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Acknowledgements
First, I would like to express my deepest gratitude to Professor Yasuyoshi Sakai (Division of Applied Life
Sciences, Graduate School of Agriculture, Kyoto University) for his directions of this study and valuable
discussion during the whole course of graduate school. His ideas always stimulated my scientific interest
and enhanced my ability of logical thinking. I wish to express sincere thanks to Associate Professor
Hiroya Yurimoto (Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University)
for his directions of this study, helpful advice, valuable discussions and continuous support. I am deeply
grateful to Assistant Professor Kosuke Shiraishi (Division of Applied Life Sciences, Graduate School of
Agriculture, Kyoto University), Associate Professor Masahide Oku and Professor Jun Hoseki
(Department of Bioscience and Technology, Faculty of Bioenvironmental Sciences, Kyoto University of
Advanced Science) for technical supports, meaningful discussion and kind advice.
Next, I appreciate Dr. Shinji Ito (Medical Research Support Center, Graduate School of Medicine,
Kyoto University) for the analysis of phosphorylation using LC-MS/MS in chapter I. And I would like to
express my sincere gratitude to Dr. Shin Ohsawa (for chapter I and chapter III), Mr. Taiju Okamoto (for
chapter I), Mr. Gakuto Kitayama (for chapter I), Mr. Kosuke Iwase (for chapter II), and Ms. Nono Saso
(for chapter II) for their experimental supports on my research and meaningful discussions. I would like
to thank Ms. Shiori Katayama (Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto
University) for the technical guidance of microscopy observation and flow cytometry (for preparing the
strains in chapter II). Special thanks are due to Ms. Yuri Fujita and Mr. Ryota Ikeda for their warm
supports to my laboratory life.
Finally, I am grateful to all members of my laboratory, staff, and fellows in the Division of Applied
Life Sciences, Graduate School of Agriculture, Kyoto University. I am glad to spend precious time with
them and will never forget the memories during this course. I wish to express deep appreciation to my
family and my wife Ms. Airi Inoue for their continuous support.
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Publications
1. Inoue, K., Ohsawa, S., Yurimoto, H., and Sakai, Y. (2022)
“Phosphoregulation of the transcription factor Mxr1 plays a crucial role in the concentrationregulated methanol induction in Komagataella phaffii”
Mol Microbiol, 118:683–697.
2. Inoue, K., Iwase, S., Yurimoto, H., and Sakai, Y.
“Role of the transcription factor Mpp1 in the concentration-regulated methanol induction in Candida
boidinii”
Manuscript in preparation.
3. Ohsawa, S.*, Inoue, K.*, Yurimoto, H., and Sakai Y. (2021)
“Methanol sensor Wsc1 and MAP kinase suppress degradation of methanol-induced peroxisomes in
methylotrophic yeast”
J Cell Sci, 134,
*These authors contributed equally to this work as the first co-author.
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