1.
2.
3.
4.
5.
6.
7.
8.
9.
Im, G.I. Biomaterials in orthopaedics: The past and future with immune modulation. Biomater. Res. 2020, 24, 7. [CrossRef]
[PubMed]
Wang, G.; Zreiqat, H. Functional Coatings or Films for Hard-Tissue Applications. Materials 2010, 3, 3994–4050. [CrossRef]
Qin, L.; Yao, S.; Zhao, J.; Zhou, C.; Oates, T.W.; Weir, M.D.; Wu, J.; Xu, H.H.K. Review on Development and Dental Applications
of Polyetheretherketone-Based Biomaterials and Restorations. Materials 2021, 14, 408. [CrossRef]
Kohli, N.; Ho, S.; Brown, S.J.; Sawadkar, P.; Sharma, V.; Snow, M.; García-Gareta, E. Bone remodelling in vitro: Where are we
headed?: -A review on the current understanding of physiological bone remodelling and inflammation and the strategies for
testing biomaterials in vitro. Bone 2018, 110, 38–46. [CrossRef]
Czekanska, E.M.; Stoddart, M.J.; Ralphs, J.R.; Richards, R.G.; Hayes, J.S. A phenotypic comparison of osteoblast cell lines versus
human primary osteoblasts for biomaterials testing. J. Biomed. Mater. Res. A 2014, 102, 2636–2643. [CrossRef]
Czekanska, E.M.; Stoddart, M.J.; Richards, R.G.; Hayes, J.S. In search of an osteoblast cell model for in vitro research. Eur. Cell Mater.
2012, 24, 1–17. [CrossRef]
Hinoi, E.; Fujimori, S.; Takemori, A.; Yoneda, Y. Cell death by pyruvate deficiency in proliferative cultured calvarial osteoblasts.
Biochem. Biophys. Res. Commun. 2002, 294, 1177–1183. [CrossRef]
Coelho, M.J.; Cabral, A.T.; Fernande, M.H. Human bone cell cultures in biocompatibility testing. Part I: Osteoblastic differentiation
of serially passaged human bone marrow cells cultured in alpha-MEM and in DMEM. Biomaterials 2000, 21, 1087–1094. [CrossRef]
Brzezinska,
O.; Łukasik, Z.; Makowska, J.; Walczak, K. Role of Vitamin C in Osteoporosis Development and Treatment-A
Literature Review. Nutrients 2020, 12, 2394. [CrossRef] [PubMed]
Int. J. Mol. Sci. 2021, 22, 7752
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29.
30.
31.
32.
33.
34.
35.
36.
37.
12 of 12
Chin, K.Y.; Ima-Nirwana, S. Vitamin C and Bone Health: Evidence from Cell, Animal and Human Studies. Curr. Drug Targets
2018, 19, 439–450. [CrossRef]
Jonason, J.H.; O’Keefe, R.J. Isolation and culture of neonatal mouse calvarial osteoblasts. Methods Mol. Biol. 2014, 1130, 295–305.
[CrossRef]
Bakker, A.D.; Klein-Nulend, J. Osteoblast isolation from murine calvaria and long bones. Methods Mol. Biol. 2012, 816, 19–29.
[CrossRef]
Orriss, I.R.; Taylor, S.E.; Arnett, T.R. Rat osteoblast cultures. Methods Mol. Biol. 2012, 816, 31–41. [CrossRef]
Doolittle, M.L.; Ackert-Bicknell, C.L.; Jonason, J.H. Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts. Methods Mol. Biol.
2021, 2230, 425–436. [CrossRef] [PubMed]
Kodama, H.; Amagai, Y.; Sudo, H.; Kasai, S.; Yamamoto, S. Establishment of a clonal osteogenic cell line from newborn mouse
calvaria. Jpn. J. Oral Biol. 1981, 23, 899–901. [CrossRef]
Hiura, K.; Sumitani, K.; Kawata, T.; Higashino, K.; Okawa, M.; Sato, T.; Hakeda, Y.; Kumegawa, M. Mouse osteoblastic cells
(MC3T3-E1) at different stages of differentiation have opposite effects on osteoclastic cell formation. Endocrinology 1991, 128,
1630–1637. [CrossRef]
Zhou, H.Y.; Takita, H.; Fujisawa, R.; Mizuno, M.; Kuboki, Y. Stimulation by bone sialoprotein of calcification in osteoblast-like
MC3T3-E1 cells. Calcif. Tissue Int. 1995, 56, 403–407. [CrossRef]
Wang, D.; Christensen, K.; Chawla, K.; Xiao, G.; Krebsbach, P.H.; Franceschi, R.T. Isolation and characterization of MC3T3-E1
preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential. J. Bone Miner. Res. 1999, 14,
893–903. [CrossRef] [PubMed]
Dillon, J.P.; Waring-Green, V.J.; Taylor, A.M.; Wilson, P.J.; Birch, M.; Gartland, A.; Gallagher, J.A. Primary human osteoblast
cultures. Methods Mol. Biol. 2012, 816, 3–18. [CrossRef]
Bilousova, G.; Jun, d.H.; King, K.B.; De Langhe, S.; Chick, W.S.; Torchia, E.C.; Chow, K.S.; Klemm, D.J.; Roop, D.R.; Majka, S.M.
