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6. List of Abbreviations
PER
Period
DBP
Albumin D-site binding protein
CLOCK
Circadian locomotor output cycle kaput
BMAL1
Brain muscle arnt-like 1
CRY
Cryptochrome
CK1
Casein kinase 1
FASP
Familial advanced sleep phase
β-TrCP
Beta-transducin repeat-containing homologue protein
LD
Light dark cycle
DD
Constant darkness
MEF
Mouse embryonic fibroblast
LUC
Luciferase
CHX
Cycloheximide
Dex
Dexamethasone
RING
Really interesting new gene
PAR bZIP
TRAF7
SUMO
Proline- and acidic amino acid-rich basic leucine zipper
5 年以内に雑誌等で刊行予定のため、非公開。
Tumor necrosis factor receptor-associated factor 7
Small ubiquitin like modifier
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7. Acknowledgements
This thesis could not be completed without help of those involved. I would
like to thank everyone who supported me during my research.
Firstly, I am grateful to my supervisor, Dr. Yoshitaka Fukada for training me
to be a scientist. His advices enabled me to make my research more valuable.
In addition, he gave me an opportunity to do research on PER2 mutant mice in
parallel with the study on the DBP proteolysis. Secondly, I would like to express
my gratitude to Dr. Hikari Yoshitane who gave me a million of constructive
comments on my research. I would also like to show my respect and gratitude
to Ms. Rina Nunokawa. She instilled me most of biochemical techniques
required for this thesis. In addition, it would be simply impossible to identify the
regulator unless she performed the DBP-interactome analysis.
I am grateful to co-authors of the paper on PER2 mutant mice, Drs. David
Marc Virshup (Duke-NUS Medical School), Rajesh Narasimamurthy (Duke-NUS
Medical School), Jae Kyoung Kim (Korea Advanced Institute of Science and
Technology).
I would like to thank all the former and current members of Fukada
laboratory, especially Dr. Yohey Ogawa, for beneficial discussion and for
inspiring me.
I am grateful to Drs. Atsu Aiba and Harumi Nakao (The University of
Tokyo) for their help with mouse embryo freezing and the embryo transfer. I
also thank Drs. Jun-ichiro Inoue (The Institute of Medical Science, The
University of Tokyo) and Shigetsugu Hatakeyama (Hokkaido University) for
kindly giving me E2 (DN) expression plasmids and TRIM25 expression plasmid,
respectively.
Finally, I would like to a lot of thanks to my parents to allow and help me to
decide the way.
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