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High-performance pipeline for MutMap and QTL-seq

Sugihara, Yu Young, Lester Yaegashi, Hiroki Natsume, Satoshi Shea, Daniel J. Takagi, Hiroki Booker, Helen Innan, Hideki Terauchi, Ryohei Abe, Akira 京都大学 DOI:10.7717/peerj.13170

2022

概要

[Summary] Bulked segregant analysis implemented in MutMap and QTL-seq is a powerful and efficient method to identify loci contributing to important phenotypic traits. However, the previous pipelines were not user-friendly to install and run. Here, we describe new pipelines for MutMap and QTL-seq. These updated pipelines are approximately 5–8 times faster than the previous pipeline, are easier for novice users to use, and can be easily installed through bioconda with all dependencies. [Availability] The new pipelines of MutMap and QTL-seq are written in Python and can be installed via bioconda. The source code and manuals are available online (MutMap: https://github.com/YuSugihara/MutMap, QTL-seq: https://github.com/YuSugihara/QTL-seq).

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参考文献

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