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注釈
本研究は複数の共同研究者とともに行った。本研究において、scRNA-seqデータの取得
は近藤武史博士と共同で行った。また、バルクRNA-seqの実行と解析は近藤武史博士が行
った。加えて、図 24のFISH実験と画像の取得は水野苑子さんが行った。
謝辞
本研究に関して、上村匡教授には私が学部生の頃から非常に熱心な指導をしていただき
ました。ラボセミナーやミーティングでの議論を介して、研究者としての姿勢を学ばせて
いただきました。近藤武史博士には、実験手法、データの取り扱い、文書の作成、研究発
表のしかたなど研究活動を行うにあたって必要な多くの事項を指導していただきました。
また、日々の研究の結果に関して、熱心かつ綿密な議論をしていただき、そのうえで研究
計画について数々の助言をいただきました。碓井理夫博士と坪井有寿博士、春本敏之博士
にはグループミーティングにおいて本研究に関する議論をしていただきました。二股真由
美さん、三木雅代さんには、実験の補助をしていただきました。沖かなえさん、森口良子
さん、今井博子さんには教務関連の補佐をしていただきました。また、他の上村研究室の
メンバーの方々にも多くの有意義な助言をいただきました。以上の上村研究室の皆様の手
助けがなければ、本研究を遂行することはできませんでした。心より感謝申し上げます。
本研究を行うにあたり、上村研究室以外の方々にもお世話になりましたのでお名前を上
げさせていただきます。本研究で用いた空間再構成手法 Perler は広島大学の本田直樹教授
と京都大学医学部附属病院の大河内康史医師との共同研究によるものであり、今回の適用
152
に関しても議論と助言をしていただきました。理化学研究所生命機能科学研究センターの
種子島千春さん、西村理さん、門田満隆さんには scRNA-seq 手法の開発の手助けをしてい
ただきました。井垣研究室の山銅ゆかりさんには、qPCR によるライブラリの定量方法、次
世代シーケンサーの使用法に関して指導をしていただきました。Fluidigm 社の甲斐渉さん
には、C1HT のプライマー設計について助言をいただきました。理化学研究所生命機能科学
研究センターの Yu-Chiun Wang 博士にも本研究に関して助言をいただきました。皆様に厚
くお礼申し上げます。 また、本研究は 2020 年 4 月から 2023 年 3 月までの期間、日本学術振興会の特別研究員
DC1 としての支援を受けて行いました。 最後に、支えてくださった家族や友人にも感謝の言葉を述べさせていただきます。本当
にありがとうございました。 本学位論文は以下の学術論文の内容に基づいて書かれたものです。
Shunta Sakaguchi, Sonoko Mizuno, Yasushi Okochi, Chiharu Tanegashima, Osamu Nishimura,
Tadashi Uemura, Mitsutaka Kadota, Honda Naoki and Takefumi Kondo
Single-cell transcriptome atlas of Drosophila gastrula 2.0
Cell Reports, in press, 2023
坂口
153
峻太 ...
論文の公開元へ
