小児難治性疾患に対する再生医療を用いた新規治療の開発 : 主に脱落乳歯幹細胞に着目して

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田口智章 厚生労働科学研究費補助金難治性疾患等政策研究事業 小児期からの希少難治性消化管疾患の移

行期を包含するガイドラインの確立に関する研究（H26-難治等（難）一般-045）平成 26-28 年度 総括・分

担研究報告書．

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Yamaza T, Akiyama K, Chen C, Liu Y, Shi Y, Gronthos S, Wang S and Shi S. Immunomodulatory properties of

stem cells from human exfoliated deciduous teeth. Stem Cell Res Ther 1 : 5, 2010.

Ma L, Makno Y, Yamaza H, Akiyama K, Hoshino, Y, Song G, Kukita T, Nonaka K, Shi S and Yamaza T :

Cryopreserved dental pulp tissues of exfoliated deciduous teeth is a feasible stem cell resource for

regenerative medicine. PLoS One. 7 : e51777, 2012.

Yamaza T, Alatas FS, Yuniartha R, Yamaza H, Fujiyoshi JK, Yanagi Y, Yoshimaru K, Hayashida M, Matsuura

T, Aijima R, Ihara K, Ohga S, Shi S, Nonaka K and Taguchi T : In vivo hepatogenic capacity and therapeutic

potential of stem cells from human exfoliated deciduous teeth in liver fibrosis in mice. Stem Cell Res Ther. 6 :

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Taguchi T, Ieiri S, Miyoshi K, Kohashi K, Oda Y, Kubota A, Watanabe Y, Matsufuji H, Fukuzawa M and

Tomomasa T : The incidence and outcome of allied disorders of Hirschsprungʼs disease in Japan : Results from

a nationwide survey. Asian J Surg. 40 : 29-34, 2017.

Enosawa S, Horikawa R, Yamamoto A, Sakamoto S, Shigeta T, Nosaka S, Fujimoto J, Nakazawa A, Tanoue A,

Nakamura K, Umezawa A, Matsubara Y, Matsui A and Kasahara M : Hepatocyte transplantation using a

living donor reduced graft in a baby with ornithine transcarbamylase deficiency : A novel source of

hepatocytes. Liver Transplant 20 : 391-393, 2014.

Fujiyoshi J, Yamaza H, Sonoda S, Yuniartha R, Ihara K, Nonaka K, Taguchi T, Ohga S and Yamaza T :

Therapeutic potential of hepatocyte-like-cells converted from stem cells from human exfoliated deciduous

teeth in fulminant Wilsonʼs disease. Sci Rep. 9 : 1535, 2019.

Iwanaka T, Yamaza T, Sonoda S, Yoshimaru K, Matsuura T, Yamaza H, Ohga S, Oda Y and Taguchi T : A

model study for the manufacture and validation of clinical-grade deciduous dental pulp stem cells for chronic

liver fibrosis treatment. Stem Cell Res Ther., published on line, 2020.

Takahashi Y, Yuniartha R, Yamaza T, Sonoda S, Yamaza H, Kirino K, Yoshimaru K, Matsuura T and Taguchi

T : Therapeutic potential of spheroids of stem cells from human exfoliated deciduous teeth for chronic liver

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ほか７名

Takebe T, Sekine K, Enomura M, Koike H, Kimura M, Ogaeri T, Zhang RR, Ueno Y, Zheng YW, Koike N,

Aoyama S, Adachi Y and Taniguchi H : Vascularized and functional human liver from an iPSC-driver organ

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Sakai Y, Yamanouchi K, Ohashi K, Koike M, Utoh R, Hasegawa H, Muraoka I, Suematsu T, Soyama A, Hidaka

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長船健二：iPS 細胞による再生と臓器移植，移植 52：1-6，2017．

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（特に重要な文献については，番号をゴシック体で表記している．

著者プロフィール

田口 智章（たぐち ともあき）

学校法人福岡学園福岡医療短期大学学長 九州大学名誉教授（医学博士）

◆略歴 1954 年 大分県臼杵市に生まれる．1979 年 九州大学医学部医学科卒

1985 年 九州大学大学院医学研究科修了（医学博士）

1987-88 年 カナダ国マックギル大学病理・モントリオール小児病院外科留学

1997 年 米国ピッツバーグ大学小児病院移植外科留学

1998 年 九州大学病院周産母子センター助教授

2006 年 九州大学大学院小児外科学分野教授

2006-8 年，10-12 年，14-18 年 九州大学病院小児医療センター長

2007-9 年，13-15 年，19-20 年 九州大学病院総合周産期母子医療センター長

2015-20 年 九州大学環境発達医学研究センター長

2018-20 年 九州大学病院救命救急センター長・集中治療部長

2020 年 学校法人福岡学園福岡医療短期大学学長

◆研究テーマと抱負 手術だけでは治癒が困難な小児外科疾患の抽出，診断基準やガイドラインの

作成，さらに SHED を中心とした幹細胞をもちいた再生医療による新規治療の開発．小児

外科の高度医療の恩恵が受けられないアジアの開発国における手術支援と現地の小児外科

医の教育．特に小児の肝移植と小児がんの治療を中心にミャンマー，カンボジア，ベトナ

ムのゴールデントライアングルにジャパンハートと連携し，口腔ケアも可能なドリーム

チームで臨む．

◆趣味 山歩き，手術，歴史考証

乳歯幹細胞による小児難治性疾患の治療開発

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Development of New Treatment in Pediatric Intractable

Disease using Regenerative Medicine

−Focusing on Stem Cells from Human Exfoliated Deciduous Teeth−

Tomoaki TAGUCHI1)6), Reira MINAMI2), Nozomi KAWASHIRI2), Sayaka MORI2), Koichiro YOSHIMARU3),

Yoshiaki TAKAHASHI3), Takayosi YAMAZA4) and Sachiyo SUITA5)6)

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Fukuoka College of Health Sciences

Department of Dental Hygiene, Fukuoka College of Health Sciences

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Department of Pediatric Surgery, Graduate School of Medical Sciences, Kyushu University

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Department of Molecular Cell Biology and Oral Anatomy, Graduate School of

Dental Sciences, Kyushu University

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Fukuoka Gakuen

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Professor Emeritus, Kyushu University

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Abstract

Regenerative medicine using stem cell can provide new treatment modalities for intractable

diseases. Stem cells from human exfoliated deciduous teeth (SHED) share some characteristics of

mesenchymal stem cells (MSCs), and exhibit self-renewal, multipotency into a variety of mature cells.

Recent studies have conducted to evaluate the immunomodulatory effects of SHED, which are

considered a feasible cell source for tissue engineering and regenerative medicine. In comparison with

other stem cells, SHED have the distinct advantages of minimal risk of oncogenesis and less ethical

controversy, and a readily accessible source, easy and minimally invasive collection. SHED have also

high stem cell potential such as cell proliferation, multipotency, and immunosuppressive ability even

after cryopreservation. Even in adult, we may use wisdom teeth or buried teeth for harvesting dental

pulp stem cell. Currently, some companies have been creating a SHED bank for allogenic cell therapy

in future. They are also collaborating us for clinical study using autologous SHED.

We have been trying to develop new treatments for pediatric intractable diseases, such as, intestinal

motility disorders (allied disorders of Hirschsprungʼs disease, liver failure and enzyme-deficient

diseases, and severe congenital diaphragmatic hernia, using cell therapy and tissue engineering

technology including SHED.

Key words：Regenerative medicine, Dental pulp stem cell, Hirschsprungʼs disease, Liver transplantation, Congenital diaphragmatic hernia

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