Adachi, K., Nikaido, I., Ohta, H., Ohtsuka, S., Ura, H., Kadota, M., Wakayama, T.,
Ueda, H.R., Niwa, H., 2013. Context-dependent wiring of Sox2 regulatory networks
for self-renewal of embryonic and trophoblast stem cells. Mol. Cell 52, 380–392.
Ando, Y., Okeyo, K.O., Adachi, T., 2019. Modulation of adhesion microenvironment
using mesh substrates triggers selforganization and primordial germ cell-like
differentiation in mouse ES cells. APL Bioeng. 3, 016102.
Belly, H.D., Stubb, A., Yanagida, A., Labouesse, C., Jones, P.H., Paluch, E.K., Chalut, K.J.,
2021. Membrane tension gates ERK-mediated regulation of pluripotent cell fate. Cell
Stem Cell 28, 273–284.
Blanchard, G.B., Kabla, A.J., Schultz, N.L., Butler, L.C., Sanson, B., Gorfinkiel, N.,
Mahadevan, L., Adams, R.J., 2009. Tissue tectonics: morphogenetic strain rates, cell
shape change and intercalation. Nat. Methods 6, 458–464.
David, B.G., Fujita, H., Yasuda, K., Okamoto, K., Panina, Y., Ichinose, J., Sato, O.,
Horie, M., Ichimura, T., Okada, Y., Watanabe, T.M., 2019. Linking substrate and
nucleus via actin cytoskeleton in pluripotency maintenance of mouse embryonic
stem cells. Stem Cell Res. 41, 101614.
Desmaison, A., Guillaume, L., Triclin, S., Weiss, P., Ducommun, B., Lobjois, V., 2018.
Impact of physical confinement on nuclei geometry and cell division dynamics in 3D
spheroids. Sci. Rep. 8, 8785.
Du, J., Fan, Y., Guo, Z., Wang, Y., Zheng, X., Huang, C., Liang, B., Gao, L., Cao, Y.,
Chen, Y., Zhang, X., Li, L., Xu, L., Wu, C., Weitz, D.A., Feng, X., 2019. Compression
generated by a 3D supracellular actomyosin cortex promotes embryonic stem cell
colony growth and expression of Nanog and Oct4. Cell Syst. 9, 214–220.
CRediT authorship contribution statement
Yuta Ando: Investigation, Methodology, Writing - original draft,
Visualization. Kennedy Omondi Okeyo: Conceptualization, Method
ology, Writing - review & editing, Resources, Funding acquisition.
Junko Sunaga: Investigation, Resources. Taiji Adachi: Writing - review
& editing, Resources, Supervision, Funding acquisition.
10
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Kyoto University Research Information Repository
https://repository.kulib.kyoto-u.ac.jp
Y. Ando et al.
Stem Cell Research 53 (2021) 102352
Mendoza, M.C., Vilela, M., Juarez, J.E., Blenis, J., Danuser, G., 2015. ERK reinforces
actin polymerization to power persistent edge protrusion during motility. Sci. Signal.
8, ra47.
Miyoshi, H., Adachi, T., 2014. Topography design concept of a tissue engineering
scaffold for controlling cell function and fate through actin cytoskeletal modulation.
Tissue Eng. Part B Rev. 20, 609–627.
Okeyo, K.O., Kurosawa, O., Oana, H., Kotera, H., Washizu, M., 2016. Minimization of
cell-substrate interaction using suspended microstructured meshes initiates cell sheet
formation by self-assembly organization. Biomed. Phys. Eng. Express 2, 065019.
Okeyo, K.O., Kurosawa, O., Yamazaki, S., Oana, H., Kotera, H., Nakauchi, H.,
Washizu, M., 2015. Cell adhesion minimization by a novel mesh culture method
mechanically directs trophoblast differentiation and self-assembly organization of
human pluripotent stem cells. Tissue Eng. Part C Methods 21, 1105–1115.
Okeyo, K.O., Tanabe, M., Kurosawa, O., Oana, H., Washizu, M., 2018. Self-organization
of human iPS cells into trophectoderm mimicking cysts induced by adhesion
restriction using microstructured mesh scaffolds. Dev. Growth Differ. 1, 1–13.
