リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

大学・研究所にある論文を検索できる 「Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。
リケラボ

コピーが完了しました

URLをコピーしました

論文の公開元へ論文の公開元へ
書き出し

Microbiome analysis of contact lens care solutions and tear fluids of contact lens wearers: Possible involvement of streptococcal antigens in allergic symptoms related to contact lens wear

堀田 芙美香 近畿大学

2022.06.15

概要

【目的】
分子生物学的手法を用いてアレルギー症状（AS）を有するコンタクトレンズ（CL）装用者の CL 保存液と涙液を分析することにより、CL 装用に伴う AS の病因を解明すること。

【方法】
AS を有する CL 装用者 9 例（AS 群）と無症状の CL 装用者 6 例（健常群）を対象とし、それぞれから CL 保存ケースと涙液を回収した。CL 保存ケース内の保存液と涙液に含まれる細菌群集を培養法と 16S rDNA シーケンシングによって分析した。涙液中のヒスタミンレベルを高速液体クロマトグラフィーで測定した。AS 群の涙液中 IgE が結合する細菌を同定するために、ウエスタンブロット法を行った。

【結果】
AS 群と健常群の間で、CL 保存液の培養陽性率やコロニー形成単位に有意差はなかった。1 例を除いて、涙液から細菌は分離されなかった。CL 保存液のメタ 16S 解析の結果、CL 保存液から多様な細菌が検出され、AS 群 9 例は菌叢パターンにより 3 つグループに分けられた（CL-I,II,III group）。このうち、CL-I group（4 例）は健常群とは明らかに異なる菌叢パターンを示した。ヒスタミンは AS 群の 2 例から検出され、その 2 例は CL-I group に属していた。CL-I group では、CL 保存液の菌叢に占めるグラム陽性菌の相対的存在量が健常群より高く、その中でも、Streptococcus 属の相対的存在量が他の対象者より有意に高かった（P<0.01）。
また、CL-I group では、Shannon-Weaver 係数も他の対象者より有意に高かった（P<0.01）。
涙液のメタ 16S 解析の結果、AS 群の涙液の菌叢パターンは、CL 保存液同様 3 つのグループに分かれ、CL-I group では、涙液の菌叢に占める Streptococcus 属の相対的存在量も他の対象者より有意に高かった（P<0.01）。AS 群、健常群の全例において、涙液中に Streptococcus 属の菌体に結合する IgE が検出された。

【考察】
培養可能な細菌による CL 保存液の汚染状況は、AS を有する症例と健常者の間で差はなく、過去の報告と一致していた。
AS を有する一部の CL 装用者の CL 保存液・涙液には Streptococcus 属が相対的に多く含まれており、涙液中に Streptococcus 属に結合する IgE が存在していたことから、CL 装用に伴う ASの中には、Streptococcus 属が病因として関与する病型がある可能性が考えられた。

【結論】
CL 装用に伴う AS には、Streptococcus 属が関与している可能性がある。

論文の公開元へ

関連論文

関連論文をもっと見る

参考文献

1. Skotnitsky CC, Naduvilath TJ, Sweeney DF and Sankaridurg PR: Two presentations of contact lens-induced papillary conjuncti- vitis (CLPC) in hydrogel lens wear: Local and general. Optom Vis Sci 83: 27-36, 2006.

2. Solomon A: Allergic manifestations of contact lens wearing. Curr Opin Allergy Clin Immunol 16: 492-497, 2016.

3. Tan ME, Demirci G, Pearce D, Jalbert I, Sankaridurg P and Willcox MD: Contact lens-induced papillary conjunctivitis is associated with increased albumin deposits on extended wear hydrogel lenses. Adv Exp Med Biol 506: 951-955, 2002.

4. Tagliaferri A, Love TE and Szczotka-Flynn LB: Risk factors for contact lens-induced papillary conjunctivitis associated with sili- cone hydrogel contact lens wear. Eye Contact Lens 40: 117-122, 2014.

5. Shin H, Price K, Albert L, Dodick J, Park L and Dominguez-Bello MG: Changes in the eye microbiota associated with contact lens wearing. MBio 7: e00198, 2016.

6. Amann RI, Ludwig W and Schleifer KH: Phylogenetic identifi- cation and in situ detection of indivisual microbial cells without cultivation. Microbiol Rev 59: 143-169, 1995.

7. Human Microbiome Project Consortium: Structure, function and diversity of the healthy human microbiome. Nature 486: 207-214, 2012.

8. Ahmed S, Macfarlane GT, Fite A, McBain AJ, Gilbert P and Macfarlane S: Mucosa-associated bacterial diversity in relation to human terminal ileum and colonic biopsy samples. Appl Environ Microbiol 73: 7435-7442, 2007.