Osteoblasts derived from induced pluripotent stem cells form calcified structures in scaffolds both in vitro and in vivo. Stem. Cells
2011, 29, 206–216. [CrossRef]
Testing Method for Biocompatibility of Implantable Metals Using Cultured Cells 2000. Available online: https://www.jisc.go.jp/
app/jis/general/GnrJISSearch.html (accessed on 25 April 2021).
Takamizawa, S.; Maehata, Y.; Imai, K.; Senoo, H.; Sato, S.; Hata, R. Effects of ascorbic acid and ascorbic acid 2-phosphate,
a long-acting vitamin C derivative, on the proliferation and differentiation of human osteoblast-like cells. Cell Biol. Int. 2004, 28,
255–265. [CrossRef] [PubMed]
Roach, H.I.; Hillier, K.; Shearer, J.R. Stability of ascorbic acid and uptake of the vitamin by embryonic chick femurs during
long-term culture. Biochim. Biophys. Acta 1985, 842, 133–138. [CrossRef]
Feng, J.; Melcher, A.H.; Brunette, D.M.; Moe, H.K. Determination of L-ascorbic acid levels in culture medium: Concentrations in
commercial media and maintenance of levels under conditions of organ culture. In Vitro 1977, 13, 91–99. [CrossRef] [PubMed]
Harada, S.; Matsumoto, T.; Ogata, E. Role of ascorbic acid in the regulation of proliferation in osteoblast-like MC3T3-E1 cells.
J. Bone Miner. Res. 1991, 6, 903–908. [CrossRef] [PubMed]
Sudo, H.; Kodama, H.A.; Amagai, Y.; Yamamoto, S.; Kasai, S. In vitro differentiation and calcification in a new clonal osteogenic
cell line derived from newborn mouse calvaria. J. Cell Biol. 1983, 96, 191–198. [CrossRef] [PubMed]
Orriss, I.R.; Hajjawi, M.O.; Huesa, C.; MacRae, V.E.; Arnett, T.R. Optimisation of the differing conditions required for bone
formation in vitro by primary osteoblasts from mice and rats. Int. J. Mol. Med. 2014, 34, 1201–1208. [CrossRef] [PubMed]
Beck, G.R.; Sullivan, E.C.; Moran, E.; Zerler, B. Relationship between alkaline phosphatase levels, osteopontin expression,
and mineralization in differentiating MC3T3-E1 osteoblasts. J. Cell Biochem. 1998, 68, 269–280. [CrossRef]
Franceschi, R.T.; Iyer, B.S. Relationship between collagen synthesis and expression of the osteoblast phenotype in MC3T3-E1 cells.
J. Bone Miner. Res. 1992, 7, 235–246. [CrossRef]
Khan, M.R.; Mordan, N.; Parkar, M.; Salih, V.; Donos, N.; Brett, P.M. Atypical Mesenchymal Stromal Cell Responses to Topographic
Modifications of Titanium Biomaterials Indicate Cytoskeletal- and Genetic Plasticity-Based Heterogeneity of Cells. Stem Cells Int.
2019, 2019, 5214501. [CrossRef]
Qutob, S.; Dixon, S.J.; Wilson, J.X. Insulin stimulates vitamin C recycling and ascorbate accumulation in osteoblastic cells.
Endocrinology 1998, 139, 51–56. [CrossRef]
Wells, W.W.; Xu, D.P. Dehydroascorbate reduction. J. Bioenerg. Biomembr. 1994, 26, 369–377. [CrossRef] [PubMed]
Noctor, G.; Foyer, C.H. ASCORBATE AND GLUTATHIONE: Keeping Active Oxygen Under Control. Annu. Rev. Plant Physiol.
Plant Mol. Biol. 1998, 49, 249–279. [CrossRef] [PubMed]
Hata, R.; Senoo, H. L-ascorbic acid 2-phosphate stimulates collagen accumulation, cell proliferation, and formation of a threedimensional tissuelike substance by skin fibroblasts. J. Cell Physiol. 1989, 138, 8–16. [CrossRef]
Huang, B.; Wang, Y.; Wang, W.; Chen, J.; Lai, P.; Liu, Z.; Yan, B.; Xu, S.; Zhang, Z.; Zeng, C.; et al. mTORC1 Prevents Preosteoblast
Differentiation through the Notch Signaling Pathway. PLoS Genet. 2015, 11, e1005426. [CrossRef] [PubMed]
Miyazaki, T.; Miyauchi, S.; Tawada, A.; Anada, T.; Matsuzaka, S.; Suzuki, O. Oversulfated chondroitin sulfate-E binds to BMP-4
and enhances osteoblast differentiation. J. Cell Physiol. 2008, 217, 769–777. [CrossRef]
Livak, K.J.; Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta
C(T)) Method. Methods 2001, 25, 402–408. [CrossRef]
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