リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

リケラボ 全国の大学リポジトリにある学位論文・教授論文を一括検索するならリケラボ論文検索大学・研究所にある論文を検索できる

大学・研究所にある論文を検索できる 「酪農における抗菌性物質使用量調査と使用量低減に関する研究」の論文概要。リケラボ論文検索は、全国の大学リポジトリにある学位論文・教授論文を一括検索できる論文検索サービスです。

コピーが完了しました

URLをコピーしました

論文の公開元へ論文の公開元へ
書き出し

酪農における抗菌性物質使用量調査と使用量低減に関する研究

菊池, 允人 東京大学 DOI:10.15083/0002006904

2023.03.24

概要





















菊池

允人

2015年にWHO総会で採択された薬剤耐性グローバルアクションプランを受け、2016年、日
本でも薬剤耐性アクションプランが施行され、畜産分野でも抗菌性物質の適切な使用が求
められている。そのため現状の抗菌性物質使用量を評価する必要があるが、国内の酪農家
における報告は少ない。乳牛の代表的な疾病である乳房炎は、欧州を中心とした諸外国で
は抗菌性物質の使用量を増加させる主な要因とされ、特に抗菌性物質の使用量を増加させ
る乾乳期の乳房炎治療(dry cow treatment:DCT)が問題視され見直される動きがある。
また、近年では乳房炎予防のためのワクチンが発売され普及し始めており、乳房炎防除に
よる抗菌性物質の使用低減効果が期待されている。本研究は、千葉県の酪農家における抗
菌性物質の使用の実態を調査するとともに、抗菌性物質の使用量低減のための臨床技術お
よび予防法について検討することを目的とした。
本論文は5章から構成され、第1章で研究背景と目的が論じられている。
第2章では千葉県の酪農家で使用された抗菌性物質を、defined daily dose(DDD)値を
用いて集計し評価している。DDD値は医療品の主な適応症の中等症に対して成人(平均体重
70㎏を想定)に1日あたりに用いられると考えられる用量と定義され、医療分野での抗菌性
物質使用量調査のために生まれた概念であるが、近年、欧州各国を中心に動物用DDD値を設
定し、抗菌性物質使用量を評価する取り組みが行われている。本研究では、県内酪農家に
おける抗菌性物質使用状況を2014~2016年の3年間分調査し、製品および投与経路ごとに定
められた日本版DDD値を用いてTreatment Days(平均治療日数)を算出し、解析を行った。
また、近年の農家戸数減少・大規模集約化の傾向を踏まえ、飼養規模ごとの抗菌性物質使
用量の比較を行った。2016年の1年間については、乳房炎原因菌の薬剤耐性との関連も調べ
た。その結果、乳房炎に使用される抗菌性物質、特に乾乳期治療に用いられるペニシリン
のTreatment Daysが最も大きいことが分かった。規模別では成牛飼養頭数30頭未満の小規
模農家よりも30頭以上の中・大規模農家のTreatment Daysが有意に大きかった。また、乳
房炎の原因菌として薬剤耐性菌が検出された農家では、Treatment Daysが有意に大きかっ
た。DDD値を用いた解析により抗菌性物質の種類および投与経路ごとの比較が可能になり、
乳房炎防除の対策をすることで抗菌性物質使用量を低減できる可能性が示唆された。

第3章では、抗菌性物質使用量を増加させる要因の一つである難治性の慢性乳房炎に対す
る新たな治療法について研究している。ある種の細菌は物質や組織の表面に接着すると多
糖類、タンパク質や細胞外DNAなどを産生しながら増殖してバイオフィルムという構造体を
形成するが、Streptococcus uberisや Staphylococcus aureus(S. aureus)などの牛の乳
房炎起因菌は乳房内でこのバイオフィルムを形成し、抗菌性物質や免疫細胞の作用に抵抗
性を示すことで難治化に寄与すると考えられている。本研究では、酪農家で発生した難治
性乳房炎に対して、ショート乾乳(3日間の搾乳休止)と、バイオフィルムの分解効果があ
るとされる高張食塩水による乳房内洗浄とを併用した治療を実施し、高張食塩水の代わり
に生理食塩水を用いた対照群と治療効果を比較した。その結果、試験群では対照群よりも
有意に高い治癒率が得られたことから、本法はバイオフィルム形成の関与が疑われる難治
性乳房炎に対し有効な、新たな治療法であると論じている。
第4章では、大腸菌群による甚急性乳房炎やS. aureusによる慢性乳房炎が多発し問題と
なっていた1酪農家で乳房炎ワクチンを使用し、その効果を検証した。当該農場では大腸菌
群とS. aureusの乳房炎を予防する目的で2017年6月から二価不活化乳房炎ワクチンの接種
を開始した。その結果、2017年度は2015年度と比較してバルク乳の平均体細胞数の有意な
減少、乳房炎の診療回数および診療費用の減少傾向が認められ、重篤化する乳房炎や潜在
性乳房炎が減少したことが示唆された。本ワクチンの投与は、乳房炎による酪農家の損失
を軽減するとともに、抗菌性物質使用量の低減につながる可能性が示唆された。
第 5 章では総合考察が展開されている。第 2 章の調査で酪農においては特に乳房炎が抗
菌性物質使用量を増加させることが示唆された。抗菌性物質使用量の低減のためには本研
究のような解析法による使用量モニタリングの継続が不可欠であり、今後は千葉県に限ら
ず全国的な調査を実施する必要性を論じている。また、調査の結果を踏まえて抗菌性物質
使用量低減を実行するのは畜産農家自身と産業動物臨床に携わる獣医師の責務であり、そ
の実現のためには第 3、4 章で行ったような臨床現場での個体診療技術や予防獣医療の研究
を今後も積み重ねてゆくことが重要であると考察している。
これらの研究成果は、学術上および臨床応用上寄与するところが少なくない。よって、
審査委員一同は本論文が博士(獣医学)の学位論文として価値あるものと認めた。

