リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

大学・研究所にある論文を検索できる 「mTORC2はスフィンゴミエリン輸送を促進することにより、低浸透圧ストレスによる細胞死を抑制する」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

コピーが完了しました

URLをコピーしました

論文の公開元へ論文の公開元へ
書き出し

mTORC2はスフィンゴミエリン輸送を促進することにより、低浸透圧ストレスによる細胞死を抑制する

小野, 由美子 ONO, Yumiko オノ, ユミコ 九州大学

2022.04.30

概要

Epithelial cells are constantly exposed to osmotic stress. The influx of water molecules into the cell in a hypo-osmotic environment increases plasma membrane tensions as it rapidly expands. Therefore, the plasma membrane must be supplied with membrane lipids since expansion beyond its elastic limit will cause the cell to rupture. However, the molecular mechanism to maintain a constant plasma membrane tension is not known. In this study, I found that the apical membrane selectively expands when epithelial cells are exposed to hypo-osmotic stress. This requires the activation of mammalian target of rapamycin complex 2 (mTORC2), which enhances the transport of secretory vesicles containing sphingomyelin, the major lipid of the apical membrane. I further show that the mTORC2-Rab35 axis plays an essential role in the defense against hypotonic stress by promoting the degradation of the actin cortex through upregulation of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) metabolism, which facilitates the apical tethering of sphingomyelin-loaded vesicles to relieve plasma membrane tension.

この論文で使われている画像

参考文献

Berchtold, D., M. Piccolis, N. Chiaruttini, I. Riezman, H. Riezman, A. Roux, T.C. Walther, and R. Loewith. 2012. Plasma membrane stress induces relocalization of Slm proteins and activation of TORC2 to promote sphingolipid synthesis. Nat Cell Biol. 14:542-547.

Berchtold, D., and T.C. Walther. 2009. TORC2 plasma membrane localization is essential for cell viability and restricted to a distinct domain. Mol Biol Cell. 20:1565-1575.

Bryant, D.M., A. Datta, A.E. Rodríguez-Fraticelli, J. Peränen, F. Martín-Belmonte, and K.E. Mostov. 2010. A molecular network for de novo generation of the apical surface and lumen. Nat Cell Biol. 12:1035-1045.

Bryant, D.M., J. Roignot, A. Datta, A.W. Overeem, M. Kim, W. Yu, X. Peng, D.J. Eastburn, A.J. Ewald, Z. Werb, and K.E. Mostov. 2014. A molecular switch for the orientation of epithelial cell polarization. Dev Cell. 31:171-187.

Dai, J., and M.P. Sheetz. 1995. Regulation of endocytosis, exocytosis, and shape by membrane tension. Cold Spring Harbor symposia on quantitative biology. 60:567-571.

Dambournet, D., M. Machicoane, L. Chesneau, M. Sachse, M. Rocancourt, A. El Marjou, E. Formstecher, R. Salomon, B. Goud, and A. Echard. 2011. Rab35 GTPase and OCRL phosphatase remodel lipids and F-actin for successful cytokinesis. Nat Cell Biol. 13:981-988.

Deng, Y., F.E. Rivera-Molina, D.K. Toomre, and C.G. Burd. 2016. Sphingomyelin is sorted at the trans Golgi network into a distinct class of secretory vesicle. Proc Natl Acad Sci U S A. 113:6677-6682.

Diz-Muñoz, A., K. Thurley, S. Chintamen, S.J. Altschuler, L.F. Wu, D.A. Fletcher, and O.D. Weiner. 2016. Membrane Tension Acts Through PLD2 and mTORC2 to Limit Actin Network Assembly During Neutrophil Migration. PLoS Biol. 14:e1002474.

Frémont, S., H. Hammich, J. Bai, H. Wioland, K. Klinkert, M. Rocancourt, C. Kikuti, D. Stroebel, G. Romet-Lemonne, O. Pylypenko, A. Houdusse, and A. Echard. 2017. Oxidation of Factin controls the terminal steps of cytokinesis. Nat Commun. 8:14528.

Fukuda, M., E. Kanno, K. Ishibashi, and T. Itoh. 2008. Large scale screening for novel rab effectors reveals unexpected broad Rab binding specificity. Molecular & cellular proteomics : MCP. 7:1031-1042.

