge of migrating neurons.” eng. In: Neu- ron 41.2, pp. 203–213. issn:

0896-6273 (Print); 0896-6273 (Linking). doi: 10.1016/s0896-6273(03)00843-2.

83

Schenk, Geert J, Bart Engels, Yan-Ping Zhang, Carlos P Fitzsimons, Theo Schouten,

Marieke Kruidering, E Ron de Kloet, and Erno Vreugdenhil (2007). “A potential role for

calcium / calmodulin-dependent protein kinase-related peptide in neuronal apoptosis:

in vivo and in vitro evidence.” eng. In: Eur J Neurosci 26.12, pp. 3411–3420. issn:

1460-9568 (Electronic); 0953-816X (Linking). doi: 10.1111/j.1460-9568.2007.05956.x.

Schenk, Geert J, Barbera Veldhuisen, Olga Wedemeier, Caroline C McGown, Theo G

Schouten, Melly Oitzl, E Ron de Kloet, and Erno Vreugdenhil (2010). “Over-expression

of C-DCLK-short in mouse brain results in a more anxious behavioral phenotype.” eng.

In: Physiol Behav 101.4, pp. 541– 548. issn: 1873-507X (Electronic); 0031-9384

(Linking). doi: 10.1016/ j.physbeh.2010.08.002.

Schenk, Geert J., Erno Vreugdenhil, Chantal J.Y. Hubens, Barbera Veldhuisen, E. Ron

[de Kloet], and Melly S. Oitzl (2011). “Hippocampal CARP over- expression solidifies

consolidation of contextual fear memories.” In: Phys- iology Behavior 102.3, pp. 323 –

331. issn: 0031-9384. doi: https : / / doi.org/10.1016/j.physbeh.2010.11.024. url:

http://www. sciencedirect.com/science/article/pii/S0031938410004245.

Shin, Euikyung, Yutaro Kashiwagi, Toshihiko Kuriu, Hirohide Iwasaki, Teruyuki Tanaka,

Hiroyuki Koizumi, Joseph G. Gleeson, and Shigeo Okabe (2013). “Doublecortin-like

kinase enhances dendritic remodelling and negatively reg- ulates synapse maturation.”

In: Nature Communications 4.1, p. 1440. doi: 10.1038/ncomms2443. url:

https://doi.org/10.1038/ncomms2443.

Shu, Tianzhi, Huang-Chun Tseng, Tamar Sapir, Patrick Stern, Ying Zhou, Ka- mon

Sanada, Andre Fischer, Fr e

́ de

́ ric M. Coquelle, Orly Reiner, and Li-Huei Tsai (2006).

“Doublecortin-like Kinase Controls Neurogenesis by Regulat- ing Mitotic Spindles and

M Phase Progression.” In: Neuron 49.1, pp. 25–39. doi: 10.1016/j.neuron.2005.10.039.

url: https://doi.org/10. 1016/j.neuron.2005.10.039.

Silverman, M A, O Benard, H Jaaro, A Rattner, Y Citri, and R Seger (1999). “CPG16, a

novel protein serine/threonine kinase downstream of cAMP- dependent protein

kinase.” eng. In: J Biol Chem 274.5, pp. 2631–2636. issn: 0021-9258 (Print); 0021-9258

(Linking). doi: 10.1074/jbc.274.5.2631.

Soltesz, Ivan and Attila Losonczy (2018). “CA1 pyramidal cell diversity enabling parallel

information processing in the hippocampus.” eng. In: Nat Neurosci 21.4, pp. 484–493.

issn: 1546-1726 (Electronic); 1097-6256 (Print); 1097- 6256 (Linking). doi:

10.1038/s41593-018-0118-0.

Sossey-Alaoui, Khalid and Anand K. Srivastava (1999). “DCAMKL1, a Brain- Specific

Transmembrane Protein on 13q12.3 That Is Similar to Doublecortin (DCX).” In:

Genomics 56.1, pp. 121 –126. issn: 0888-7543. doi: https://

doi.org/10.1006/geno.1998.5718. url: http://www.sciencedirect.

com/science/article/pii/S0888754398957184.

84

Tabata, H and K Nakajima (2001). “Efficient in utero gene transfer system to the

developing mouse brain using electroporation: visualization of neuronal migration in the

developing cortex.” eng. In: Neuroscience 103.4, pp. 865– 872. issn: 0306-4522 (Print);

0306-4522 (Linking). doi: 10.1016/s0306- 4522(01)00016-1.

Tabata, Hidenori and Kazunori Nakajima (2003). “Multipolar migration: the third mode

of radial neuronal migration in the developing cerebral cortex.” eng. In: J Neurosci

23.31, pp. 9996–10001. issn: 1529-2401 (Electronic); 0270-6474 (Print); 0270-6474

(Linking). doi: 10.1523/JNEUROSCI.23- 31-09996.2003.

Tan, Han Yen and Tuck Wah Ng (2008). “Accurate step wedge calibration for

densitometry of electrophoresis gels.” In: Optics Communications 281.10, pp. 3013 –

3017. issn: 0030-4018. doi: https://doi.org/10.1016/ j.optcom.2008.01.012. url:

http://www.sciencedirect.com/ science/article/pii/S0030401808000461.