この論文で使われている画像

参考文献

Atkinson O. Management of transition cows in dairy practice, In Practice 2016; 38:

229-240.

Barkema HW, Schukken YH, Zadoks RN. Invited Review: The role of cow, pathogen,

and treatment regimen in the therapeutic success of bovine Staphylococcus aureus

mastitis. J Dairy Sci. 2006; 89(6): 1877-1895.

Berry EA, Hillerton JE. The effect of selective dry cow treatment on new

intramammary infections. J Dairy Sci. 2002; 85(1): 112-121.

Bohl LP, Isaac P, Breser ML, Orellano MS, Correa SG, de Talamoni NGT, Porporatto

C. Interaction between bovine mammary epithelial cells and planktonic or biofilm

Staphylococcus aureus: The bacterial lifestyle determines its internalization ability

and the pathogen recognition. Microbial Pathogenesis. 2020: 104604.

Boireau C, Cazeau G, Jarrige N, Calavas D, Madec JY, Leblond A, Haenni M, Gay É.

Antimicrobial resistance in bacteria isolated from mastitis in dairy cattle in France,

2006-2016. J Dairy Sci. 2018; 101(10): 9451-9462.

Bradley AJ, Green MJ. The importance of the nonlactating period in the epidemiology

of intramammary infection and strategies for prevention. Vet Clin North Am Food

Anim Pract. 2004; 20(3): 547-568.

94

Bradley AJ, Breen JE, Payne B, White V, Green MJ. An investigation of the efficacy of

a polyvalent mastitis vaccine using different vaccination regimens under field

conditions in the United Kingdom. J Dairy Sci. 2015; 98(3): 1706-1720.

Brunton LA, Duncan D, Coldham NG, Snow LC, Jones JR. A survey of antimicrobial

usage on dairy farms and waste milk feeding practices in England and Wales. Vet Rec.

2012; 171(12): 296.

CDC (Centers for Disease Control and Prevention): Antibiotic Resistance Threats in

the United States, 2013, https://www.cdc.gov/drugresistance/pdf/ar-threats-2013508.pdf

Ceri H, Olson ME, Stremick C, Read RR, Morck D, Buret A. The Calgary Biofilm

Device: new technology for rapid determination of antibiotic susceptibilities of

bacterial biofilms. J Clin Microbiol. 1999; 37(6): 1771-1776.

Chantziaras I, Boyen F, Callens B, Dewulf J. Correlation between veterinary

antimicrobial use and antimicrobial resistance in food-producing animals: a report on

seven countries. J. Antimicrob. Chemother. 2014; 69(3): 827-834.

Chiba A, Sugimoto S, Sato F, Hori S, Mizunoe Y. A refined technique for extraction of

extracellular matrices from bacterial biofilms and its applicability. Microb Biotechnol.

2015; 8(3): 392-403.

95

Chiba K, Miyazaki T, Sekiyama Y, Miyazaki M, Okada K. The therapeutic efficacy of

allyl isothiocyanate in cows with bovine digital dermatitis. J Vet Med Sci. 2017; 79(7):

1191-1195.

Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of

persistent infections. Science. 1999; 284(5418): 1318-1322.

Collado R, Montbrau C, Sitjà M, Prenafeta A. Study of the efficacy of a Streptococcus

uberis mastitis vaccine against an experimental intramammary infection with a

heterologous strain in dairy cows. J Dairy Sci. 2018; 101(11): 10290-10302.

Cooper MA, Shlaes D. Fix the antibiotics pipeline. Nature. 2011 ; 472 (7341): 32.

Costa AR, Henriques M, Oliveira R, Azeredo J. The role of polysaccharide intercellular

adhesin (PIA) in Staphylococcus epidermidis adhesion to host tissues and subsequent

antibiotic tolerance. Eur J Clin Microbiol Infect Dis. 2009; 28(6): 623-629.

