リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

大学・研究所にある論文を検索できる 「Aqueous humor SPARC concentration in canine glaucomas」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。
リケラボ

コピーが完了しました

URLをコピーしました

論文の公開元へ論文の公開元へ
書き出し

Aqueous humor SPARC concentration in canine glaucomas

Kubo, Akira Miyasho, Taku Nakamura, Kozo Ito, Yosuke Endo, Yusuke Masuko, Arisa Hayashi, Miri Maehara, Seiya Nakade, Tetsuya 北海道大学

2021.11

概要

This study was conducted to identify new objective parameters to diagnose and etiologically differentiate canine glaucomas. In the first phase, proteomic analysis was performed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption/ionization time- of-flight mass spectrometry (MS) on the aqueous humor of each single eye of five healthy dogs, three dogs with primary glaucoma, and three dogs with secondary glaucoma. Secreted protein acidic and rich in cysteine (SPARC) was identified as a protein that was expressed in all healthy dogs but not in any of glaucomatous dogs. In the second part of the study, we examined SPARC concentrations in the aqueous humor of each single eye of 13 healthy dogs, 14 dogs with primary glaucoma and 13 dogs with secondary glaucoma by enzyme-linked immunosorbent assay. The SPARC concentration was numerically lower with primary glaucoma (0.486 ± 0.047 ng/ml, mean ± standard error) and numerically higher with secondary glaucoma (0.738 ± 0.089 ng/ml) compared to the control eyes (0.637 ± 0.081 ng/ml). Receiver operating characteristic curve analysis confirmed the ability of the aqueous humor SPARC concentration to differentiate between primary and secondary glaucomas (AUC = 0.72). Thus, 2D-PAGE and MS might be valuable tools for screening of the aqueous humor proteins in canine glaucoma, and the SPARC concentration has a clinical potential in the etiological classification of canine glaucomas.

論文の公開元へ

この論文で使われている画像

関連論文

関連論文をもっと見る

参考文献

1) Andriani F, Landoni E, Mensah M, Facchinetti F, Miceli R, Tagliabue E, Giussani M, Callari M, Cecco LD, Colombo MP, Roz L, Pastrino U, Sozzi G. Diagnostic role of circulating extracellular matrix-related proteins in non- small cell lung cancer. BMC Cancer 18, 899, 2018.

2) Bornstein P, Sage EH. Matricellular proteins: extracellular modulators of cell function. Curr Opin Cell Biol 14(5), 608-616, 2002.

3) Brekken RA, Sage EH. SPARC, a matricellular protein: at the crossroads of cell- matrix communication. Matrix Biol 19(8), 816-827, 2001.

4) Chiang SY, Tsai ML, Wang CY, Chen A, Chou YC, Hsia CW, Wu YF, Chen HM, Huang TH, Chen PH, Liu HT, Shui HA. Proteomic analysis and identification of aqueous humor proteins with a pathophysiological role in diabetic retinopathy. J Proteomics 75(10), 2950-2959, 2012.

5) Chua J, Seet LF, Jiang YZ, Su R, Htoon HM, Charlton A, Aung T, Wong TT. Increased SPARC expression in primary angle closure glaucoma iris. Mol Vis 14, 1886-1892, 2008.

6) Critselis E, Rava M, Marquez M, Lygirou V, Ch atz i c h ar alambo u s D , Li api P, Lichtinghagen R, Brand K, Cecchini L, Vlahou A, Malats N, Zoidakis J. Diagnostic and prognostic performance of secreted protein acidic and rich in cysteine (SPARC) assay for detecting primary and recurrent urinary bladder cancer. Proteomics Clin Appl 13(2), e1800148, 2019.

7) Duan X, Xue P, Wang N, Dong Z, Lu Q, Yang F. Proteomic analysis of aqueous humor from patients with primary open angle glaucoma. Mol Vision 16, 2839-2846, 2010.

8) Featherstone HJ, Heinrich CL. Ophthalmic examination and diagnostics. In: Veterinary Ophthalmology, 5th ed. Gelatt KN, Gilger BC, Kern TJ eds. Blackwell Publishing, Ames, pp. 533-613, 2013.

9) Grus FH, Joachim SC, Sandmann S, Thiel S, Bruns K, Lackner KJ, Pfeiffer N. Transthyretin and complex protein pattern in aqueous humor of patients with primary open-angle glaucoma. Mol Vision 14, 1437-1445, 2008.

10) Gum GG, MacKay EO. Physiology of the eye. In: Veterinary Ophthalmology, 5th ed. Gelatt KN, Gilger BC, Kern TJ eds. Blackwell Publishing, Ames, pp. 171-207, 2013.

11) Haddadin RI, Oh DJ, Kang MH, Filippopoulos T, Gupta M, Hart L, Sage EH, Rhee DJ. SPARC-null mice exhibit lower intraocular pressures. Invest Ophthalmol Vis Sci 50(8), 3771-3777, 2009.

12) Itoh Y, Umeda Y, Wakaiki S, Saito A. Study of vision maintenance and duration for consultation with veterinary clinic in canine glaucomatous case. Journal of the Japan Veterinary Medical Association 63, 281-285, 2010.

13) Kubo A, Ito Y, Masuko A, Maehara S, Miyasho T, Nakade T. Vision outcome with antiglaucoma therapy and prognostic factors in canine glaucoma: A 6-year retrospective study in Japan. Jpn J Vet Res 67(1), 93-102, 2019.

14) Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685, 1970.

15) Miller PE. The glaucomas. In: Slatter’s Fundamentals of Veterinary Ophthalmology, 5th ed. Maggs DJ, Miller PE, Ofri R. Elsevier Saunders, St. Louis. pp. 257-271, 2013.

16) Miller PE, Schmidt GM, Vainisi SJ, Swanson JF, Herrmann MK. The efficacy of topical prophylactic antiglaucoma therapy in primary closed angle glaucoma in dogs: A multicenter clinical trial. J Am Anim Hosp Assoc 36(5), 431-438, 2000.

17) Oh DJ, Kang MH, Ooi YH, Choi KR, Sage EH, Rhee DJ. Overexpression of SPARC in human trabecular meshwork increases intraocular pressure and alters extracellular matrix. Invest Ophthalmol Vis Sci 54, 3309-3319, 2013.

18) Papapanagiotou A, Sgourakis G, Karkoulias K, Raptis D, Parkin E, Brotzakis P, Panchal S, Papavassiliou AG. Osteonectin as a screening marker for pancreatic cancer: A prospective study. J Int Med Res 46(7), 2769-2779, 2018.

19) Plummer CE, Regnier A, Gelatt KN. The canine glaucoma. In: Veterinary Ophthalmology, 5th ed. Gelatt KN, Gilger BC, Kern TJ eds. Blackwell Publishing, Ames, pp. 1050-1145, 2013.

20) Stastna M, Behrens A, McDonnell PJ, Van Eyk JE. Analysis of protein composition of rabbit aqueous humor following two different cataract surgery incision procedures using 2-DE and LC-MS/MS. Proteome Sci 9(1), 8, 2011.

21) Stastna M, Behrens A, Noguera G, Herretes S, McDonnell P, Van Eyk, JE. Proteomics of the aqueous humor in healthy New Zealand rabbits. Proteomics 7(23), 4358-4375, 2007.

参考文献をもっと見る

全国の大学の
卒論・修論・学位論文
を
一発検索！

この論文の関連論文を見る