1. Walsh, G. Biopharmaceutical benchmarks 2018. Nat. Biotechnol. 36, 1136–1145 (2018).
2. Wurm, F. M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 22, 1393–1398 (2004).
3. Post, M. J. An alternative animal protein source: Cultured beef: Cultured beef. Ann. N. Y. Acad. Sci. 1328, 29–33 (2014).
4. Tuomisto, H. L. & de Mattos, M. J. T. Environmental impacts of cultured meat production. Environ. Sci. Technol. 45, 6117–6123
(2011).
5. Hubalek, S., Post, M. J. & Moutsatsou, P. Towards resource-efficient and cost-efficient cultured meat. Curr. Opin. Food Sci. 47,
100885 (2022).
6. Okamoto, Y., Haraguchi, Y., Sawamura, N., Asahi, T. & Shimizu, T. Mammalian cell cultivation using nutrients extracted from
microalgae. Biotechnol. Prog. https://doi.org/10.1002/btpr.2941 (2020).
7. Okamoto, Y. et al. Proliferation and differentiation of primary bovine myoblasts using Chlorella vulgaris extract for sustainable
production of cultured meat. Biotechnol. Prog. https://doi.org/10.1002/btpr.3239 (2022).
8. Haraguchi, Y., Okamoto, Y. & Shimizu, T. A circular cell culture system using microalgae and mammalian myoblasts for the
production of sustainable cultured meat. Arch. Microbiol. https://doi.org/10.1007/s00203-022-03234-9 (2022).
9. Haraguchi, Y. & Shimizu, T. Microalgal culture in animal cell waste medium for sustainable ‘cultured food’ production. Arch.
Microbiol. 203, 5525–5532 (2021).
10. Schneider, M. The importance of ammonia in mammalian cell culture. J. Biotechnol. 46, 161–185 (1996).
11. Hidese, R., Matsuda, M., Osanai, T., Hasunuma, T. & Kondo, A. Malic enzyme facilitates d-lactate production through increased
pyruvate supply during anoxic dark fermentation in Synechocystis sp. PCC 6803. ACS Synth. Biol. 9, 260–268 (2020).
12. Selão, T. T., Jebarani, J., Ismail, N. A., Norling, B. & Nixon, P. J. Enhanced production of D-lactate in Cyanobacteria by re-routing
photosynthetic cyclic and pseudo-cyclic electron flow. Front. Plant Sci. https://doi.org/10.3389/fpls.2019.01700 (2020).
13. Joseph, A. et al. Utilization of lactic acid bacterial genes in Synechocystis sp. PCC 6803 in the production of lactic acid. Biosci.
Biotechnol. Biochem. 77, 966–970 (2013).
14. Aguilera, L. et al. Dual role of LldR in regulation of the lldPRD operon, involved in l -lactate metabolism in Escherichia coli. J.
Bacteriol. 190, 2997–3005 (2008).
15. Gao, C. et al. Lactate utilization is regulated by the FadR-type regulator LldR in Pseudomonas aeruginosa. J. Bacteriol. 194, 2687–
2692 (2012).
16. Stansen, C. et al. Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperaturetriggered glutamate production. Appl. Environ. Microbiol. 71, 5920–5928 (2005).
17. Kanehisa, M. & Goto, S. KEGG: Kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 28, 27–30 (2000).
18. Jiang, T., Gao, C., Ma, C. & Xu, P. Microbial lactate utilization: Enzymes, pathogenesis, and regulation. Trends Microbiol. 22,
589–599 (2014).
19. Wang, L., Cai, Y., Zhu, L., Guo, H. & Yu, B. Major role of NAD-dependent lactate dehydrogenases in the production of l-lactic acid
with high optical purity by the thermophile Bacillus coagulans. Appl. Environ. Microbiol. 80, 7134–7141 (2014).
20. Gobler, C. J. et al. Niche of harmful alga Aureococcus anophagefferens revealed through ecogenomics. Proc. Natl. Acad. Sci. U. S.
A. 108, 4352–4357 (2011).
21. Bench, S. R., Ilikchyan, I. N., Tripp, H. J. & Zehr, J. P. Two strains of Crocosphaera watsonii with highly conserved genomes are
distinguished by strain-specific features. Front. Microbiol. https://doi.org/10.3389/fmicb.2011.00261 (2011).
22. Boatman, T. G., Lawson, T. & Geider, R. J. A key marine diazotroph in a changing ocean: The interacting effects of temperature,
CO2 and light on the growth of Trichodesmium erythraeum IMS101. PLoS One 12, e0168796 (2017).