Dalecki AG, Crawford CL, Wolschendorf F. Targeting biofilm associated

Staphylococcus aureus using resazurin based drug-susceptibility assay. J Vis Exp.

2016; 111: 53925.

96

Dellit TH, Owens RC, McGowan JE Jr, Gerding DN, Weinstein RA, Burke JP,

Huskins WC, Paterson DL, Fishman NO, Carpenter CF, Brennan PJ, Billeter M,

Hooton TM; Infectious Diseases Society of America; Society for Healthcare

Epidemiology of America. Infectious Diseases Society of America and the Society for

Healthcare Epidemiology of America guidelines for developing an institutional

program to enhance antimicrobial stewardship. Clin Infect Dis. 2007; 44(2): 159-177.

Dieser SA, Fessia AS, Ferrari MP, Raspanti CG, Odierno LM. Streptococcus uberis: In

vitro biofilm production in response to carbohydrates and skim milk. Rev Argent

Microbiol. 2017; 49(4): 305-310.

Dufour S, Dohoo IR, Barkema HW, Descôteaux L, Devries TJ, Reyher KK, Roy JP,

Scholl DT. Manageable risk factors associated with the lactational incidence,

elimination, and prevalence of Staphylococcus aureus intramammary infections in

dairy cows. J Dairy Sci. 2012; 95(3): 1283-1300.

Dunne WM Jr. Bacterial adhesion: seen any good biofilms lately? Clin Microbiol Rev.

2002; 15(2): 155-166.

Duse A, Persson Waller K, Emanuelson U, Ericsson Unnerstad H, Persson Y,

Bengtsson B. Occurrence and spread of quinolone-resistant Escherichia coli on dairy

farms. Appl Environ Microbiol. 2016; 82(13): 3765-3773.

97

EMA (European Medicine Agency), 2015, Principles on assignment of defined daily

dose for animals (DDDvet) and defined course dose for animals (DCDvet).,

http://www.ema.europa.eu/docs/en_GB/document_library/Scientific_guideline/2015/06/

WC500188890.pdf

FDA (Food and Drug Administration), Summary report on antimicrobials cold or

distributed for use in food producing animals., 2015,

https://www.fda.gov/files/about%20fda/published/2015-Summary-Report-onAntimicrobials-Sold-or-Distributed-for-Use-in-Food-Producing-Animals.pdf

Fischbach MA, Walsh CT. Antibiotics for emerging pathogens. Science. 2009;

325(5944): 1089-1093.

Fleming A. On the antibacterial action of cultures of a Penicillium, with special

reference to their use in the isolation of B. influenzæ. Br J Exp Pathol. 1929; 10(3):

226-236.

Franz S, Floek M, Hofmann-Parisot M. Ultrasonography of the bovine udder and teat.

Vet Clin North Am Food Anim Pract. 2009; 25(3): 669-685.

Freick M, Frank Y, Steinert K, Hamedy A, Passarge O, Sobiraj A. Mastitis vaccination

using a commercial polyvalent vaccine or a herd-specific Staphylococcus aureus

vaccine. Results of a controlled field trial on a dairy farm. Tierarztl Prax Ausg G

Grosstiere Nutztiere. 2016; 44(4): 219-229.

98

Gauvain H. Sea bathing in the treatment of surgical tuberculosis. Br Med J. 1935;

2(3909): 1087-1090.

Geoghegan JA, Corrigan RM, Gruszka DT, Speziale P, O'Gara JP, Potts JR, Foster TJ.

Role of surface protein SasG in biofilm formation by Staphylococcus aureus. J

Bacteriol. 2010; 192(21): 5663-5673.

Gomes F, Saavedra MJ, Henriques M. Bovine mastitis disease/pathogenicity: evidence

of the potential role of microbial biofilms. Pathog Dis. 2016; 74(3): ftw006.

González Pereyra V, Pol M, Pastorino F, Herrero A. Quantification of antimicrobial

usage in dairy cows and preweaned calves in Argentina. Prev Vet Med. 2015; 122(3):

273-279.

Government of Japan, 2016. National Action Plan on Antimicrobial Resistance 20162020. https://www.mhlw.go.jp/file/06-Seisakujouhou-10900000Kenkoukyoku/0000138942.pdf

Graham JP, Evans SL, Price LB, Silbergeld EK. Fate of antimicrobial-resistant

enterococci and staphylococci and resistance determinants in stored poultry litter.

Environ Res. 2009; 109(6): 682-689.

99

Gruet P, Maincent P, Berthelot X, Kaltsatos V. Bovine mastitis and intramammary

drug delivery: review and perspectives. Adv Drug Deliv Rev. 2001; 50(3): 245-259.

Guccione J, Pesce A, Pascale M, Salzano C, Tedeschi G, D'Andrea L, De Rosa A,

Ciaramella P. Efficacy of a polyvalent mastitis vaccine against Staphylococcus aureus

on a dairy Mediterranean buffalo farm: results of two clinical field trials. BMC Vet

Res. 2017; 13(1): 29.