9. Grice EA, Kong HH, Renaud G, Young AC, NISC Comparative Sequencing Program; Bouffard GG, Blakesley RW, Wolfsberg TG, Turner ML and Segre JA: A diversity profile of the human skin microbiota. Genome Res 18: 1043-1050, 2008.

10. Dong Q, Brulc JM, Iovieno A, Bates B, Garoutte A, Miller D, Revanna KV, Gao X, Antonopoulos DA, Slepak VZ and Shestopalov VI: Diversity of bacteria at healthy human conjunc- tiva. Invest Ophthalmol Vis Sci 52: 5408-5413, 2011.

11. Eguchi H, Hotta F, Kuwahara T, Imaohji H, Miyazaki C, Hirose M, Kusaka S, Fukuda M and Shimomura Y: Diagnostic approach to ocular infections using various techniques from conventional culture to next-generation sequencing analysis. Cornea 36 (Suppl 1): S46-S52, 2017.

12. Huang Y, Yang B and Li W: Defining the normal core micro- biome of conjunctival microbial communities. Clin Microbiol Infect 22: 643.e7-643.e12, 2016.

13. Bakhtiar SM, LeBlanc JG, Salvucci E, Ali A, Martin R, Langella P, Chatel JM, Miyoshi A, Bermúdez-Humarán LG and Azevedo V: Implications of the human microbiome in inflam- matory bowel diseases. FEMS Microbiol Lett 342: 10-17, 2013.

14. Turnbaugh PJ, Ley RE, Mahowald MA, Magrini V, Mardis ER and Gordon JI: An obesity-associated gut microbiome with increased capacity for energy harvest. Nature 444: 1027-1031, 2006.

15. Chiacchierini E, Restuccia D and Vinci G: Evaluation of two different extraction methods for chromatographic determination of bioactive amines in tomato products. Talanta 69: 548-555, 2006.

16. Morita H, Kuwahara T, Ohshima K, Sasamoto H, Itoh K, Hattori M, Hayashi T and Takami H: An improved DNA isola- tion method for metagenomic analysis of the microbial flora of the human intestine. Microbes Environ 22: 214-222, 2007.

17. Klindworth A, Pruesse E, Schweer T, Peplies J, Quast C, Horn M and Glöckner FO: Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Res 41: e1, 2013.

18. Aronesty E: Comparison of sequencing utility programs. Open Bioinformatics J 7: 1-8, 2013.

19. Navas-Molina JA, Peralta- Sánchez JM, González A, McMurdie PJ, Vázquez-Baeza Y, Xu Z, Ursell LK, Lauber C, Zhou H, Song SJ, et al: Advancing our understanding of the human microbiome using QIIME. Methods Enzymol 531: 371-444, 2013.

20. Edgar RC: Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26: 2460-2461, 2010.

21. Kanda Y: Investigation of the freely available easy-to-use soft- ware ‘EZR’ for medical statistics. Bone Marrow Transplant 48: 452-458, 2013.

22. Dantam J, McCanna DJ, Subbaraman LN, Papinski D, Lakkis C, Mirza A, Berntsen DA, Morgan P, Nichols JJ and Jones LW: Microbial contamination of contact lens storage cases during daily wear use. Optom Vis Sci 93: 925-932, 2016.

23. Boost MV and Cho P: Microbial flora of tears of orthokeratology patients, and microbial contamination of contact lenses and contact lens accessories. Optom Vis Sci 82: 451-458, 2005.

24. Yung MS, Boost M, Cho P and Yap M: Microbial contamination of contact lenses and lens care accessories of soft contact lens wearers (university students) in Hong Kong. Ophthalmic Physiol Opt 27: 11-21, 2007.

25. Willcox MD, Power KN, Stapleton F, Leitch C, Harmis N and Sweeney DF: Potential sources of bacteria that are isolated from contact lenses during wear. Optom Vis Sci 74: 1030-1038, 1997.

26. Wu YT, Zhu H, Harmis NY, Iskandar SY, Willcox M and Stapleton F: Profile and frequency of microbial contamination of contact lens cases. Optom Vis Sci 87: E152-E158, 2010.

27. Willcox MD, Carnt N, Diec J, Naduvilath T, Evans V, Stapleton F, Iskandar S, Harmis N, de la Jara PL and Holden BA: Contact lens case contamination during daily wear of silicone hydrogels. Optom Vis Sci 87: 456-464, 2010.

28. McLaughlin-Borlace L, Stapleton F, Matheson M and Dart JK: Bacterial biofilm on contact lenses and lens storage cases in wearers with microbial keratitis. J Appl Microbiol 84: 827-838, 1998.

29. Staley JT and Konopka A: Measurement of in situ activities of nonphotosynthetic microorganisms in aquatic and terrestrial habitats. Annu Rev Microbiol 39: 321-346, 1985.