Tanaka, Teruyuki, Hiroyuki Koizumi, and Joseph G. Gleeson (July 2006). “The

Doublecortin and Doublecortin-Like Kinase 1 Genes Cooperate in Murine Hippocampal

Development.” In: Cerebral Cortex 16.suppl1, pp. i69–i73. issn: 1047-3211. doi:

10.1093/cercor/bhk005. eprint: https:// academic . oup . com / cercor / article - pdf /

16 / suppl \ _1 / i69 / 17295647/bhk005.pdf. url: https://doi.org/10.1093/cercor/

bhk005.

Taylor, C R and R M Levenson (2006). “Quantification of immunohistochemistry–

issues concerning methods, utility and semiquantitative assessment II.” eng.In:

Histopathology 49.4, pp. 411–424. issn: 0309-0167 (Print); 0309-0167 (Linking). doi:

10.1111/j.1365-2559.2006.02513.x.

Vázquez-Vélez, Gabriel E, Kristyn A Gonzales, Jean-Pierre Revelli, Carolyn J Adamski,

Fatemeh Alavi Naini, Aleksandar Baji c

́ , Evelyn Craigen, Ronald Richman, Sabrina M

Heman-Ackah, Matthew J A Wood, Maxime W C Rousseaux, and Huda Y Zoghbi

(2020). “Doublecortin-like Kinase 1 Regu- lates -Synuclein Levels and Toxicity.” eng. In:

J Neurosci 40.2, pp. 459–477. issn: 1529-2401 (Electronic); 0270-6474 (Print); 02706474 (Linking). doi: 10.1523/JNEUROSCI.1076-19.2019.

Vreugdenhil E, Datson N, Engels B, de Jong J, van Koningsbruggen S, Schaaf M, de

Kloet ER. Kainate-elicited seizures induce mRNA encoding a CaMK-related peptide: a

putative modulator of kinase activity in rat hippocampus. J Neurobiol. 1999

Apr;39(1):41-50. PMID: 10213452.

Vreugdenhil, Erno, Sharon M. Kolk, Karin Boekhoorn, Carlos P. Fitzsimons, Marcel

Schaaf, Theo Schouten, Angela Sarabdjitsingh, Rosana Sibug, and Paul J. Lucassen

(2007). “Doublecortin-like, a microtubule-associated pro- tein expressed in radial glia,

is crucial for neuronal precursor division and ra- dial process stability.” In: European

Journal of Neuroscience 25.3, pp. 635– 648. doi: 10.1111/j.1460-9568.2007.05318.x.

85

eprint: https: //onlinelibrary.wiley.com/doi/pdf/10.1111/j.1460-9568. 2007.05318.x. url:

https://onlinelibrary.wiley.com/doi/abs/ 10.1111/j.1460-9568.2007.05318.x.

Walsh, Thomas A.S. Deuel Judy S. Liu Joseph C. Corbo Seung-Yun Yoo Lucy B.

Rorke-Adams Christopher A. (2006). “Genetic Interactions between Dou- blecortin and

Doublecortin-like Kinase in Neuronal Migration and Axon Out- growth.” In:

Wu, Jing Qin, Xi Wang, Natalie J Beveridge, Paul A Tooney, Rodney J Scott, Vaughan

J Carr, and Murray J Cairns (2012). “Transcriptome sequenc- ing revealed significant

alteration of cortical promoter usage and splicing in schizophrenia.” eng. In: PLoS One

7.4, e36351. issn: 1932-6203 (Elec- tronic); 1932-6203 (Linking). doi:

10.1371/journal.pone.0036351.

Yan, Qinghong, Sebastien M. Weyn-Vanhentenryck, Jie Wu, Steven A. Sloan, Ye

Zhang, Kenian Chen, Jia Qian Wu, Ben A. Barres, and Chaolin Zhang (2015).

“Systematic discovery of regulated and conserved alternative exons in the mammalian

brain reveals NMD modulating chromatin regulators.” In: Proceedings of the National

Academy of Sciences 112.11, pp. 3445– 3450. issn: 0027-8424. doi:

10.1073/pnas.1502849112. eprint: https: //www.pnas.org/content/112/11/3445.full.pdf.

url: https: //www.pnas.org/content/112/11/3445.

Zygmunt, Magdalena, Dz e

̇ sika Hoinkis, Jacek Hajto, Marcin Piechota, Boz e

̇ na

Skupien -́ Rabian, Urszula Jankowska, Sylwia Kedracka-Krok, Jan Rodriguez

Parkitna,andMichal Korostyn ś ki(2018).“Expressionofalternativelyspliced variants of

the Dclk1 gene is regulated by psychotropic drugs.” eng. In: BMC Neurosci 19.1, p. 55.

issn: 1471-2202 (Electronic); 1471-2202 (Linking). doi: 10.1186/s12868-018-0458-4.

86

...

参考文献をもっと見る

全国の大学の
卒論・修論・学位論文

一発検索!

この論文の関連論文を見る