Haimerl P, Arlt S, Borchardt S, Heuwieser W. Antibiotic treatment of metritis in dairy

cows-A meta-analysis. J Dairy Sci. 2017; 100(5): 3783-3795.

Halasa T, Huijps K, Østerås O, Hogeveen H. Economic effects of bovine mastitis and

mastitis management: a review. Vet Q. 2007; 29(1): 18-31.

Hand KJ, Godkin A, Kelton DF. Milk production and somatic cell counts: a cow-level

analysis. J Dairy Sci. 2012; 95(3): 1358-1362.

Hao H, Cheng G, Iqbal Z, Ai X, Hussain HI, Huang L, Dai M, Wang Y, Liu Z, Yuan Z.

Benefits and risks of antimicrobial use in food-producing animals. Front Microbiol.

2014; 5: 288.

100

Heider LC, Funk JA, Hoet AE, Meiring RW, Gebreyes WA, Wittum TE. Identification

of Escherichia coli and Salmonella enterica organisms with reduced susceptibility to

ceftriaxone from fecal samples of cows in dairy herds. Am J Vet Res. 2009; 70(3): 389393.

Heinlin J, Schiffner-Rohe J, Schiffner R, Einsele-Krämer B, Landthaler M, Klein A,

Zeman F, Stolz W, Karrer S. A first prospective randomized controlled trial on the

efficacy and safety of synchronous balneophototherapy vs. narrow-band UVB mono

therapy for atopic dermatitis. J Eur Acad Dermatol Venereol. 2011; 25(7): 765-773.

Herago T, Agonafir A. Growth promoters in cattle. Advances in Biological Research.

2017, 11(1): 24-34.

Higgins HM, Dryden IL, Green MJ. A Bayesian elicitation of veterinary beliefs

regarding systemic dry cow therapy: variation and importance for clinical trial design.

Prev Vet Med. 2012; 106(2): 87-96.

Hillerton JE, Kliem KE. Effective treatment of Streptococcus uberis clinical mastitis

to minimize the use of antibiotics. J Dairy Sci. 2002; 85(4): 1009-1014.

HIPRA 社, "HIPRAnews", 2019-1-30,

https://www.hipra.com/portal/en/hipra/about/news/detail/mastitis-prevention-10-years

(参照 2020-12-10)

101

Hockenhull J, Turner AE, Reyher KK, Barrett DC, Jones L, Hinchliffe S, Buller HJ.

Antimicrobial use in food-producing animals: a rapid evidence assessment of

stakeholder practices and beliefs. Vet Rec. 2017; 181(19): 510.

Hogan J, Larry Smith K. Coliform mastitis. Vet Res. 2003; 34(5): 507-519.

Holzhauer M, Bartels CJ, van Barneveld M, Vulders C, Lam T. Curative effect of

topical treatment of digital dermatitis with a gel containing activated copper and zinc

chelate. Vet Rec. 2011; 169(21): 555.

Huang R, Li M, Gregory RL. Bacterial interactions in dental biofilm. Virulence. 2011;

2(5): 435-444.

Hughes P, Heritage J. Antibiotic growth-promoters in food animals. In: FAO Animal

Production and Health Paper. 2004; 129-152.

Huijps K, Lam TJ, Hogeveen H. Costs of mastitis: facts and perception. J Dairy Res.

2008; 75(1): 113-120.

Jaśkiewicz M, Janczura A, Nowicka J, Kamysz W. Methods Used for the Eradication

of Staphylococcal Biofilms. Antibiotics (Basel). 2019; 8(4): 174.

102

Kajiyama S, Tsurumoto T, Osaki M, Yanagihara K, Shindo H. Quantitative analysis of

Staphylococcus epidermidis biofilm on the surface of biomaterial. J Orthop Sci. 2009;

14(6): 769-775.

Kanjanawasee D, Seresirikachorn K, Chitsuthipakorn W, Snidvongs K. Hypertonic

Saline Versus Isotonic Saline Nasal Irrigation: Systematic Review and Meta-analysis.

Am J Rhinol Allergy. 2018; 32(4): 269-279.

Kaplan HB, Greenberg EP. Diffusion of autoinducer is involved in regulation of the

Vibrio fischeri luminescence system. J Bacteriol. 1985; 163(3): 1210-1214.

Kayitsinga J, Schewe RL, Contreras GA, Erskine RJ. Antimicrobial treatment of

clinical mastitis in the eastern United States: The influence of dairy farmers' mastitis

management and treatment behavior and attitudes. J Dairy Sci. 2017; 100(2):13881407.

Keane OM. Symposium review: Intramammary infections-Major pathogens and

strain-associated complexity. J Dairy Sci. 2019; 102(5): 4713-4726.