30. Eguchi H, Hotta F, Kuwahara T, Nakayama-Imaohji H, Kusaka S and Shimomura Y: Acute keratoconjunctivitis due to contami- nation of contact lens care solution with histamine-producing Raoultella species. A case report. Medicine (Baltimore) 96: e9310, 2017.

31. Nichols KK: Patient-reported symptoms in dry eye disease. Ocul Surf 4: 137-145, 2006.

32. Belmonte C: Pain, dryness, and itch sensations in eye surface disorders are defined by a balance between inflammation and sensory nerve injury. Cornea 38 (Suppl 1): S11-S24, 2019.

33. Stapleton F, Stretton S, Sankaridurg PR, Chandoha H and Shovlin J: Hypersensitivity responses and contact lens wear. Cont Lens Anterior Eye 26: 57-69, 2003.

34. Zhao Z, Fu H, Skotnitsky CC, Sankaridurg PR and Willcox MD: IgE antibody on worn highly oxygen-permeable silicone hydrogel contact lenses from patients with contact lens-induced papillary conjunctivitis (CLPC). Eye Contact Lens 34: 117-121, 2008.

35. Barishak Y, Zavaro A, Samra Z and Sompolinsky D: An immu- nological study of papillary conjunctivitis due to contact lenses. Curr Eye Res 3: 1161-1168, 1984.

36. Bisgaard H, Hermansen MN, Buchvald F, Loland L, Halkjaer LB, Bønnelykke K, Brasholt M, Heltberg A, Vissing NH, Thorsen SV, et al: Childhood asthma after bacterial colonization of the airway in neonates. N Engl J Med 357: 1487-1495, 2007.

37. Jounio U, Juvonen R, Bloigu A, Silvennoinen-Kassinen S, Kaijalainen T, Kauma H, Peitso A, Saukkoriipi A, Vainio O, Harju T and Leinonen M: Pneumococcal carriage is more common in asthmatic than in non-asthmatic young men. Clin Respir J 4: 222-229, 2010.

38. Teo SM, Mok D, Pham K, Kusel M, Serralha M, Troy N, Holt BJ, Hales BJ, Walker ML, Hollams E, et al: The infant airway micro- biome in health and disease impacts later asthma development. Cell Host Microbe 17: 704-715, 2015.

39. Zhang Q, Cox M, Liang Z, Brinkmann F, Cardenas PA, Duff R, Bhavsar P, Cookson W, Moffatt M and Chung KF: Airway microbiota in severe asthma and relationship to asthma severity and phenotypes. PLoS One 11: e0152724, 2016.

40. Kloepfer KM, Lee WM, Pappas TE, Kang TJ, Vrtis RF, Evans MD, Gangnon RE, Bochkov YA, Jackson DJ, Lemanske RF Jr, et al: Detection of pathogenic bacteria during rhinovirus infection is associated with increased respiratory symptoms and exacerba- tions of asthma. J Allergy Clin Immunol 133: 1301-1307, e1-e3, 2014.

41. Green BJ, Wiriyachaiporn S, Grainge C, Rogers GB, Kehagia V, Lau L, Carroll MP, Bruce KD and Howarth PH: Potentially pathogenic airway bacteria and neutrophilic inflammation in treatment resistant severe asthma. PLoS One 9: e100645, 2014.

42. Weisenseel P and Prinz JC: Incidental detection of S. pyogenes-DNA in psoriatic skin by PCR. Arch Dermatol Res 296: 573-576, 2005.

43. Valdimarsson H, Thorleifsdottir RH, Sigurdardottir SL, Gudjonsson JE and Johnston A: Psoriasis-as an autoimmune disease caused by molecular mimicry. Trends Immunol 30: 494-501, 2009.

44. Prinz JC: Psoriasis vulgaris-a sterile antibacterial skin reaction mediated by cross-reactive T cells? An immunological view of the pathophysiology of psoriasis. Clin Exp Dermatol 26: 326-332, 2001.

45. Gudjonsson JE, Thorarinsson AM, Sigurgeirsson B, Kristinsson KG and Valdimarsson H: Streptococcal throat infec- tions and exacerbation of chronic plaque psoriasis: A prospective study. Br J Dermatol 149: 530-534, 2003.

46. Gray TB, Cursons RT, Sherwan JF and Rose PR: Acanthamoeba, bacterial, and fungal contamination of contact lens storage cases. Br J Ophthalmol 79: 601-605, 1995.

47. Devonshire P, Munro FA, Abernethy C and Clark BJ: Microbial contamination of contact lens cases in the west of Scotland. Br J Ophthalmol 77: 41-45, 1993.

参考文献をもっと見る