Ollion, J., Cochennec, J., Loll, F., Escud´e, C., Boudier, T., 2013. TANGO: a generic tool
for high-throughput 3D image analysis for studying nuclear organization.
Bioinformatics 29, 1840–1841.
Riedl, J., Crevenna, A.H., Kessenbrock, K., Yu, J.H., Neukirchen, D., Bista, M., Bradke, F.,
Jenne, D., Holak, T.A., Werb, Z., Sixt, M., Wedlich-Soldner, R., 2008. Lifeact: a
versatile marker to visualize F-actin. Nat. Methods 5, 605–607.
Rosowski, K., Mertz, A.F., Norcross, S., Dufresne, E.R., Horsley, V., 2015. Edges of human
embryonic stem cell colonies display distinct mechanical properties and
differentiation potential. Sci. Rep. 5, 14218.
Sagy, N., Slovin, S., Allalouf, M., Pour, M., Savyon, G., Boxman, J., Nachman, I., 2019.
Prediction and control of symmetry breaking in embryoid bodies by environment
and signal integration. Development 146, dev181917.
Shao, Y., Taniguchi, K., Gurdziel, K., Townshend, R.F., Xue, X., Yong, K.M.A., Sang, J.,
Spence, J.R., Gumucio, D.L., Fu, J., 2017. Self-organized amniogenesis by human
pluripotent stem cells in a biomimetic implantation-like niche. Nat. Mater. 16,
419–425.
Shahbazi, M.N., Siggia, E.D., Zernicka-Goetz, M., 2019. Self-organization of stem cells
into embryos: A window on early mammalian development. Science 364, 948–951.
Smith, K.N., Singh, A.M., Dalton, S., 2010. Myc represses primitive endoderm
differentiation in pluripotent stem cells. Cell Stem Cell 7, 343–354.
Takata, N., Sakakura, E., Eiraku, M., Kasukawa, T., Sasai, Y., 2017. Self-patterning of
rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt
antagonism. Nat. Commun. 8, 1339.
Takebe, T., Wells, J.M., 2019. Organoids by design. Science 364, 956–959.
Toyooka, Y., Shimosato, D., Murakami, K., Takahashi, K., Niwa, H., 2008. Identification
and characterization of subpopulations in undifferentiated ES cell culture.
Development 135, 909–918.
Verstreken, C.M., Labouesse, C., Agley, C.C., Chalut, C.C., 2019. Embryonic stem cells
become mechanoresponsive upon exit from ground state of pluripotency. Open Biol.
9, 180203.
Warmflash, A., Sorre, B., Etoc, F., Siggia, E.D., Brivanlou, A.H., 2014. A method to
recapitulate early embryonic spatial patterning in human embryonic stem cells. Nat.
Methods 11, 847–854.
Xie, A.W., Binder, B.Y.K., Khalil, A.S., Schmitt, S.K., Johnson, H.J., Zacharias, N.A.,
Murphy, W.L., 2017. Controlled self-assembly of stem cell aggregates instructs
pluripotency and lineage bias. Sci. Rep. 7, 14070.
Yang, S.-H., Kalkan, T., Morrisroe, C., Smith, A., Sharrocks, A.D., 2012. A genome-wide
RNAi screen reveals MAP kinase phosphatases as key ERK pathway regulators during
embryonic stem cell differentiation. PLoS Genet. 8, e1003112.
Eiraku, M., Takata, N., Ishibashi, H., Kawada, M., Sakakura, E., Okuda, S., Sekiguchi, K.,
Adachi, T., Sasai, Y., 2011. Self-organizing optic-cupmorphogenesis in threedimensional culture. Nature 472, 51–56.
Guilak, F., Cohen, D.M., Estes, B.T., Gimble, J.M., Liedtke, W., Chen, C.S., 2009. Control
of stem cell fate by physical interactions with the extracellular matrix. Cell Stem Cell
5, 17–26.
Harrison, S.E., Sozen, B., Christodoulou, N., Kyprianou, C., Zernicka-Goetz, M., 2017.
Assembly of embryonic and extraembryonic stem cells to mimic embryogenesis in
vitro. Science 356, eaal1810.