Knapp CW, Dolfing J, Ehlert PA, Graham DW. Evidence of increasing antibiotic

resistance gene abundances in archived soils since 1940. Environ Sci Technol. 2010;

44(2): 580-587.

103

Lam TJ, van den Borne BH, Jansen J, Huijps K, van Veersen JC, van Schaik G,

Hogeveen H. Improving bovine udder health: a national mastitis control program in

the Netherlands. J Dairy Sci. 2013; 96(2): 1301-1311.

Landin H, Mörk MJ, Larsson M, Waller KP. Vaccination against Staphylococcus

aureus mastitis in two Swedish dairy herds. Acta Vet Scand. 2015; 57: 81.

Larsen J, Clasen J, Hansen JE, Paulander W, Petersen A, Larsen AR, Frees D.

Copresence of tet(K) and tet(M) in livestock-associated Methicillin-resistant

Staphylococcus aureus clonal complex 398 is associated with increased fitness during

exposure to sublethal concentrations of Tetracycline. Antimicrob Agents Chemother.

2016; 60(7): 4401-4403.

Laven RA, Hunt H. Evaluation of copper sulphate, formalin and peracetic acid in

footbaths for the treatment of digital dermatitis in cattle. Vet Rec. 2002; 151(5): 144146.

Lawn JE, Cousens S, Zupan J; Lancet Neonatal Survival Steering Team. 4 million

neonatal deaths: when? Where? Why? Lancet. 2005; 365(9462): 891-900.

104

Laxminarayan R, Duse A, Wattal C, Zaidi AK, Wertheim HF, Sumpradit N, Vlieghe E,

Hara GL, Gould IM, Goossens H, Greko C, So AD, Bigdeli M, Tomson G, Woodhouse

W, Ombaka E, Peralta AQ, Qamar FN, Mir F, Kariuki S, Bhutta ZA, Coates A,

Bergstrom R, Wright GD, Brown ED, Cars O. Antibiotic resistance-the need for global

solutions. Lancet Infect Dis. 2013; 13(12): 1057-1098.

Lewis K. Multidrug tolerance of biofilms and persister cells. Curr Top Microbiol

Immunol. 2008; 322: 107-131.

Lhermie G, Tauer LW, Gröhn YT. The farm cost of decreasing antimicrobial use in

dairy production. PLoS One. 2018; 13(3): e0194832.

Loo E, Lai KS, Mansor R, Antimicrobial usage and resistance in dairy cattle

production, 2020, DOI: 10.5772/intechopen.81365

Mahmmod YS, Klaas IC, Svennesen L, Pedersen K, Ingmer H. Communications of

Staphylococcus aureus and non-aureus Staphylococcus species from bovine

intramammary infections and teat apex colonization. J Dairy Sci. 2018; 101(8): 73227333.

Mann S, McArt J, Abuelo A, Production-related metabolic disorders of cattle: ketosis,

milk fever and grass staggers, In Practice 2019; 41: 205-219.

105

Merritt JH, Kadouri DE, O'Toole GA. Growing and analyzing static biofilms. Curr

Protoc Microbiol. 2005; Chapter 1: Unit 1B.1.

Molina-Manso D, del Prado G, Ortiz-Pérez A, Manrubia-Cobo M, Gómez-Barrena E,

Cordero-Ampuero J, Esteban J. In vitro susceptibility to antibiotics of staphylococci in

biofilms isolated from orthopaedic infections. Int J Antimicrob Agents. 2013; 41(6):

521-523.

Morimoto K, Shimizu M, Kurose T, Nakatani K, Akita S, Shinozuka Y, Isobe N.

Efficacy of enterotoxigenic Escherichia coli vaccine for bovine clinical mastitis. J Dairy

Res. 2011; 78(2): 149-153.

Mullard A. Pharmaceutical firms commit US$1 billion to antibiotic development. Nat

Rev Drug Discov. 2020; 19(9): 575-576.

Niemi RE, Vilar MJ, Dohoo IR, Hovinen M, Simojoki H, Rajala-Schultz PJ. Antibiotic

dry cow therapy, somatic cell count, and milk production: Retrospective analysis of the

associations in dairy herd recording data using multilevel growth models. Prev Vet

Med. 2020; 180: 105028.

Nobrega DB, De Buck J, Barkema HW. Antimicrobial resistance in non-aureus

staphylococci isolated from milk is associated with systemic but not intramammary

administration of antimicrobials in dairy cattle. J Dairy Sci. 2018; 101(8): 7425-7436.

106

Okabe T, Shimizu H, Kurihara E, Matsui T, Watanabe T, Haneishi T, Takagi N,

Kikuchi M, Fujimoto Y, Yamazaki S. Investigation of antibiotics uses in dairy farms in

Chiba Prefecture for a national action plan on antimicrobial resistance. World

Buiatrics Congress 2018

Oliver SP, Murinda SE, Jayarao BM. Impact of antibiotic use in adult dairy cows on

antimicrobial resistance of veterinary and human pathogens: a comprehensive review.

