Establishment of induced pluripotent stem cells from schizophrenia discordant fraternal twins

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4.6. Flow cytometry analysis

iPSCs were treated with Accumax (Innovative Cell Technologies, San

Diego, CA, US) and dissociated into single cells. Then, the cells were

incubated at 1.0 × 106 cells/ml concentration in PBS containing 2% FBS

and 20 µl SSEA-4 APC conjugated monoclonal antibody (BD Biosciences,

Franklin Lakes, NJ, US) for 30 min at 4 ◦ C. The cells were washed twice

with PBS containing 2% FBS and analyzed by FACSAria (BD

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