Hayashi, K., Ohta, H., Kurimoto, K., Aramaki, S., Saitou, M., 2011. Reconstitution of the
mouse germ cell specification pathway in culture by pluripotent stem cells. Cell 146,
519–532.
Heer, N.C., Martin, A.C., 2017. Tension, contraction and tissue morphogenesis.
Development 144, 4249–4260.
Hiramatsu, R., Matsuoka, T., Kimura-Yoshida, C., Han, S., Mochida, K., Adachi, T.,
Takayama, S., Matsuo, I., 2013. External mechanical cues trigger the establishment
of the anterior-posterior axis in early mouse embryos. Dev. Cell 27, 131–144.
Hirata, H., Sokabe, M., 2015. A novel role of actomyosin in ERK singaling. Commun.
Integr. Biol. 8, e1017176.
Hishida, T., Nozaki, Y., Nakachi, Y., Mizuno, Y., Okazaki, Y., Ema, M., Takahashi, S.,
Nishimoto, M., Okuda, A., 2011. Indefinite self-renewal of ESCs through Myc/Max
transcriptional complex-independent mechanisms. Cell Stem Cell 9, 37–49.
Hooper, M., Hardy, K., Handyside, A., Hunter, S., Monk, M., 1987. HPRT-deficient
(Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells.
Nature 326, 292–295.
Karzbrun, E., Kshirsagar, A., Cohen, S.R., Hanna, J.H., Reiner, O., 2018. Human brain
organoids on a chip reveal the physics of folding. Nat. Phys. 14, 515–522.
Kimura, T., Kaga, Y., Ohta, H., Odamoto, M., Sekita, Y., Li, K., Yamano, N., Fujikawa, K.,
Isotani, A., Sasaki, N., Toyoda, M., Hayashi, K., Okabe, M., Shinohara, T., Saitou, M.,
Nakano, T., 2014. Induction of primordial germ cell-like cells from mouse embryonic
stem cells by ERK signal inhibition. Stem Cells 32, 2668–2678.
Kshitiz, Park, J., Kim, P., Helen, W., Engler, A.J., Levchenko, A., Kim, D-H., 2012.
Control of stem cell fate and function by engineering physical microenvironment.
Integr. Biol. 4, 1008–1018.
Kumar, A., Placone, J.K., Engler, A.J., 2017. Understanding the extracellular forces that
determine cell fate and maintenance. Development 144, 4261–4270.
Latorre, E., Kale, S., Casares, L., G´
omez-Gonz´
alez, M., Uroz, M., Valon, L., Nair, R.V.,
Garreta, E., Montserrat, N., Del Campo, A., Ladoux, B., Arroyo, M., Trepat, X., 2018.
Active superelasticity in three-dimensional epithelia of controlled shape. Nature 563,
203–208.
Li, J., Minami, I., Shiozaki, M., Yu, L., Yajima, S., Miyagawa, S., Shiba, Y., Morone, N.,
Fukushima, S., Yoshida, M., Li, S., Qiao, L., Li, X., Wang, L., Kotera, H., Nakatsuji, N.,
Sawa, Y., Chen, Y., Liu, L., 2017. Human pluripotent stem cell-derived cardiac tissuelike constructs for repairing the infarcted myocardium. Stem Cell Rep. 9,
1546–1559.
Li, Z., Kurosawa, O., Iwata, H., 2019. Establishment of human trophoblast stem cells
from human induced pluripotent stem cell-derived cystic cells under micromesh
culture. Stem Cell Res. Therapy 10, 245.
Maeda, I., Okamura, D., Tokitake, Y., Ikeda, M., Kawaguchi, H., Mise, N., Abe, K.,
Noce, T., Okuda, A., Matsui, Y., 2013. Max is a repressor of germ cell-related gene
expression in mouse embryonic stem cells. Nat. Commun. 4, 1754.
Matsushiro, Y., Kato-Negeshi, M., Onoe, H., 2018. Differentiation of 3D-shape-controlled
mouse neural stem cell to neural tissues in closed agarose microchambers. Biotech.
Bioeng. 115, 1614–1623.
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