Foodborne Pathog Dis. 2011; 8(3): 337-355.

Oliver SP, Murinda SE. Antimicrobial resistance of mastitis pathogens. Vet Clin North

Am Food Anim Pract. 2012; 28(2): 165-185.

O'Neill J: Tackling drug-resistant infections globally: final report and

recommendations, 2016, https://amrreview.org/sites/default/files/160525_Final%20paper_with%20cover.pdf

O'Toole GA. Microtiter dish biofilm formation assay. J Vis Exp. 2011; 47: 2437.

Otto M. Staphylococcal biofilms. Curr Top Microbiol Immunol. 2008; 322: 207-228.

Pedersen K. Method for studying microbial biofilms in flowing-water systems. Applied

and environmental microbiology. 1982; 43(1): 6–13.

107

Pérez MM, Prenafeta A, Valle J, Penadés J, Rota C, Solano C, Marco J, Grilló MJ,

Lasa I, Irache JM, Maira-Litran T, Jiménez-Barbero J, Costa L, Pier GB, de Andrés D,

Amorena B. Protection from Staphylococcus aureus mastitis associated with poly-Nacetyl beta-1, 6 glucosamine specific antibody production using biofilm-embedded

bacteria. Vaccine. 2009; 27(17): 2379-2386.

Petersson-Wolfe CS., Mullarky IK, Jones GM. "Staphylococcus aureus mastitis: cause,

detection, and control.", vtechworks.lib.vt.edu, 2010.

Pol M, Ruegg PL. Treatment practices and quantification of antimicrobial drug usage

in conventional and organic dairy farms in Wisconsin. J Dairy Sci. 2007; 90(1): 249261.

Postma M, Sjölund M, Collineau L, Lösken S, Stärk KD, Dewulf J; MINAPIG

Consortium. Assigning defined daily doses animal: a European multi-country

experience for antimicrobial products authorized for usage in pigs. J Antimicrob

Chemother. 2015; 70(1): 294-302.

Prenafeta A, March R, Foix A, Casals I, Costa L. Study of the humoral immunological

response after vaccination with a Staphylococcus aureus biofilm-embedded bacterin in

dairy cows: possible role of the exopolysaccharide specific antibody production in the

protection from Staphylococcus aureus induced mastitis. Vet Immunol Immunopathol.

2010; 134(3-4): 208-217.

108

Price LB, Johnson E, Vailes R, Silbergeld E. Fluoroquinolone-resistant Campylobacter

isolates from conventional and antibiotic-free chicken products. Environ Health

Perspect. 2005; 113(5): 557-560.

Saini V, McClure JT, Scholl DT, DeVries TJ, Barkema HW. Herd-level association

between antimicrobial use and antimicrobial resistance in bovine mastitis

Staphylococcus aureus isolates on Canadian dairy farms. J Dairy Sci. 2012; 95(4):

1921-1929.

Saini V, McClure JT, Scholl DT, DeVries TJ, Barkema HW. Herd-level relationship

between antimicrobial use and presence or absence of antimicrobial resistance in

gram-negative bovine mastitis pathogens on Canadian dairy farms. J Dairy Sci. 2013;

96(8): 4965-4976.

Scherpenzeel CGM, den Uijl IEM, van Schaik G, Riekerink RGMO, Hogeveen H, Lam

TJGM. Effect of different scenarios for selective dry-cow therapy on udder health,

antimicrobial usage, and economics. J Dairy Sci. 2016; 99(5): 3753-3764.

Schönborn S, Krömker V. Detection of the biofilm component polysaccharide

intercellular adhesin in Staphylococcus aureus infected cow udders. Vet Microbiol.

2016; 196: 126-128.

109

Schukken YH, Bronzo V, Locatelli C, Pollera C, Rota N, Casula A, Testa F,

Scaccabarozzi L, March R, Zalduendo D, Guix R, Moroni P. Efficacy of vaccination on

Staphylococcus aureus and coagulase-negative staphylococci intramammary infection

dynamics in 2 dairy herds. J Dairy Sci. 2014; 97(8): 5250-5264.

SDA (The Netherlands Veterinary Medicines Institute), 2019, Usage of Antibiotics in

Agricultural Livestock in the Netherlands in 2018., https://cdn.ipulse.nl/autoriteitdiergeneesmiddelen/userfiles/Publications/2018-def-rapport1.pdf

SDA(The Netherlands Veterinary Medicines Institute, 2014, Dutch Veterinary

Medicines Authority; Usage of antibiotics in agricultural livestock in the Netherlands

in 2013, http://www.autoriteitdiergeneesmiddelen.nl/en/publications

Seale AC, Gordon NC, Islam J, Peacock SJ, Scott JAG. AMR Surveillance in low and

middle-income settings - A roadmap for participation in the Global Antimicrobial

Surveillance System (GLASS). Wellcome Open Res. 2017; 2: 92.