23. Sihvonen, L. M. et al. Strains of the cyanobacterial genera Calothrix and Rivularia isolated from the Baltic Sea display cryptic
diversity and are distantly related to Gloeotrichia and Tolypothrix: Baltic Sea Calothrix are genetically diverse. FEMS Microbiol.
Ecol. 61, 74–84 (2007).
Scientific Reports |
(2023) 13:7249 |
https://doi.org/10.1038/s41598-023-34289-3
Vol.:(0123456789)
www.nature.com/scientificreports/
24. Hasunuma, T. et al. Single-stage astaxanthin production enhances the nonmevalonate pathway and photosynthetic central metabolism in Synechococcus sp. PCC 7002. ACS Synth. Biol. 8, 2701–2709 (2019).
25. Mihara, S., Sugiura, K., Yoshida, K. & Hisabori, T. Thioredoxin targets are regulated in heterocysts of cyanobacterium Anabaena
sp. PCC 7120 in a light-independent manner. J. Exp. Bot. 71, 2018–2027 (2020).
26. Hasunuma, T. et al. Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion.
J. Exp. Bot. 64, 2943–2954 (2013).
27. Kato, Y. et al. Enhancing carbohydrate repartitioning into lipid and carotenoid by disruption of microalgae starch debranching
enzyme. Commun. Biol. 4, 450 (2021).
28. Kanamoto, A., Kato, Y., Yoshida, E., Hasunuma, T. & Kondo, A. Development of a method for fucoxanthin production using the
haptophyte marine microalga pavlova sp. OPMS 30543. Mar. Biotechnol. 23, 331–341 (2021).
29. Li, C. et al. Enhancing the light-driven production of d-lactate by engineering cyanobacterium using a combinational strategy.
Sci. Rep. 5, 9777 (2015).
30. Núñez, M. F. et al. Transport of -lactate, -lactate, and glycolate by the LldP and GlcA membrane carriers of Escherichia coli. Biochem.
Biophys. Res. Commun. 290, 824–829 (2002).
31. Benson, P. J. et al. Factors altering pyruvate excretion in a glycogen storage mutant of the Cyanobacterium, Synechococcus PCC7942.
Front. Microbiol. https://doi.org/10.3389/fmicb.2016.00475 (2016).
32. Gründel, M., Scheunemann, R., Lockau, W. & Zilliges, Y. Impaired glycogen synthesis causes metabolic overflow reactions and
affects stress responses in the cyanobacterium Synechocystis sp. PCC 6803. Microbiology 158, 3032–3043 (2012).
33. Jackson, S. A., Eaton-Rye, J. J., Bryant, D. A., Posewitz, M. C. & Davies, F. K. Dynamics of photosynthesis in a glycogen-deficient
glgC mutant of Synechococcus sp. strain PCC 7002. Appl. Environ. Microbiol. 81, 6210–6222 (2015).
34. Yao, T. & Asayama, Y. Animal-cell culture media: History, characteristics, and current issues. Reprod. Med. Biol. 16, 99–117 (2017).
35. Shimakawa, G., Kohara, A. & Miyake, C. Characterization of light-enhanced respiration in Cyanobacteria. Int. J. Mol. Sci. 22, 342
(2020).
Acknowledgements
We thank Ms. Aya Narita and Ms. Mami Matsuda for their technical assistance. This study was financially supported by the Cabinet Office, Government of Japan, Moonshot Research and Development Program.
Author contributions
Y.K.: Conceptualization, investigation, methodology, writing–original draft. K.I.: Investigation. Y.H.: Conceptualization, writing–review and editing. T.S.: Conceptualization, project administration. A.K.: Supervision. T.H.:
Project administration, writing–review and editing.
Competing interests The authors declare no competing interests.
Additional information
Supplementary Information The online version contains supplementary material available at https://doi.org/
10.1038/s41598-023-34289-3.
Correspondence and requests for materials should be addressed to T.H.
Reprints and permissions information is available at www.nature.com/reprints.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and
institutional affiliations.
Open Access This article is licensed under a Creative Commons Attribution 4.0 International
License, which permits use, sharing, adaptation, distribution and reproduction in any medium or
format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the
Creative Commons licence, and indicate if changes were made. The images or other third party material in this
article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the
material. If material is not included in the article’s Creative Commons licence and your intended use is not
permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from
the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
© The Author(s) 2023
Scientific Reports |
Vol:.(1234567890)
(2023) 13:7249 |
https://doi.org/10.1038/s41598-023-34289-3
...
論文の公開元へ