Futerman, A.H., B. Stieger, A.L. Hubbard, and R.E. Pagano. 1990. Sphingomyelin synthesis in rat liver occurs predominantly at the cis and medial cisternae of the Golgi apparatus. J Biol Chem. 265:8650-8657.

Gerl, M.J., J.L. Sampaio, S. Urban, L. Kalvodova, J.M. Verbavatz, B. Binnington, D. Lindemann, C.A. Lingwood, A. Shevchenko, C. Schroeder, and K. Simons. 2012. Quantitative analysis of the lipidomes of the influenza virus envelope and MDCK cell apical membrane. J Cell Biol. 196:213-221.

Grahammer, F., S.K. Ramakrishnan, M.M. Rinschen, A.A. Larionov, M. Syed, H. Khatib, M. Roerden, J.O. Sass, M. Helmstaedter, D. Osenberg, L. Kuhne, O. Kretz, N. Wanner, F. Jouret, T. Benzing, F. Artunc, T.B. Huber, and F. Theilig. 2017. mTOR Regulates Endocytosis and Nutrient Transport in Proximal Tubular Cells. Journal of the American Society of Nephrology : JASN. 28:230-241.

Guertin, D.A., D.M. Stevens, C.C. Thoreen, A.A. Burds, N.Y. Kalaany, J. Moffat, M. Brown, K.J. Fitzgerald, and D.M. Sabatini. 2006. Ablation in mice of the mTORC components raptor, rictor, or mLST8 reveals that mTORC2 is required for signaling to Akt-FOXO and PKCalpha, but not S6K1. Dev Cell. 11:859-871

Ikenouchi, J., M. Hirata, S. Yonemura, and M. Umeda. 2013. Sphingomyelin clustering is essential for the formation of microvilli. J Cell Sci. 126:3585-3592.

Ikenouchi, J., M. Suzuki, K. Umeda, K. Ikeda, R. Taguchi, T. Kobayashi, S.B. Sato, T. Kobayashi, D.B. Stolz, and M. Umeda. 2012. Lipid polarity is maintained in absence of tight junctions. J Biol Chem. 287:9525-9533.

Janmey, P.A., R. Bucki, and R. Radhakrishnan. 2018. Regulation of actin assembly by PI(4,5)P2 and other inositol phospholipids: An update on possible mechanisms. Biochem Biophys Res Commun. 506:307-314.

Kiyokawa, E., A. Makino, K. Ishii, N. Otsuka, A. Yamaji-Hasegawa, and T. Kobayashi. 2004. Recognition of sphingomyelin by lysenin and lysenin-related proteins. Biochemistry. 43:9766-9773.

Klinkert, K., M. Rocancourt, A. Houdusse, and A. Echard. 2016. Rab35 GTPase couples cell division with initiation of epithelial apico-basal polarity and lumen opening. Nat Commun. 7:11166.

Kozera, L., E. White, and S. Calaghan. 2009. Caveolae act as membrane reserves which limit mechanosensitive I(Cl,swell) channel activation during swelling in the rat ventricular myocyte. PLoS One. 4:e8312.

Le Roux, A.L., X. Quiroga, N. Walani, M. Arroyo, and P. Roca-Cusachs. 2019. The plasma membrane as a mechanochemical transducer. Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 374:20180221.

Liu, G.Y., and D.M. Sabatini. 2020. mTOR at the nexus of nutrition, growth, ageing and disease. Nat Rev Mol Cell Biol. 21:183-203.

Martin-Belmonte, F., A. Gassama, A. Datta, W. Yu, U. Rescher, V. Gerke, and K. Mostov. 2007. PTEN-mediated apical segregation of phosphoinositides controls epithelial morphogenesis through Cdc42. Cell. 128:383-397.

Meder, D., A. Shevchenko, K. Simons, and J. Füllekrug. 2005. Gp135/podocalyxin and NHERF- 2 participate in the formation of a preapical domain during polarization of MDCK cells. J Cell Biol. 168:303-313.

Mrozowska, P.S., and M. Fukuda. 2016. Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures. J Cell Biol. 213:355-369.