Sears PM, McCarthy KK. Management and treatment of staphylococcal mastitis. The

Veterinary Clinics of North America. Food Animal Practice. 2003; 19(1): 171-185.

Seegers H, Fourichon C, Beaudeau F. Production effects related to mastitis and

mastitis economics in dairy cattle herds. Vet Res. 2003; 34(5): 475-491.

110

Shinozuka Y, Hirata H, Ishibashi I, Okawa Y, Kasuga A, Takagi M, Taura Y.

Therapeutic efficacy of mammary irrigation regimen in dairy cattle diagnosed with

acutE. coliform mastitis. J Vet Med Sci. 2009; 71(3): 269-273.

Singh R, Ray P. Quorum sensing-mediated regulation of staphylococcal virulence and

antibiotic resistance. Future Microbiol. 2014; 9(5): 669-681.

Smith TC, Gebreyes WA, Abley MJ, Harper AL, Forshey BM, Male MJ, Martin HW,

Molla BZ, Sreevatsan S, Thakur S, Thiruvengadam M, Davies PR. Methicillinresistant Staphylococcus aureus in pigs and farm workers on conventional and

antibiotic-free swine farms in the USA. PLoS One. 2013; 8(5): e63704.

Smith KL, Todhunter DA, Schoenberger PS. Environmental mastitis: cause,

prevalence, prevention. J Dairy Sci. 1985; 68(6): 1531-1553.

Stevens M, Piepers S, Supré K, Dewulf J, De Vliegher S. Quantification of

antimicrobial consumption in adult cattle on dairy herds in Flanders, Belgium, and

associations with udder health, milk quality, and production performance. J Dairy Sci.

2016; 99(3): 2118-2130.

Swinkels JM, Hilkens A, Zoche-Golob V, Krömker V, Buddiger M, Jansen J, Lam TJ.

Social influences on the duration of antibiotic treatment of clinical mastitis in dairy

cows. J Dairy Sci. 2015; 98(4): 2369-2380.

111

Tang X, Flint SH, Bennett RJ, Brooks JD, Morton RH. Biofilm growth of individual

and dual strains of Klebsiella oxytoca from the dairy industry on ultrafiltration

membranes. J Ind Microbiol Biotechnol. 2009; 36(12): 1491-1497.

Todhunter DA, Smith KL, Hogan JS. Environmental streptococcal intramammary

infections of the bovine mammary gland. J Dairy Sci. 1995; 78(11): 2366-2374.

Tsukatani T, Kawaguchi T, Suenaga H, Shiga M, Ikegami T, Rapid and simple

determination of minimum biofilm eradication concentration by a colorimetric

microbial viability assay based on reduction of a water-soluble tetrazolium salt and

combined effect of antibiotics against microbial biofilm. J. Microbiol. Biotechnol. Food

Sci., 2016; 6: 677-680.

USDA (United States Department of Agriculture), 2008, Dairy 2007. Part III:

Reference of Dairy Cattle Health and Management Practices in the United States,

https://naldc.nal.usda.gov/download/46188/PDF

Van Boeckel TP, Brower C, Gilbert M, Grenfell BT, Levin SA, Robinson TP, Teillant A,

Laxminarayan R. Global trends in antimicrobial use in food animals. Proc Natl Acad

Sci U S A. 2015; 112(18): 5649-5654.

112

van Duijkeren E, Ikawaty R, Broekhuizen-Stins MJ, Jansen MD, Spalburg EC, de

Neeling AJ, Allaart JG, van Nes A, Wagenaar JA, Fluit AC. Transmission of

methicillin-resistant Staphylococcus aureus strains between different kinds of pig

farms. Vet Microbiol. 2008; 126(4): 383-389.

Viswanathan R, Singh AK, Ghosh C, Dasgupta S, Mukherjee S, Basu S. Profile of

neonatal septicaemia at a district-level sick newborn care unit. J Health Popul Nutr.

2012; 30(1): 41-48.

Wagner S, Erskine R, 2013, Antimicrobial drug use in mastitis. In Antimicrobial

Therapy in Veterinary Medicine, https://doi.org/10.1002/9781118675014.ch30.

Waters D, Jawad I, Ahmad A, Lukšić I, Nair H, Zgaga L, Theodoratou E, Rudan I,

Zaidi AK, Campbell H. Aetiology of community-acquired neonatal sepsis in low and

middle income countries. J Glob Health. 2011; 1(2): 154-170.

WHO (World Health Organization): Global priority list of antibiotic-resistant bacteria

to guide research, discovery, and development of new antibiotics, 2017,

https://www.who.int/medicines/publications/WHO-PPL-Short_Summary_25FebET_NM_WHO.pdf?ua=1

Wilson-Welder JH, Alt DP, Nally JE. Digital dermatitis in cattle: Current bacterial

and immunological findings. Animals (Basel). 2015; 5(4): 1114-1135.

