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5. Conclusions
Our study shows that the directionality of the microglia-triggered
intrinsic plasticity is inverted between the mPFC L5 pyramidal cells
and the cerebellar Purkinje cells. The induction of the hypoexcitability
plasticity of mPFC L5 pyramidal neurons is mediated by the TNF- α and
is dependent on the intraneuronal activity of protein phosphatases. The
functional upregulation of SK1 channels is involved in the LTD of mPFC
pyramidal cells. The contrasting responses against acute inflammation
in the two brain regions may cause the impairment of network func
tional connectivity during immune-associated brain dysfunctions.
Animal ethics statement
All procedures were performed following the guidelines of the Ani
mal Care and Use Committees and approved by the Ethical Committee of
Kyoto University. All animal handling and reporting comply with the
ARRIVE guidelines. Rats were housed (5 animals at maximum in each
cage) and maintained under a 12-h light: 12-h dark cycle, at a constant
temperature and humidity (20–24 ◦ C, 35%–55%), with food and water
available ad libitum.
Funding sources
This work was supported by grants from the Brain Science
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