Muir, A., S. Ramachandran, F.M. Roelants, G. Timmons, and J. Thorner. 2014. TORC2-dependent protein kinase Ypk1 phosphorylates ceramide synthase to stimulate synthesis of complex sphingolipids. Elife. 3.

Niles, B.J., H. Mogri, A. Hill, A. Vlahakis, and T. Powers. 2012. Plasma membrane recruitment and activation of the AGC kinase Ypk1 is mediated by target of rapamycin complex 2 (TORC2) and its effector proteins Slm1 and Slm2. Proc Natl Acad Sci U S A. 109:1536-1541.

Pietuch, A., B.R. Brückner, and A. Janshoff. 2013. Membrane tension homeostasis of epithelial cells through surface area regulation in response to osmotic stress. Biochim Biophys Acta. 1833:712-722.

Pontes, B., P. Monzo, and N.C. Gauthier. 2017. Membrane tension: A challenging but universal physical parameter in cell biology. Semin Cell Dev Biol. 71:30-41.

Rai, A., A. Oprisko, J. Campos, Y. Fu, T. Friese, A. Itzen, R.S. Goody, E.M. Gazdag, and M.P. Müller. 2016. bMERB domains are bivalent Rab8 family effectors evolved by gene duplication. Elife. 5.

Riggi, M., K. Niewola-Staszkowska, N. Chiaruttini, A. Colom, B. Kusmider, V. Mercier, S. Soleimanpour, M. Stahl, S. Matile, A. Roux, and R. Loewith. 2018. Decrease in plasma membrane tension triggers PtdIns(4,5)P(2) phase separation to inactivate TORC2. Nat Cell Biol. 20:1043-1051.

Roelants, F.M., D.K. Breslow, A. Muir, J.S. Weissman, and J. Thorner. 2011. Protein kinase Ypk1 phosphorylates regulatory proteins Orm1 and Orm2 to control sphingolipid homeostasis in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 108:19222-19227.

Shiomi, R., K. Shigetomi, T. Inai, M. Sakai, and J. Ikenouchi. 2015. CaMKII regulates the strength of the epithelial barrier. Sci Rep. 5:13262.

Sinha, B., D. Köster, R. Ruez, P. Gonnord, M. Bastiani, D. Abankwa, R.V. Stan, G. Butler-Browne, B. Vedie, L. Johannes, N. Morone, R.G. Parton, G. Raposo, P. Sens, C. Lamaze, and P. Nassoy. 2011. Cells respond to mechanical stress by rapid disassembly of caveolae. Cell. 144:402-413.

Strilić, B., T. Kucera, J. Eglinger, M.R. Hughes, K.M. McNagny, S. Tsukita, E. Dejana, N. Ferrara, and E. Lammert. 2009. The molecular basis of vascular lumen formation in the developing mouse aorta. Dev Cell. 17:505-515.

Yamaji, A., Y. Sekizawa, K. Emoto, H. Sakuraba, K. Inoue, H. Kobayashi, and M. Umeda. 1998. Lysenin, a novel sphingomyelin-specific binding protein. J Biol Chem. 273:5300-5306.

Yang, J., X. Zhao, A. Patel, R. Potru, S. Azizi-Ghannad, M. Dolinger, J. Cao, C. Bartholomew, J. Mazurkiewicz, D. Conti, D. Jones, Y. Huang, and X.C. Zhu. 2015. Rapamycin Inhibition of mTOR Reduces Levels of the Na+/H+ Exchanger 3 in Intestines of Mice and Humans, Leading to Diarrhea. Gastroenterology. 149:151-162.

Zhang, Q., A. Schepis, H. Huang, J. Yang, W. Ma, J. Torra, S.Q. Zhang, L. Yang, H. Wu, S. Nonell, Z. Dong, T.B. Kornberg, S.R. Coughlin, and X. Shu. 2019. Designing a Green Fluorogenic Protease Reporter by Flipping a Beta Strand of GFP for Imaging Apoptosis in Animals. Journal of the American Chemical Society. 141:4526-4530.

参考文献をもっと見る

全国の大学の
卒論・修論・学位論文

一発検索!

この論文の関連論文を見る