113

You Y, Silbergeld EK. Learning from agriculture: understanding low-dose

antimicrobials as drivers of resistome expansion. Front Microbiol. 2014; 5: 284.

Yu ZN, Wang J, Ho H, Wang YT, Huang SN, Han RW. Prevalence and antimicrobialresistance phenotypes and genotypes of Escherichia coli isolated from raw milk

samples from mastitis cases in four regions of China. J Glob Antimicrob Resist. 2020;

22: 94-101.

Yu L, Li W, Zhang M, Cui Y, Chen X, Ni J, Yu L, Shang F, Xue T. Imidazole decreases

the ampicillin resistance of an Escherichia coli strain isolated from a cow with

mastitis by inhibiting the function of autoinducer 2. J Dairy Sci. 2018; 101(4): 33563362.

Zhanel GG, Wiebe R, Dilay L, Thomson K, Rubinstein E, Hoban DJ, Noreddin AM,

Karlowsky JA. Comparative review of the carbapenems. Drugs. 2007; 67(7): 10271052.

厚生労働省, "薬剤耐性ワンヘルス動向調査年次報告 2019", 2020-11-5,

https://www.mhlw.go.jp/content/10906000/000691722.pdf(参照 2020-12-10)

近藤寧子, 松井崇, 三浦道三郎ら.Streptococcus 乳房炎に対する泌乳期ショート乾乳の

効果.家畜衛生学会誌 2013; 39(3): 130-131.

篠塚康典.大腸菌性乳房炎にどう対峙するか.家畜感染症学会誌 2015; 4(4): 179-186.

114

杉浦勝明.2015; 動物用抗菌剤の使用量調査に関する情報整備.

田中秀和, 井上宣子, 菅原久枝.慢性乳房炎(Streptococcus uberis 感染症)に対するショ

ート乾乳治療の取り組み.臨床獣医 2017; 35(6): 12-18.

田村 豊.食用動物に由来する薬剤耐性菌の現状と対策.環境感染誌 2017; 32 (6): 322329.

津谷喜一郎, 五十嵐中, 森川馨.ATC/DDD とは何か 医薬品の合理的使用を目指すものさ

し.薬剤疫学 2004; 9(2): 53-58.

中村友彦, 菊池允人, 田中秀和.管内 1 酪農家における乳質改善の取り組み.千葉県農業

共済組合紫葉会会誌 2014; 59: 52-56.

農林水産省 "畜産物生産における動物用抗菌性物質製剤の慎重使用に関する基本的な考え

方" 2013-12-24, https://www.maff.go.jp/j/syouan/tikusui/yakuzi/pdf/prudent_use.pdf

(参照 2020-12-10)

農林水産省 "畜産統計調査" 2016-10-21,

https://www.maff.go.jp/j/tokei/kouhyou/tikusan/(参照 2020-12-10)

115

農林水産省 "各種抗生物質・合成抗菌剤・駆虫剤・抗原虫剤の販売高と販売量(2018)"

https://www.maff.go.jp/nval/iyakutou/hanbaidaka/pdf/h30_hanbaidaka.pdf(参照

2020-12-10)

農林水産省 "本格的議論のための酪農・乳業の課題" 2019-10-10(2019a)

https://www.maff.go.jp/j/council/seisaku/tikusan/attach/pdf/siryou191010-17.pdf(参照

2020-12-10)

農林水産省 "畜産統計調査" 2019-11-13(2019b)

https://www.maff.go.jp/j/tokei/kouhyou/tikusan/(参照 2020-12-10)

農林水産省 "農業共済制度の概要" (2019c)

https://www.maff.go.jp/j/council/seisaku/kyosai/bukai/r010527/attach/pdf/index-14.pdf

(参照 2020-12-10)

萩原慎一郎.乳房炎ワクチン一斉接種における効果の検討.家畜診療 2020; 67(12): 691695.

藤本恭子, 清水秀茂, 菊池允人, 松井崇, 伊藤貢, 橋本信一郎, 川原史也, 矢原芳博, 杉浦

勝明.牛用および鶏用抗菌剤 ID リスト作成および DDD 値設定の試み.日獣会誌(査読

中)

松浦優, 鮎川理, 近藤寧子, 大谷夏輝, 菊池允人, 田中秀和.子牛の難治性下痢症における

糞便微生物移植の試み.家畜診療 2019; 66(1): 27-33.

116

松田真理, Kwan NCL, 川西路子, 小池良治, 杉浦勝明.日本における家畜バイオマス重

量あたりの抗菌剤使用量の評価:細井らの方法と EU の方法による評価結果の比較.家

畜衛生学雑誌 2017; 42(4): 191-197.

水之江義充.院内感染とバイオフィルム.耳鼻展望 2013; 56(4): 199-203.

117

...

参考文献をもっと見る

全国の大学の
卒論・修論・学位論文

一発検索!

この論文の関連論文を見る